1例系统性红斑狼疮患者视网膜中央动脉阻塞及脑出血的护理

对1例系统性红斑狼疮并发视网膜中央动脉阻塞及脑出血患者，给予糖皮质激素、免疫抑制剂、高压氧、改善微循环及对症支持治疗．同时予以密切观察病情、药物护理、高压氧治疗护理及健康教育等措施，结果患者恢复良好出院。提出该病虽然少见且后果严重，但及时采取综合性治疗护理措施，可以提高治疗效果。     

踝关节骨折腓骨钢板的后方固定

目的：探讨踝关节骨折中腓骨钢板固定的位置。方法：2001年1月～2003年1月共手术治疗踝关节骨折患者75例，仅对其中随访资料完整的48例患者进行分析。随访10～24个月，平均15个月。采用Baird和Jackson的踝关节评分系统评定踝关节术后疗效。结果：所有的患者均取得了良好的骨折愈合。术后疗效为优24例，良17例，可5例，差2例。优良率为85、4％。结论：腓骨钢板的后方放置可以减少缝合时的张力，钢板有更好的软组织覆盖，钢板无须过多塑形，固定比较牢固。     

应用HMM和加权距离判别法的真核基因识别程序研究

根据现代科学对基因的认识，应用了隐马尔科夫模型(HMM)的算法，以大量核酸序列为信息来源，通过计算机计算来寻找未知基因的大体位置；再通过基因的固有结构特征及密码子使用的偏向性，使用加权距离判别法来准确地定位基因，以图形及文本的形式输出，从而极大地方便了实验室的研究工作。考虑到基因的许多特征还不为人们所了解，而且不同物种之间基因结构又有一定的差异，所以还开发了程序自学习功能，不断地存储已知的基因，再据此改变一些已有固有数据，以便更好地适应和了解不同生物基因结构的特异性，更加准确地寻找未知基因的位置。     

碳纤维根管桩的研制

目的研究高强度碳纤维增强复合树脂根管桩的加工成型工艺.方法用湿法制备碳纤维预浸料,在预成型模具中预成型,然后在成型模具中经特殊的工艺进行成型,最后用车床加工成预定形状的桩钉;并测试其弯曲强度、剪切强度和碳纤维含量.结果随着碳纤维含量的增加,根管桩的弯曲强度和剪切强度均逐渐增加.碳纤维含量达到67.13%时,弯曲强度和剪切强度分别达到406MPa和653.86MPa.结论高强度的碳纤维根管桩可以满足临床需要.     

Expression of Anion Exchanger 1 Sequestrates p16 in the Cytoplasm in Gastric and Colonic Adenocarcinoma

p16^INK4A (p16) binds to cyclin-dependent kinase 4/6 and negatively regulates cell growth. Recent studies have led to an understanding of additional biologic functions for p16; however, the detailed mechanisms involved are still elusive. In this article, we show an unexpected expression of anion exchanger 1 (AE1) in the cytoplasm in poorly and moderately differentiated gastric and colonic adenocarcinoma cells and in its interaction with p16, thereby sequestrating the protein in the cytoplasm. Genetic alterations of p16 and AE1 were not detectable. Forced expression of AE1 in these cells sequestrated more p16 in the cytoplasm, whereas small interfering RNA-mediated silencing of AE1 in the cells induced the release of p16 from the cytoplasm to the nucleus, leading to cell death and growth inhibition of tumor cells. By analyzing tissue samples obtained from patients with gastric and colonic cancers, we found that 83.33% of gastric cancers and 56.52% of colonic cancers coexpressed AE1 and p16 in the cytoplasm. We conclude that AE1 plays a crucial role in the pathogenesis of gastric and colonic adenocarcinoma and that p16 dysfunction is a novel pathway of carcinogenesis.     

不伴Ⅱb型多发性内分泌肿瘤综合征的咽喉部多发性黏膜神经瘤一例

不伴Ⅱb型多发性内分泌肿瘤综合征(multiple endocrine neoplazia Ⅱb，MENⅡb)的多发性黏膜神经瘤(multiple mucosal neuroma，MMN)甚为罕见，现有文献仅见5例报道，病变分别位于结膜、支气管、舌、直肠乙状结肠和喉部。我们收治1例不伴MENⅡb的咽喉部MMN，报道如下。     

Characteristics of the Nonselective Cation Current （NSCCs） in Rabbit Left Ventricular Epicardial, Midmyocardial and Endocardial Myocytes

Objectives Recent studies have described regional differences in the electrophysiology and pharmacology of ventricular myocardium in canine, feline, rat, guinea pig, and human hearts. This has been shown to be due to a smaller IKs and a lager sodium-calcium exchange current (INa-Ca) and late INa in M region (deep subepicardial to midmyocardial). Studies from our laboratory have found a new repolarization current-nonselective cation current (NSCCs) existing in rabbit right ventricular myocytes. Methods We examined the characteristics of NSCCs in epicardial, M region, and endocardial cells isolated from the rabbit left ventricle with standard microelectrode and whole-cell patch-clamp techniques. The permeability to Na , K , Li , Cs but not to Cl- indicating that it was a nonselective cation current. Gd3 (0.1 mmol/l) and La3 (0.1 mmol/l) can block the current markedly. Results Further characterization of NSCCs was significantly smaller in M cells than in epicardial and endocardial cells. NSCCs current density was significantly smaller in M cells than in epicardial and endocardial cells. With repolarization to -80 mV, INs current density was (-0.44±0.05) PA/PF in endocardial cells, (-0.12±0.05) PA/PF in M cells and (-0.28±0.07) PA/PF in epicardial cells; and with repolarization to 30 mV, INs current density was (1.09±0.29) PA/PF in endocardial cells, (0.38±0.09) PA/PF in M cells and (0.91±0.32) PA/PF in epicardial cells. Conclusions Transmural dispersion of repolarization was due to the heterogeneity of NSCCs in rabbit left ventricle epicardial, endocardial myocytes and M cells. These findings may advance our understanding of the ionic basis for our understanding of factors contributing to the development of cardiac arrhythmias.     

应用上唇外侧组织瓣旋转修复下唇缺损

目的:探求上唇外侧组织瓣在修复较大面积下唇缺损中的作用。方法:根据患者下唇缺损情况,设计上唇外侧组织瓣,旋转后插入缺损组织,同时在缺损吻合处,将上层外侧旋转瓣内侧粘膜肌肉向外牵拉形成部分新的红唇,然后逐层相对缝合,修复缺损。结果:自2003年1月应用此方法修复患者6例,术后随访1～2年,唇部外形好,无流涎,无口闭合不全,效果满意。     

腹水超滤浓缩回输系统临床应用评价

目的探讨腹水超滤浓缩回输腹腔系统的临床应用价值.方法对29例顽固性腹水患者在左下腹、右中腹进行腹腔穿刺,并将穿刺针与导管连接固定,腹水从左下腹引入动脉导管,通过滤过器在负压泵作用下形成超滤,水及中、小分子等滤出,浓缩后的腹水经静脉导管自右下腹回输入腹腔.结果超滤后患者体重下降,腹围缩小,尿量增加,血中肌酐下降,总蛋白、白蛋白上升(p＜0.05),结论腹水超滤回输有效、安全、简便、副作用少.