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91.
Levecke B Rinaldi L Charlier J Maurelli MP Morgoglione ME Vercruysse J Cringoli G 《Parasitology research》2011,109(3):953-957
The faecal egg count reduction test (FECR) is the recommended technique to monitor anthelmintic drug efficacy in livestock.
However, results are often inconclusive due to the low analytic sensitivity of the diagnostic technique or the conflict in
results from FECR formulae. A novel experimental set-up was, therefore, used to compare the impact of analytic sensitivity
and formulae on FECR results. Four McMaster techniques (analytic sensitivities 50, 33.3, 15 and 10) and a FLOTAC technique
(analytic sensitivity ~ 1) were used on faecal samples of 30 calves with a FEC of less than 200 eggs per gram. True drug efficacies
of 70%, 80% and 90% were experimentally mimicked by comparing FEC before and after dilution (3:10, 2:10 and 1:10, respectively).
The FECR was summarized using group (FECR(1)) and individual (FECR(2)) based formulae. There was a significant increase in
precision of FECR when the analytic sensitivity increased (p < 0.0001). The precision also depended on the formula used, FECR(1) (p < 0.05) resulting in more precise FECR compared to FECR(2). The accuracy of the FECR differed marginally between the two
formulae (p = 0.06), FECR(1) being more accurate. In conclusion, the present study describes a novel methodology to compare techniques
for the precision and the accuracy of their FECR results. The results underscored that techniques with high analytic sensitivity
will improve the interpretation of FECR in animal populations where baseline FEC are low. They also point out that the precision
of individual-based formulae is affected by the analytic sensitivity. 相似文献
92.
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95.
Robaeys G De Bie J Van Ranst M Buntinx F 《World journal of gastroenterology : WJG》2007,13(16):2379-2380
During treatment of chronic hepatitis C patients with interferon and ribavirin, a lot of side effects are described. Twenty-three percent to 44% of patients develop depression. A minority of patients evolve to psychosis. To the best of our knowledge, no cases of psychogenic parasitosis occurring during interferon therapy have been described in the literature. We present a 49-year-old woman who developed a delusional parasitosis during treatment with pegylated interferon alpha-2b weekly and ribavirin. She complained of seeing parasites and the larvae of fleas in her stools. This could not be confirmed by any technical examination. All the complaints disappeared after stopping pegylated interferon alpha-2b and reappeared after restarting it. She had a complete sustained viral response. 相似文献
96.
This naturalistic study aims to evaluate the influence of antipsychotic treatment on the use of anticholinergics. The observed use of anticholinergics will give an indication of the occurrence of extrapyramidal side effects (EPS) in the different antipsychotic treatment conditions. The medication use of 1215 hospitalised patients with DSM-IV 295.xx diagnosis is recorded. Four antipsychotic treatment conditions are distinguished: 1) only first generation antipsychotics (FGA): patients receive one or a combination of first generation antipsychotics, 2) a combination of high potency FGA and second generation antipsychotics (SGA), 3) a combination of low potency FGA and SGA, and 4) only SGA: patients receive one or a combination of SGA. Antipsychotic treatment significantly influences the use of anticholinergics. Anticholinergic use is highest in patients treated with high potency FGA (whether or not in combination with SGA) as compared with patients only treated with SGA and patients combining SGA with low potency FGA. The two latter groups do not significantly differ. However, there were no significant differences in the prevalence of EPS with the exception of akathisia between FGA and SGA. Thus, through the use of anticholinergics, EPS induced by FGA can be effectively reduced. 相似文献
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98.
