全文获取类型
收费全文 | 6653篇 |
免费 | 314篇 |
国内免费 | 26篇 |
专业分类
耳鼻咽喉 | 44篇 |
儿科学 | 149篇 |
妇产科学 | 84篇 |
基础医学 | 981篇 |
口腔科学 | 95篇 |
临床医学 | 418篇 |
内科学 | 1793篇 |
皮肤病学 | 72篇 |
神经病学 | 450篇 |
特种医学 | 243篇 |
外科学 | 872篇 |
综合类 | 36篇 |
一般理论 | 1篇 |
预防医学 | 243篇 |
眼科学 | 151篇 |
药学 | 610篇 |
中国医学 | 38篇 |
肿瘤学 | 713篇 |
出版年
2023年 | 39篇 |
2022年 | 66篇 |
2021年 | 108篇 |
2020年 | 68篇 |
2019年 | 93篇 |
2018年 | 130篇 |
2017年 | 78篇 |
2016年 | 120篇 |
2015年 | 125篇 |
2014年 | 183篇 |
2013年 | 171篇 |
2012年 | 302篇 |
2011年 | 364篇 |
2010年 | 213篇 |
2009年 | 177篇 |
2008年 | 317篇 |
2007年 | 367篇 |
2006年 | 372篇 |
2005年 | 398篇 |
2004年 | 381篇 |
2003年 | 418篇 |
2002年 | 385篇 |
2001年 | 150篇 |
2000年 | 163篇 |
1999年 | 165篇 |
1998年 | 100篇 |
1997年 | 74篇 |
1996年 | 82篇 |
1995年 | 55篇 |
1994年 | 63篇 |
1993年 | 75篇 |
1992年 | 111篇 |
1991年 | 90篇 |
1990年 | 94篇 |
1989年 | 94篇 |
1988年 | 65篇 |
1987年 | 88篇 |
1986年 | 87篇 |
1985年 | 67篇 |
1984年 | 66篇 |
1983年 | 57篇 |
1982年 | 31篇 |
1981年 | 30篇 |
1980年 | 26篇 |
1979年 | 43篇 |
1978年 | 28篇 |
1975年 | 25篇 |
1969年 | 23篇 |
1968年 | 18篇 |
1966年 | 21篇 |
排序方式: 共有6993条查询结果,搜索用时 124 毫秒
91.
Kanako Omata Noriki Okada Go Miyahara Yuta Hirata Yukihiro Sanada Yasuharu Onishi Shinya Fukuda Hideki Kumagai Alan Kawarai Lefor Yasunaru Sakuma Naohiro Sata 《Transplantation proceedings》2021,53(4):1317-1321
BackgroundMyotubular myopathy is a rare disease sometimes accompanied by peliosis hepatis, a leading cause of fatal liver hemorrhage.Case ReportWe present a case of a 2-year-old boy with myotubular myopathy who developed liver hemorrhage because of peliosis hepatis and was successfully treated with living-donor liver transplant. The patient initially presented with fever, anemia, and liver dysfunction. A computed tomographic scan revealed hemorrhages in the liver, and the patient underwent hepatic artery embolization twice. After the second embolization, multiple peliosis hepatis cavities appeared in the left lobe of the liver that had increased in size. Therefore, the patient underwent ABO-incompatible living-donor liver transplant using a lateral segment graft from his father. The patient developed severe septic shock with an unknown focus on postoperative day 18, which resolved with antibiotic therapy. On postoperative day 62, he was discharged. Fourteen months after undergoing living-donor liver transplant, the patient showed no recurrence of peliosis hepatis.ConclusionsAlthough the long-term prognosis of peliosis hepatis due to myotubular myopathy after living-donor liver transplant remains unclear, liver transplant may be a curative treatment for patients with myotubular myopathy who have uncontrollable peliosis hepatis. 相似文献
92.
Kai Nishime Chika Miyagi-Shiohira Kazuho Kuwae Yoshihito Tamaki Tasuku Yonaha Mayuko Sakai-Yonaha Issei Saitoh Masami Watanabe Hirofumi Noguchi 《American journal of transplantation》2021,21(8):2698-2708
Ischemia-reperfusion injury (IRI) results in increased rates of delayed graft function and early graft loss. It has recently been reported that hydrogen sulfide (H2S) protects organ grafts against prolonged IRI. Here, we investigated whether the preservation of pancreas in University of Wisconsin (UW) solution supplemented with AP39, which is a mitochondrial-targeted H2S donor, protected pancreatic islets against IRI and improved islet function. Porcine pancreata were preserved in the UW solution with AP39 (UW + AP39) or the vehicle (UW) for 18 h, followed by islet isolation. The islet yields before and after purification were significantly higher in the UW + AP39 group than in the UW group. The islets isolated from the pancreas preserved in UW + AP39 exhibited significantly decreased levels of reactive oxygen species (ROS) production and a significantly increased mitochondrial membrane potential as compared to the islets isolated from the pancreas preserved in the vehicle. We found that the pancreas preserved in UW + AP39 improved the outcome of islet transplantation in streptozotocin-induced diabetic mice. These results suggest that the preservation of pancreas in UW + AP39 protects the islet grafts against IRI and could thus serve as a novel clinical strategy for improving islet transplantation outcomes. 相似文献
93.
