Oritin and properties of the blister formation factor in blister fluids from recessive dystrophic epidermolysis bullosa

The origin and properties of the blister formation factor in recessive dystrophic epidermolysis bullosa (RDEB) blister fluids were investigated. Organ cultures of normal human skin incubated with RDEB dermis extract or with RDEB fibroblast culture medium (FCM) produced a clear subepidermal blister with histology similar to that of a RDEB blister in vivo. The injection of RDEB dermis extract into guinea-pig skin also induced dermal-epidermal separation with similar histology to the skin lesions of RDEB patients. The blister forming activity of RDEB FCM which induces the subepidermal blister was inactivated by heat (60 degrees C for 30 min), trypsin digestion and by treating with EDTA, EGTA, alpha 2-macroglobulin, diisopropyl fluorophosphate and N-ethylmaleimide, but was not affected by dialysis. These results suggest that the RDEB fibroblast produces a blister formation factor(s), and that blister formation may be caused by a combination of a metallo-protease, serine protease and SH protease.     

大鼠后肢热诱导的缓激肽和P物质的释放

用放射免疫法测定缓激肽(BK)和P物质(SP)含量,发现正常大鼠(B/N-Ki)左后肢用47℃,热水刺激20 min,后肢足跖皮下灌流液中BK和SP含量分别为43±34和11.1±9.7fmol·min~(-1),比热刺激前显著增加,但在血浆激肽原缺乏大鼠(B/N-Ka),BK和SP含量分别为1.3±1.0和5.5±3.5fmol·min~(-1),明显低于B/N-Ki大鼠。B/N-Ka大鼠的E-vans蓝漏出量也低于B/N-Ki大鼠。表明热刺激时产生的BK可介导SP的释放。     

A case control study of female systemic lupus erythematosus in Japan

In a multi-institutional case-control study, we investigatedrisk factors for systemic lupus erythematosus (SLE) in 151 Japanesewomen treated for SLE during the period September 1988 to August1990. We used two control groups: sisters of SLE patients andsisters of female patients with another disease. Informationwas obtained by a self-administered questionnaire. Our findingssuggest that having a history of operations and blood transfusionsand working in a cold environment may be risk factors for SLE.Viral infections, psychological stress, and pet ownership werenot risk factors for SLE in our study. In addition, smokingwas not protective for SLE. Skin sensitivity to sunlight andcold feet, which may be symptoms of SLE that occurred beforethe diagnosis, were also positively associated with SLE.     

Endoscopic ultrasonographic findings in rectal leiomyoma

Abstract A 35 year old man visiting a hospital for his annual check-up in August 1992 was found to have a large rectal tumour on digital examination. Colonoscopy revealed a bulging lesion with normal mucosa. Endoscopic biopsy showed only normal tissue. Endoscopic ultrasonography demonstrated a large hypo-echoic submucosal tumour in the fourth layer (muscularis propria) of the rectal wall. Based on this endoscopic ultrasonographic finding, we diagnosed the tumour as leiomyoma pre-operatively. The tumour was excised by a trans-sacral local excision. The histological diagnosis of the resected specimen was cellular leiomyoma.