全文获取类型
收费全文 | 4173篇 |
免费 | 167篇 |
国内免费 | 27篇 |
专业分类
耳鼻咽喉 | 178篇 |
儿科学 | 89篇 |
妇产科学 | 134篇 |
基础医学 | 609篇 |
口腔科学 | 34篇 |
临床医学 | 375篇 |
内科学 | 1109篇 |
皮肤病学 | 24篇 |
神经病学 | 283篇 |
特种医学 | 132篇 |
外科学 | 494篇 |
综合类 | 40篇 |
一般理论 | 3篇 |
预防医学 | 192篇 |
眼科学 | 90篇 |
药学 | 312篇 |
中国医学 | 2篇 |
肿瘤学 | 267篇 |
出版年
2023年 | 29篇 |
2022年 | 157篇 |
2021年 | 250篇 |
2020年 | 67篇 |
2019年 | 107篇 |
2018年 | 137篇 |
2017年 | 84篇 |
2016年 | 105篇 |
2015年 | 114篇 |
2014年 | 172篇 |
2013年 | 218篇 |
2012年 | 329篇 |
2011年 | 369篇 |
2010年 | 177篇 |
2009年 | 114篇 |
2008年 | 241篇 |
2007年 | 255篇 |
2006年 | 277篇 |
2005年 | 240篇 |
2004年 | 240篇 |
2003年 | 194篇 |
2002年 | 148篇 |
2001年 | 27篇 |
2000年 | 24篇 |
1999年 | 21篇 |
1998年 | 17篇 |
1997年 | 13篇 |
1996年 | 11篇 |
1995年 | 15篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 10篇 |
1991年 | 12篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 17篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 10篇 |
1982年 | 5篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1975年 | 10篇 |
1973年 | 6篇 |
1972年 | 9篇 |
1971年 | 5篇 |
1970年 | 5篇 |
1965年 | 4篇 |
排序方式: 共有4367条查询结果,搜索用时 15 毫秒
41.
Maciej Cabanski Brett Fields Stephanie Boue Natalia Boukharov Hector DeLeon Natalie Dror Marcel Geertz Emmanuel Guedj Anita Iskandar Ulrike Kogel Celine Merg Michael J. Peck Carine Poussin Walter K. Schlage Marja Talikka Nikolai V. Ivanov Julia Hoeng Manuel C. Peitsch 《Inflammation research》2015,64(7):471-486
42.
The PCBP1 gene encoding poly(rc) binding protein i is recurrently mutated in Burkitt lymphoma 下载免费PDF全文
Rabea Wagener Sietse M. Aukema Matthias Schlesner Andrea Haake Birgit Burkhardt Alexander Claviez Hans G. Drexler Michael Hummel Markus Kreuz Markus Loeffler Maciej Rosolowski Cristina Lpez Peter Mller Julia Richter Marius Rohde Matthew J. Betts Robert B. Russell Stephan H. Bernhart Steve Hoffmann Philip Rosenstiel Markus Schilhabel Monika Szczepanowski Lorenz Trümper Wolfram Klapper Reiner Siebert 《Genes, chromosomes & cancer》2015,54(9):555-564
The genetic hallmark of Burkitt lymphoma is the translocation t(8;14)(q24;q32), or one of its light chain variants, resulting in IG‐MYC juxtaposition. However, these translocations alone are insufficient to drive lymphomagenesis, which requires additional genetic changes for malignant transformation. Recent studies of Burkitt lymphoma using next generation sequencing approaches have identified various recurrently mutated genes including ID3, TCF3, CCND3, and TP53. Here, by using similar approaches, we show that PCBP1 is a recurrently mutated gene in Burkitt lymphoma. By whole‐genome sequencing, we identified somatic mutations in PCBP1 in 3/17 (18%) Burkitt lymphomas. We confirmed the recurrence of PCBP1 mutations by Sanger sequencing in an independent validation cohort, finding mutations in 3/28 (11%) Burkitt lymphomas and in 6/16 (38%) Burkitt lymphoma cell lines. PCBP1 is an intron‐less gene encoding the 356 amino acid poly(rC) binding protein 1, which contains three K‐Homology (KH) domains and two nuclear localization signals. The mutations predominantly (10/12, 83%) affect the KH III domain, either by complete domain loss or amino acid changes. Thus, these changes are predicted to alter the various functions of PCBP1, including nuclear trafficking and pre‐mRNA splicing. Remarkably, all six primary Burkitt lymphomas with a PCBP1 mutation expressed MUM1/IRF4, which is otherwise detected in around 20–40% of Burkitt lymphomas. We conclude that PCBP1 mutations are recurrent in Burkitt lymphomas and might contribute, in cooperation with other mutations, to its pathogenesis. © 2015 Wiley Periodicals, Inc. 相似文献
43.