Kazuhide Kumagai Akira Yasui Yoshiaki Nishida Koki Masuo Akihiro Yoshitoshi 《Surgery today》1993,23(10):875-879
In order to achieve a complete prognosis for early gastric carcinoma, a greater effort must be made to improve its present treatment, considering the small percentage of patients who still die from recurrence despite the prompt initiation of surgery. Over the past 9 years, 26 patients with early gastric carcinoma have undergone surgical resection after receiving preoperative chemotherapy in the form of oral 5-FU or 5-DFUR in our institute. The effectiveness of preoperative chemotherapy was evaluated by histopathological examination of the resected stomachs. Of a total of 24 patients with depressed type gastric cancer, 19 were histologically found to have a cancerless area within the cancerous lesion, 8 of whom were classified as being over Grade 1b. Gross changes were observed in 13 of these 24 patients. The frequency of multiple early gastric cancer occurring in patients who had not received chemotherapy was 11.6%, whereas in those who had received chemotherapy it was 3.8%. The findings of this study thus indicate that preoperative chemotherapy is useful for reducing minute cancer foci and microscopic metastatic lesions. 相似文献
94.
Takagaki Masao Oda Yoshifumi Miyatake Shin-Ichi Kikuchi Haruhiko Kobayashi Toru Sakurai Yoshinori Osawa Masami Mori Kenjiro Ono Koji 《Journal of neuro-oncology》1997,35(2):177-185
To plan the optimal BNCT using BSH for glioblastoma patients, the10B concentration in tumor and blood was investigated in 11newly diagnosed glioblastoma patients. All patients received 20 mg BSH/kgbody weight 2.5–16 hrs prior to tumor removal. The quantitativedistribution of 10B was determined by prompt gamma rayspectrometry and/or -track autoradiography. 10Bdistribution in tumors was heterogeneous, ± 25% of scatteringat the microscopic level, and the distribution was also heterogeneous at thetissue level. 10B concentration in blood decreased inbi-exponential decay as a function of the time after the end of theadministration. The T/B ratio showed non-exponential increase with largevariation. The maximum T/B ratio would be around 1. The tumor/normal brain(T/N) ratio of 10B concentration was 11.0 ± 3.2. The10B content in normal brain is originated in vascular10B in parenchyma, since the 10B content innormal brain to blood (N/B ratio) being compatible with the blood content inparenchyma. These values allow for BNCT, using thermal neutrons, on braintumors located less than approximately 3.3 cm in depth from the brainsurface of neutron incidence, providing that the dose on the normalendothelium is controlled to less than the tolerance limit. In ourpreliminary study of BNCT, a 31% 3-year survival was achieved overall for 16 glioblastoma patients and a 50% 2-year survival wasachieved on 8 glioblastoma patients in our recent dose escalation studybased on these data. 相似文献
95.
Iwabuchi Satoru Handa Masashi Usuda Katsuo Sato Masami Kondo Takashi Tanita Tatsuo Fujimura Shigefumi 《Surgery today》1994,24(11):1014-1018
A 52-year-old Japanese man with a slow-growing chondroma originating from the sternal bone was referred to our hospital. A subtotal resection of the sternum was performed, hereafter termed the sandwich method, and an originally designed prosthesis made from ultra-high-molecular-weight polyethylene and Marlex mesh was used for reconstruction. The postoperative course was uneventful without any symptoms due to paradoxical movement of the chest or regional abscess, and no disturbance in the movement of the upper limbs, such as a surgical sequelae, was observed. 相似文献
96.
