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Protein C has been purified from the plasma of a patient with thrombotic diathesis. Both before and after isolation, the protein showed reduced capacity to hydrolyze synthetic substrates and to anticoagulate plasma. Proteolysis with the soluble thrombin- thrombomodulin complex proceeded normally and to completion as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Approximately one-third of the protein is functional, indicating a heterozygous defect. Indirect studies suggest that the abnormal component can bind to protein S and phospholipids. Both forms of activated protein C can also incorporate radiolabeled diisopropylfluorophosphate. 相似文献
93.
In anesthetized cats, reflex deglutition elicited by electrical stimulation of the superior laryngeal nerve was facilitated by concurrent stimulation of points along a classical projection extending from the orbital gyrus to the pons, as well as by activation of diencephalic and midbrain ventral tegmental sites outside this corticobulbar trajectory. Extensive damage to the corticifugal projection had no effect on facilitation induced by diencephalic and mesencephalic stimulation, whereas reflex facilitation was either diminished or abolished by chronic diencephalic lesions which encroached upon the medial and rostral components of the ventral amygdalo-hypothalamic projection system. Supportive evidence suggests that facilitation of swallowing may also involve ventromedial tegmental dopamine neuron projections to the basal forebrain. 相似文献
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In anesthetized cats, swallowing elicited by electrical stimulation of the superior laryngeal nerves (SLNs) was inhibited by the GABA-mimetic muscimol and by diazepam, an action that was reversed by picrotoxin and bicuculline. This inhibition supports the involvement of GABA receptors, specifically those of the GABAA subtype which both antagonists have been shown to block in various areas of the central nervous system. The inhibition of reflex swallowing and its reversal were unaltered by a transection of the brainstem at a midcollicular level. Stimulation of the SLNs also caused a bradycardia that was inhibited by both muscimol and diazepam and was restored by both GABA antagonists. Data from these experiments provide suggestive evidence for a role of GABA-ergic transmission in the central control of the deglutitory reflex. 相似文献
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Localization of pancreatic insulinoma: comparison of pre- and intraoperative US with CT and angiography 总被引:2,自引:0,他引:2
Galiber AK; Reading CC; Charboneau JW; Sheedy PF d; James EM; Gorman B; Grant CS; van Heerden JA; Telander RL 《Radiology》1988,166(2):405-408
Methods of preoperative radiologic localization of insulinoma were compared in 52 patients, 44 of whom had solitary tumors. Examinations performed in these 44 patients were preoperative ultrasonography (US) in 28, angiography in 26, and computed tomography in 23. Prospective sensitivities were 61%, 54%, and 30%, respectively. Imaging sensitivities were lower for the eight patients with multiple insulinomas. In 28 of the 44 patients, intraoperative US was performed without the examiner being aware of the surgical findings. The sensitivity was 84%. Four insulinomas were not palpable but were visualized sonographically. The combined sensitivity of intraoperative US and surgical palpation for detecting solitary insulinomas was 100%. High-frequency intraoperative US is valuable for detecting occult solitary insulinomas and considerably useful for determining the proximity of insulinomas to the pancreatic and bile ducts. 相似文献
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