Spontaneous resolution of severe aplastic anemia following thymic hemorrhage

Over the last 7 years we have seen more than 200 severe aplastic anemia patients at this centre. Three of them developed an unusual complication in the form of thymic hemorrhage. Following this complication, all 3 patients recovered partially from their aplastic anemia, without any need for further immunosuppression. These cases show possible ways to manipulate the thymus gland as a management strategy for this disease.     

Congenital methemoglobinemia caused by Hb-MRatnagiri (beta-63CAT-->TAT, His-->Tyr) in an Indian family

Hb-M is a very rare hemoglobinopathy in the Indian subcontinent. We report a family with Hb-M with lifelong cyanosis from the Ratnagiri district in western India. The propositus was a 11-year-old female child with a history of increasing cyanosis exacerbated by fever and weakness. Similar complaints were also noted in her mother and five maternal family members. There was no history of cardiac illness or exposure to drugs and chemicals. The methemoglobin level was 39.3% in the propositus and 21.1% in her mother with normal NADH-methemoglobin reductase activity. Abnormal absorption peaks by spectroscopic analysis, presence of hemoglobin instability, and a slow-moving band on starch gel electrophoresis supported the presence of Hb-M. Automated DNA sequence analysis of the beta globin gene showed a C-->T substitution at codon 63. This leads to a substitution of histidine (CAT) by tyrosine (TAT) at the beta 63 (E7) position, similar to Hb-M Saskatoon. We have named this variant as Hb-M(Ratnagiri).     

Enhanced electron transfer mediated detection of hydrogen peroxide using a silver nanoparticle–reduced graphene oxide–polyaniline fabricated electrochemical sensor

The current study aims at the development of an electrochemical sensor based on a silver nanoparticle–reduced graphene oxide–polyaniline (AgNPs–rGO–PANI) nanocomposite for the sensitive and selective detection of hydrogen peroxide (H₂O₂). The nanocomposite was fabricated by simple in situ synthesis of PANI at the surface of rGO sheet which was followed by stirring with AEC biosynthesized AgNPs to form a nanocomposite. The AgNPs, GO, rGO, PANI, rGO–PANI, and AgNPs–rGO–PANI nanocomposite and their interaction were studied by UV-vis, FTIR, XRD, SEM, EDX and XPS analysis. AgNPs–rGO–PANI nanocomposite was loaded (0.5 mg cm⁻²) on a glassy carbon electrode (GCE) where the active surface area was maintained at 0.2 cm² for investigation of the electrochemical properties. It was found that AgNPs–rGO–PANI–GCE had high sensitivity towards the reduction of H₂O₂ than AgNPs–rGO which occurred at −0.4 V vs. SCE due to the presence of PANI (AgNPs have direct electronic interaction with N atom of the PANI backbone) which enhanced the rate of transfer of electron during the electrochemical reduction of H₂O₂. The calibration plots of H₂O₂ electrochemical detection was established in the range of 0.01 μM to 1000 μM (R² = 0.99) with a detection limit of 50 nM, the response time of about 5 s at a signal-to-noise ratio (S/N = 3). The sensitivity was calculated as 14.7 μA mM⁻¹ cm⁻² which indicated a significant potential as a non-enzymatic H₂O₂ sensor.

The current study aims at the development of an electrochemical sensor based on a silver nanoparticle–reduced graphene oxide–polyaniline (AgNPs–rGO–PANI) nanocomposite for the sensitive and selective detection of hydrogen peroxide (H₂O₂).     

Changing Gender Norms and Reducing Intimate Partner Violence: Results From a Quasi-Experimental Intervention Study With Young Men in Ethiopia