Tomasz Cytlak Monika Skibiska Patrycja Kaczmarek Marcin Ka
mierczak Magdalena Rapp Maciej Kubicki Henryk Koroniak 《RSC advances》2018,8(22):11957
Direct conversion of the α-hydroxyl group by para-toluenesulfonamide to yield α-(N-tosyl)aminophosphonates is reported. α-Aminophosphonates 23a,b–37a,b were obtained from the corresponding α-hydroxyphosphonates 6a,b–21a,b in the presence of K2CO3, via the retro-Abramov reaction of the appropriate aldehydes, 1–5. The subsequent formation of imines with simultaneous addition of diethyl phosphite provided access to the α-sulfonamide phosphonates 23a,b–37a,b with better diastereoselectivity than in the case of the Pudovik reaction. The mechanism for this transformation is proposed herein. When Cbz N-protected aziridine 9a,b and phenylalanine analogue 12a,b were exploited, intramolecular substitution was observed, leading to the corresponding epoxide 38 as the sole product, or oxazolidin-2-one 39 as a minor product. Analogous substitution was not observed in the case of proline 18a,b and serine 21a,b derivatives.The reaction mechanism and diastereoselectivity of the direct transformation of α-hydroxyphosphonates 6a,b–21a,b by para-toluenesulfonamide, yielding α-(N-tosyl)aminophosphonates 23a,b–37a,b under K2CO3 conditions are presented. 相似文献
44.
Maciej Dzwonek Dominika Zaubiniak Piotr Pitek Grzegorz Cichowicz Sylwia Mczynska-Wielgosz Tomasz Stpkowski Marcin Kruszewski Agnieszka Wickowska Renata Bilewicz 《RSC advances》2018,8(27):14947
Modification of ultrasmall gold nanoparticles (AuNPs) with the lipoic acid derivative of folic acid was found to enhance their accumulation in the cancer cell, as compared to AuNPs without addressing units. The application of lipoic acid enabled the control of the gold nanoparticle functionalities leading to enhanced solubility and allowing for attachment of both the folic acid and the cytotoxic drug, doxorubicin. More robust attachment of doxorubicin to the nanoparticle through the amide bond resulted in toxicity comparable with that of the drug alone, opening a new perspective for designing more potent, but less toxic nanopharmaceuticals. The increased uptake was accompanied by pronounced nuclear accumulation and observable cytotoxicity. Doxorubicin binding via covalent amide bonds enhanced stability of the whole drug vehicle and provided much better control over doxorubicin release in the cell environment, as compared to physical adsorption or pH sensitive bonding commonly used for anthracycline carriers. Confocal microscopy revealed that the bond was stable in the cytoplasm for 22 h. The ability to slow down the rate of drug release may be crucial for the application in sustained anticancer drug delivery. Biological analyses performed using MTT assay and confocal microscopy confirmed that the ultrasmall AuNPs with the lipoic acid derivative of folic acid exhibit relatively low cytotoxicity, however when loaded with a chemotherapeutic, they cause a significant reduction in the cell viability.Modification of ultrasmall gold nanoparticles (AuNPs) with the lipoic acid derivative of folic acid was found to enhance their accumulation in the cancer cell, as compared to AuNPs without addressing units. 相似文献
45.
Orally administered novel cyclic pentapeptide P‐317 alleviates symptoms of diarrhoea‐predominant irritable bowel syndrome 下载免费PDF全文
46.
Witold Kozak Mateusz Daśko Maciej Masłyk Konrad Kubiński Janusz Rachon Sebastian Demkowicz 《Drug development research》2015,76(8):450-462
Preclinical Research |
47.
48.
49.