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21.
Kida T Nishihira Y Hatta A Wasaka T Nakata H Sakamoto M Nakajima T 《European journal of applied physiology》2003,89(3-4):326-330
We investigated the relationship between somatosensory event-related potentials (ERP) and the variation of reaction time (RT).
For this purpose, we recorded the ERPs (N250 and P300) in fast- and slow-reaction trials during a somatosensory discrimination
task. Strong, standard, and weak target electrical stimuli were randomly delivered to the left median nerve at the wrist with
a random interstimulus interval (900–1,100 ms). All the subjects were instructed to respond by pressing a button with their
right thumb as fast as possible whenever a target stimulus was presented. We divided all the trials into fast- and slow-RT
trials and averaged the data. N250 latency tended to be delayed when the RT was slow, but not significantly. P300 latency
was delayed significantly when the RT was slow, but to a much lesser extent than the RT delay, so we concluded that the change
of RT was not fully determined by the processes reflected by the somatosensory N250 or P300. Furthermore, the larger and earlier
P300 in the fast-RT trials implied that when larger amounts of attentional resources were allocated to a given task, the speed
of stimulus evaluation somewhat increased and RT was shortened to a great extent. N250 amplitude did not significantly vary
in the two RT clusters. In conclusion, the somatosensory N250 reflects active target detection, which is relatively independent
of the modulation of the response speed, whereas the somatosensory P300 could change without manipulation of either the stimulus
or the response processing demand.
Electronic Publication 相似文献
22.
Goya S Matsuoka H Mori M Morishita H Kida H Kobashi Y Kato T Taguchi Y Osaki T Tachibana I Nishimoto N Yoshizaki K Kawase I Hayashi S 《The Journal of pathology》2003,200(1):82-87
A variety of pathological changes are seen in lymphoproliferative disorders of the lung but the histogenesis of these abnormalities is not yet fully understood. We previously showed that adenovirus vector-mediated transient expression of both the human interleukin-6 (IL-6) and IL-6 receptor (IL-6R) genes, but not the IL-6 gene alone, in the rat lung induced lymphocytic alveolitis. In the present study, we explored the lung pathology of human IL-6 and IL-6R double transgenic mice to elucidate the effects of prolonged IL-6 signalling on the lung. The transgenic animals developed mononuclear cell accumulation in peribronchovascular regions, but little infiltration into alveolar spaces. Immunohistochemical analysis revealed that the cellular accumulations contained not only mixtures of inflammatory cells but also lymphoid tissue-like structures. As the expression of CXCL13/BLC, the indispensable chemokine for lymphoid organogenesis, was recognized in the B cell follicles of the pulmonary lesions, we speculate that this chemokine plays an inductive role in the development of the lymphoid tissue-like structures. These structures were distinguished from bronchus-associated lymphoid tissues (BALTs) by their location and by the lack of lymphoepithelium, which is a characteristic of BALT. These findings imply that IL-6 signalling may play a role in the pathogenesis of lymphoproliferative disorders of the lung. 相似文献
23.
Eiko Arai Shuichi Nishimura Kenichi Tamura Mitsushiro Kida Tatsuro Ikeuchi 《Journal of human genetics》1994,39(4):433-437
Summary Distal deletion of chromosome 1q has been reported in nearly 30 patients, all being associated with a deletion ranging from the 1q42 or q43 band to 1qter region. Here, we describe a girl with 1q terminal deletion resulting from an unbalancedde novo translocation t(1;D or G)(q44; p11), as revealed by the presence of a satellited feature and an NOR-stained region at the tip of 1q. We suggest that most of the phenotypic abnormalities seen in patients with 1q distal deletion are attributable to the monosomy for band 1q44. 相似文献
24.
Acquired gain of an X chromosome as the sole abnormality in the blast crisis of chronic neutrophilic leukemia 总被引:2,自引:0,他引:2
Chronic neutrophilic leukemia (CNL) is a rare myeloproliferative disorder characterized by sustained neutrophilic leukocytosis and absence of the Philadelphia chromosome. Most patients with CNL have normal karyotypes, and no specific cytogenetic abnormality has been identified. We report here a patient with CNL that evolved to myeloid blast crisis. A 73-year-old man was admitted to the hospital because of marked leukocytosis (leukocyte count 112.5 x 10(9)/L with 91% segmented neutrophils) and massive hepatosplenomegaly that was diagnosed as CNL with a normal karyotype. After treatment with hydroxyurea for 7 months, the disease progressed to a blast crisis. Bone marrow showed myeloid hyperplasia with 21% myeloblasts, 15% promyelocytes, and marked dysplastic changes of neutrophils. Blastic cells were positive for CD10, CD13, CD14, CD33, CD34, and HLA-DR. Chromosome analysis of the bone marrow cells showed 46,XY,+X in all 20 metaphase spreads. We reviewed 15 cases of CNL terminating in the blast crisis and confirmed that all cases transformed into myeloid crises and had poor prognoses. Furthermore, to our knowledge, this is the first case showing the acquired gain of an extra X chromosome as a sole abnormality in CNL. The gain of an extra X chromosome may play an important role in the progression from chronic phase to the blast crisis of CNL. 相似文献
25.
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27.