Objectives. We assessed the effects of a community-based project in Ethiopia that worked with young men to promote gender-equitable norms and reductions in intimate partner violence (IPV).Methods. A quasi-experimental design was used to assign young Ethiopian men 15 to 24 years of age (809 participants were surveyed at baseline in 2008) to an intervention involving community engagement (CE) activities in combination with interactive group education (GE) sessions promoting gender-equitable norms and violence prevention, an intervention involving CE activities alone, or a comparison group.Results. Participants in the GE + CE intervention were twice as likely (P < .01) as those in the comparison group to show increased support for gender-equitable norms between the baseline and end-line points. Also, the percentage of GE + CE participants who reported IPV toward their partner in the preceding 6 months decreased from 53% to 38% between baseline and end line, and the percentage in the CE-only group decreased from 60% to 37%; changes were negligible in the comparison group.Conclusions. Promoting gender equity is an important strategy to reduce IPV.In addition to being a human rights violation, gender-based violence (GBV) is a widespread public health issue with numerous negative health consequences. GBV, including intimate partner violence (IPV), causes injury and death and decreases survivors’ use of health services.1,2 For example, violence and fear of violence can be barriers to effective HIV prevention, care, and treatment programs. IPV has also been associated with a lack of contraception use and HIV acquisition.3,4 Consequently, addressing and reducing GBV is an explicit component of the President’s Emergency Plan for AIDS Relief.5The number of women and girls affected by IPV is enormous. A seminal study conducted in 10 different countries demonstrated that between 15% and 71% of women of reproductive age had experienced physical or sexual IPV (and estimated that 30 % of women globally had experienced IPV).6,7 The highest rates of IPV were those among Ethiopian women: 53.7% had experienced IPV within the 12 months preceding the interview and 70.9 % over their lifetime. In addition, HIV prevalence in Ethiopia is higher among women (1.9 %) than men (1.0 %).8Certain gender norms—or social expectations about men’s and women’s appropriate roles, rights, and responsibilities—have been shown to be associated with the risk of IPV as well as the risk of HIV and other sexually transmitted infections (STIs).9–12 For example, 45% of men and 68% of women taking part in the 2011 Ethiopia Demographic and Health Survey reported that wife beating is justified for at least 1 reason.8Over the past decade, a series of programs attempting to address inequitable gender norms have been implemented across the globe; many have emphasized the importance of engaging boys and men in this process.13,14 A growing body of work has demonstrated that these gender-focused interventions can lead to reductions in violence and to other positive health outcomes (e.g., increased contraception or condom use).9,15–19 However, rigorous evaluations of only a small number of these programs have been documented in the scientific literature, and recent literature reviews have revealed that existing evaluations have various limitations, including a lack of comparison groups and standardized or validated measures, no exploration of effects across types of IPV, inadequate follow-up rates, and limited use of theoretical frameworks.16,17To help address these gaps, we describe the results of a theoretically grounded, quasi-experimental intervention study of a community-based project in Ethiopia, the Male Norms Initiative, that worked with young men to promote gender-equitable norms and reductions in IPV. Our goal was to assess the effects of the intervention using standardized measures of violence and gender norms.The 2 main intervention components were interactive group education and community mobilization and engagement activities aimed at raising awareness and promoting community dialogue. The interventions focused on promoting critical reflection regarding common gender norms that might increase the risk of violence or HIV and other STIs (e.g., support for multiple sexual partners and acceptance of partner violence). Through this reflection, the participants were able to identify the potential negative outcomes of enacting these norms and the potential positive aspects of more gender-equitable behavior.In addition, the activities engaged the wider community in supporting a shift in specific harmful norms. Engaging Boys and Men in Gender Transformation, a manual based on EngenderHealth and Promundo’s gender-transformative programming, was used to facilitate this process.20 Hiwot Ethiopia, a nongovernmental organization, led the implementation of the project with technical support from EngenderHealth. PATH led the evaluation in collaboration with Miz-Hasab, an Ethiopia-based research institute.Both intervention groups participated in community engagement (CE) activities, which took place over a 6-month period from June to November 2008. Beginning with a march on International Father’s Day, these activities involved entire communities and included distribution of monthly newsletters and leaflets (approximately 15 000 in total), music and drama skits reaching 8700 people, monthly community workshop meetings, and distribution of more than 1000 condoms.A second component of the intervention—group education (GE) activities—took place over 4 months at youth centers during regularly scheduled youth group hours, usually on weekends. The activities included role plays, group discussions, and personal reflection. Sessions enrolling about 20 participants were facilitated by 2 or 3 peer educators each, with oversight from a master trainer. In total, 8 sessions 2 or 3 hours in duration were conducted, drawing on 19 activities from the manual.This intervention and evaluation design was informed by the theory of gender and power, a social structural theory that addresses environmental and social issues relating to gender dynamics, particularly sexual division of labor, sexual division of power, and the structure of cathexis (which is similar to the concept of gender norms).21 According to this theory, various negative health and other outcomes stem from the socialization of women to be sexually passive, women’s economic reliance on men, and abusive partnerships. The theory affirms—as does the empirical evidence provided in the introduction—that addressing gender norms is a core factor in reducing both IPV and related health risks such as HIV and other STIs.22     

A randomized,double-blind study of SHP465 mixed amphetamine salts extended-release in adults with ADHD using a simulated adult workplace design

Objectives: The objective of this paper was to evaluate the efficacy, duration of effect, and tolerability of SHP465 mixed amphetamine salts (MAS) extended-release versus placebo and immediate-release MAS (MAS IR) in adults with attention-deficit/hyperactivity disorder (ADHD).

Methods: Adults with ADHD Rating Scale, Version IV (ADHD-RS-IV) scores ≥24 were randomized to SHP465 MAS (50 or 75 mg), placebo, or 25 mg MAS IR in a double-blind, three-period, crossover study using a simulated adult workplace environment. On the final day of each 7-day treatment period, efficacy was assessed for 16 h postdose. Primary efficacy analyses for Permanent Product Measure of Performance (PERMP) total score averaged across all postdose assessments and each postdose time point were conducted in the intent-to-treat population using a mixed linear model. Secondary end-points included PERMP problems attempted and answered correctly and ADHD-RS-IV scores based on clinician ratings of counselor observations using the Time Segment Rating System and participant self-report. Tolerability assessments included treatment-emergent adverse events (TEAEs) and vital signs.

Results: Least squares mean (95% CI) treatment differences (combined 50/75 mg SHP465 MAS–placebo) significantly favored SHP465 MAS over placebo for PERMP total score averaged across all postdose assessments (18.38 [11.28, 25.47]; P < .0001) and at each postdose assessment (all P < .02). Nominal superiority of MAS IR over placebo for PERMP total score averaged across all postdose assessments was observed (nominal P = .0001); treatment differences between SHP465 MAS and MAS IR were not significant (nominal P = .2443). The two most frequently reported TEAEs associated with SHP465 MAS were insomnia (36.5%) and anorexia (21.2%). Mean increases in pulse and blood pressure with SHP465 MAS exceeded those of placebo.

Conclusions: SHP465 MAS (combined 50/75 mg) significantly improved PERMP total score versus placebo, with superiority observed from 2 to 16 h postdose. The tolerability profile of SHP465 MAS was similar to previous reports of SHP465 MAS in adults with ADHD.

Clinical trial registration: https://clinicaltrials.gov/ct2/show/NCT00928148 identifier is NCT00928148.