Ei Kawahara Kazushi Imai Shigehiro Kumagai Etsuhide Yamamoto Isao Nakanishi 《Journal of cancer research and clinical oncology》1995,121(3):133-140
We studied invasion-related adhesion events in vitro using three squamous carcinoma cell lines (HSC-3, poorly differentiated type; OSC-19, well-differentiated type; and KB cells, undifferentiated type). An in vitro invasion assay through matrigel in the transwell chamber revealed that HSC-3 cells were most invasive, OSC-19 cells moderately invasive and KB cells least invasive. Inhibition assay of invasion using synthetic peptides RGD, RGDV, RGDS, RGDT, IKVAV and YIGSR, showed that invasion of the three cell lines was significantly inhibited by RGDV. There were other peptides that inhibited invasion significantly including IKVAV for HSC-3, and RGDS and YIGSR for OSC-19. HSC-3 cells and OSC-19 cells adhered to fibronectin, laminin, vitronectin, and type IV collagen, and KB cells did not adhere to laminin but did to fibronectin, vitronectin and collagen type IV. Pretreatment of cells with RGDV peptide in the attachment assay reduced the ability of these cells to bind to vitronectin and fibronectin more efficiently than pretreatment with RGDS. Anti-v antibodies inhibited adhesion of HSC-3, OSC-19 and KB cells to vitronectin, but anti-1 antibodies did not inhibit adhesion. Immunofluorescent microscopic examinations showed that all cell lines were positive for anti-5 and anti-v antibodies, and only HSC-3 cells were positive for anti-3 antibody. 51 was not clearly demonstrated in any of the cell lines. RGDV was the most effective inhibitor of squamous cell carcinoma invasion among the synthetic oligopeptides used in this experiment, and it is suggested that it affects v3-and/or v5-mediated carcinoma cell invasion.Abbreviations
BSA
bovine serum albumin
-
MEM
Eagle's minimal essential medium
-
MTT
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
PBS
phosphatebuffered saline
-
FITC
fluorescein isothiocyanate
This work supported in part by a grant from the Osaka Cancer Research Foundation 相似文献
97.
Mishima K Sawada S Tanigawa N Okuda Y Kobayashi M Koyama T 《Cardiovascular and interventional radiology》1999,22(2):155-158
Four patients were treated by placement of an expandable metallic stent (two Gianturco Z-stents, two Ultraflex stents) for malignant colorectal strictures. All four patients were able to defecate after stent placement. Stent migration was recognized in one patient. Two patients suffered from tenesmus after stent placement. 相似文献
98.
Apoptotic cells associated with Wallerian degeneration after experimental spinal cord injury: a possible mechanism of oligodendroglial death. 总被引:14,自引:0,他引:14
Y Abe T Yamamoto Y Sugiyama T Watanabe N Saito H Kayama T Kumagai 《Journal of neurotrauma》1999,16(10):945-952
We have investigated the temporal and spatial profiles of apoptotic cells in an experimental transection spinal cord injury by the terminal deoxynucleotidyl transferase-mediated biotin-16-2'-deoxyuridine-5'-triphosphate nick-end labeling (TUNEL) method. Twenty-four hours postinjury, a numerous TUNEL-positive cells appeared both rostrally and caudally to the transection site. Those positive cells, however, gradually diminished in number by several days postinjury. In contrast, other TUNEL-positive cells were found scattered within the white matter remote from the lesion by the third day postinjury. These cells were typically embedded in or among vacuolated fibers, where they were identified in close proximity to the vacuolated space enclosed by myelin basic protein (MBP)-positive structures confirmed by TUNEL-MBP double staining. Because of their linear arrangement, these TUNEL-positive cells were considered interfascicular oligodendrocytes, a fact that was confirmed by the finding that some TUNEL-positive cells were also stained with CCI, a cell marker for oligodendrocyte. Electron microscopic studies revealed that the cells expressing apoptotic morphology were invariably encased in a space formed by myelin splitting. Although the biological significance of apoptotic interfascicular oligodendrocytes in the process of wallerian degeneration is yet to be determined, the finding of such profiles localized within degenerating myelin structures suggests that; oligodendrocytes may be "trapped" within rapidly swollen and disintegrating myelin lamellae, which isolates and perhaps predisposes them to death. 相似文献
99.
H Miyazawa S Saitoh T Kumagai T Yamanaka S Yasuda Y Tsunetsugu-Yokota S Inouye M Sakaguchi 《Vaccine》1999,17(17):2176-2180
We examined anti-gelatin IgG in sera of children who suffered from systemic adverse reactions upon immunization with gelatin-containing live virus vaccines. In the group of 30 children who had immediate-type reactions and anti-gelatin IgE, 30 (100%) had anti-gelatin IgG and 29 (96%) had anti-gelatin IgG4. In another group of 75 children who had nonimmediate-type reactions and no anti-gelatin IgE, 22 (29%) had anti-gelatin IgG and six (8%) had IgG4. The IgG positivity well correlated with the lymphocyte proliferation assay positivity. In contrast, as a negative control, all 24 children who had no allergic reaction to live virus vaccines had no anti-gelatin IgG and IgG4. The results suggest that immune-response to gelatin may play a role in the pathogenesis of systemic nonimmediate-type reactions to the live virus vaccines. 相似文献
100.