Ishiguro N Takada A Yoshioka M Ma X Kikuta H Kida H Kobayashi K 《Archives of virology》2004,149(1):17-34
Primary human umbilical vein endothelial cells (HUVECs) were infected with Influenza virus A/Aichi/2/68 (H3N2) in order to determine the role of endothelial cells in mediating inflammation induced upon virus infection. Structural proteins of the virus and mRNA of the M2 protein were detected in the infected cells, indicating that virus infection had occurred in HUVECs. The Influenza A virus-infected HUVECs showed elevated levels of gene expression of interferon (IFN)-inducible protein (IP)-10 and monokine induced by IFN-gamma (Mig), while heat-, formalin- and diethyl ether-inactivated viruses did not enhance the IP-10 and Mig gene expression. The results thus indicate that infection of live Influenza A virus is responsible for elevation of IP-10 and Mig gene expression. The elevation of IP-10 and Mig gene expression in infected HUVECs was not accompanied by the elevation of IFN-gamma gene expression, indicating that the elevation of IP-10 and Mig gene expression was independent of the IFN-gamma pathway. 相似文献
28.
Okazaki K Takada A Ito T Imai M Takakuwa H Hatta M Ozaki H Tanizaki T Nagano T Ninomiya A Demenev VA Tyaptirganov MM Karatayeva TD Yamnikova SS Lvov DK Kida H 《Archives of virology》2000,145(5):885-893
Summary. Influenza A viruses of different subtypes were isolated from fecal samples of ducks in their nesting areas in Siberia in
summer from 1996 to 1998. Phylogenetic analysis of the NP genes of the isolates in Siberia and those in Hokkaido, Japan on
their flyway of migration from Siberia to the south in autumn revealed that they belong to the Eurasian lineage of avian influenza
viruses. It is noted that the genes of the isolates in Siberia are closely related to those of H5N1 influenza virus strains
isolated from chickens and humans in Hong Kong in 1997 as well as to those of isolates from domestic birds in southern China.
The results indicate that influenza viruses perpetuated in ducks nesting in Siberia should have contributed genes in the emergence
of the H5N1 virusin Hong Kong. Vaccine prepared from avirulent A/duck/Hokkaido/4/96 (H5N3) influenza virus was potent enough
to protect mice from challenge with lethal dose of the pathogenic H5N1 virus [19]. Intensive surveillance study of aquatic
birds especially in Siberia is, therefore, stressed to provide information on the future pandemic influenza virus strains
and for vaccine preparation.
Received August 24, 1999/Accepted January 7, 2000 相似文献
29.
Koichi Yamada Hiroko Inoue Satoshi Kida Shoichi Masushige Tatsuaki Nishiyama Kenji Mishima Ichiro Saito 《Pathobiology》2006,73(1):1-7
OBJECTIVE: Saliva secretion is mediated by cAMP and the calcium signaling pathway in salivary acinar cells. The PKA signaling pathway plays an important role in protein secretion through the activation of cAMP, in fluid secretion through the elevation of intracellular calcium and in the activation of cAMP response element-binding protein (CREB), which is involved in these signaling cascades. In this study, we investigated whether the activation of CREB plays a part in the salivary secretion in mice. METHODS: We examined CREB activation by assessing phosphorylation at the serine-133 position using Western blotting. RESULTS: Carbachol (a muscarinic acetylcholine agonist) and isoproterenol (a beta-adrenergic agonist) markedly activated CREB in parotid acinar cells. Carbachol and isoproterenol-induced CREB phosphorylation was blocked by atropine (a muscarinic acetylcholine antagonist) and propranolol (a beta-adrenergic antagonist), respectively. The PKA inhibitor H89 inhibited CREB activation, but the PLC inhibitor U73122 did not. Moreover, carbachol- and isoproterenol-stimulated amylase secretion from parotid acinar cells was inhibited by H89 and adenoviral dominant-negative CREB. CONCLUSION: These results indicate that the muscarinic and beta-adrenergic activation of CREB was mediated through the PKA pathway and that CREB is involved in protein secretion from parotid acinar cells. 相似文献
30.
Electron-dense deposition patterns and the outcomes of idiopathic membranous nephropathy in Japanese 总被引:1,自引:0,他引:1
Hitoshi Yokoyama Keiichi Yoshimoto Takashi Wada Kengo Furuichi Satoshi Goshima Hiroshi Kida Ken-ichi Kobayashi 《Medical Electron Microscopy》2002,35(2):81-86
A considerable diversity in prognosis is seen with membranous nephropathy (MN). In terms of pathological findings, the presence of tubulointerstitial lesions was emphasized as a poor prognostic factor. However, the glomerular factors affecting the long-term outcome of idiopathic human MN have remained unclear. We reviewed the initial clinicopathological factors affecting the primary and secondary outcomes in 105 patients with primary MN, as well as reviewing previous reports. Based on electron microscopic (EM) findings, we could divide patients into two subtypes and one subgroup; i.e., homogeneous type with a synchronous phase of electron-dense deposits, with large dense deposits (deep subgroup) and heterogeneous type with various phases of dense deposits. The homogeneous type showed no endstage renal failure, and had earlier remission as compared with the heterogeneous type. For the secondary outcome, heterogeneous type and deep subgroup were also independent risk factors. However, there was no significant difference in the final primary or secondary outcome for any treatment subgroups. These results indicated that our category of EM findings was a beneficial marker of the primary and secondary outcomes in MN; the homogeneous type of MN with synchronous phase of electron-dense deposits (except for the "deep" subgroup) had a good outcome. 相似文献