Violetta Sulzyc-Bielicka Pawel Domagala Ewa Majdanik Maria Chosia Dariusz Bielicki Jozef Kladny Mariusz Kaczmarczyk Krzysztof Safranow Wenancjusz Domagala 《Virchows Archiv : an international journal of pathology》2009,454(6):695-702
Colorectal carcinoma (CRC) is a heterogeneous disease with specific epidemiological, pathological, molecular, and clinical
characteristics that depend on the location of the tumor relative to the splenic flexure. Thymidylate synthase (TS) is a major
target of 5-fluorouracil-based chemotherapy for CRC and high expression of this enzyme in tumor cells can influence the effect
of therapy. We examined differences in TS protein expression in nuclei of tumor cells between CRCs located proximal and distal
to the splenic flexure. Nuclear TS was detected by immunohistochemistry with a TS 106 monoclonal antibody on tissue microarrays
constructed from 269 CRCs. The median histological score of nuclear TS expression of all proximal tumors was two times higher
(p = 0.0003) and in men three times higher (p = 0.00023) than that found in distal tumors. In multivariate analysis which included age, sex, Astler–Coller stage, histological
grade, and site, only proximal location of the tumor was identified as an independent factor associated with higher TS expression
(odds ratio 2.46, 95% confidence interval = 1.29–4.70, p = 0.0062). These results demonstrate significant differences in nuclear TS expression between proximal and distal cancers
and suggest the potential importance of the site of the tumor for proper stratification of patients for chemotherapy. 相似文献
99.
Jana Vranova Stepan Vinakurau Jan Richter Miroslav Starec Anna Fiserova Jozef Rosina 《Radiation oncology (London, England)》2011,6(1):1-13
Background
The protein kinase CK2 sustains multiple pro-survival functions in cellular DNA damage response and its level is tightly regulated in normal cells but elevated in cancers. Because CK2 is thus considered as potential therapeutic target, DNA double-strand break (DSB) formation and rejoining, apoptosis induction and clonogenic survival was assessed in irradiated mammalian cells upon chemical inhibition of CK2.Methods
MRC5 human fibroblasts and WIDR human colon carcinoma cells were incubated with highly specific CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB), or mock-treated, 2 hours prior to irradiation. DSB was measured by pulsed-field electrophoresis (PFGE) as well as gamma-H2AX foci formation and removal. Apoptosis induction was tested by DAPI staining and sub-G1 flow cytometry, survival was quantified by clonogenic assay.Results
TBB treatment did not affect initial DNA fragmention (PFGE; up to 80 Gy) or foci formation (1 Gy). While DNA fragment rejoining (PFGE) was not inhibited by the drug, TBB clearly delayed gamma-H2AX foci disappearence during postirradiation incubation. No apoptosis induction could be detected for up to 38 hours for both cell lines and exposure conditions (monotherapies or combination), but TBB treatment at this moderately toxic concentration of 20 μM (about 40% survival) enhanced radiation-induced cell killing in the clonogenic assay.Conclusions
The data imply a role of CK2 in gamma-H2AX dephosporylation, most likely through its known ability to stimulate PP2A phosphatase, rather than DSB rejoining. The slight but definite clonogenic radiosensitization by TBB does apparently not result from interference with an apoptosis suppression function of CK2 in these cells but could reflect inhibitor-induced uncoupling of DNA damage response decay from break ligation. 相似文献100.
Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation 总被引:1,自引:0,他引:1
Moran-Crusio K Reavie L Shih A Abdel-Wahab O Ndiaye-Lobry D Lobry C Figueroa ME Vasanthakumar A Patel J Zhao X Perna F Pandey S Madzo J Song C Dai Q He C Ibrahim S Beran M Zavadil J Nimer SD Melnick A Godley LA Aifantis I Levine RL 《Cancer cell》2011,20(1):11-24
Somatic loss-of-function mutations in the ten-eleven translocation 2 (TET2) gene occur in a significant proportion of patients with myeloid malignancies. Although there are extensive genetic data implicating TET2 mutations in myeloid transformation, the consequences of Tet2 loss in hematopoietic development have not been delineated. We report here an animal model of conditional Tet2 loss in the hematopoietic compartment that leads to increased stem cell self-renewal in vivo as assessed by competitive transplant assays. Tet2 loss leads to a progressive enlargement of the hematopoietic stem cell compartment and eventual myeloproliferation in vivo, including splenomegaly, monocytosis, and extramedullary hematopoiesis. In addition, Tet2(+/-) mice also displayed increased stem cell self-renewal and extramedullary hematopoiesis, suggesting that Tet2 haploinsufficiency contributes to hematopoietic transformation in vivo. 相似文献