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排序方式: 共有661条查询结果,搜索用时 15 毫秒
61.
Masakazu Ichinose Hisamichi Aizawa Akitoshi Ishizaka Atsushi Nagai Yoshinosuke Fukuchi Hana Mullerova Martin Crane 《Nihon Kokyūki Gakkai zasshi》2007,45(12):927-935
To grasp the burden and management status of COPD in Japan, a large telephone survey was conducted. In initial screening 400 individuals > or =45 years were identified as either having been given a diagnosis of COPD or fulfilling criteria for their respiratory-related symptoms and smoking history (baseline population) and they were recruited for a detailed investigation (interview sample). They were asked about demographic information, exacerbation, impact of COPD on daily life, and management and treatment. Of the 400 interview samples, 209 subjects (52%) had a diagnosis of COPD, and the remaining 191 ones (48%) were not, retrospectively. It was confirmed that proportions of a current smoker in the COPD (35.4%) and non-COPD (35.6%) groups were almost at the same level. The use of inhaled bronchodilators, recommended by guidelines in 157 treated subjects, was 16% or less, whereas respiratory conditions affected daily activities in 70% of all the subjects. In conclusion, COPD in Japanese subjects significantly affects daily life yet is undiagnosed; there is a need to improve COPD diagnosis and management by general practitioners through disseminating guidelines for diagnosis, management, and prevention of COPD in Japan. 相似文献
62.
Seiichi Okabe Tetsuzo Tauchi Yuko Tanaka Juri Sakuta Kazuma Ohyashiki 《Oncotarget》2015,6(24):20231-20240
The potency of Abelson (ABL) tyrosine kinase inhibitors (TKIs) against chronic myeloid leukemia (CML) has been demonstrated. However, ABL TKI resistance can develop. In this study, we investigated the efficacy of a combination therapy including rigosertib (ON 01910.Na), a polo-like kinase (PLK) and phosphoinositide 3-kinase (PI3K) inhibitor, and ABL TKIs. A 72-h rigosertib treatment was found to inhibit cell growth, induce apoptosis, reduce phosphorylation of the breakpoint cluster region-c (BCR)-ABL and its substrate Crk-L, and increase the activities of caspase 3 and poly (ADP-ribose) polymerase (PARP). This combination therapy also exerted a synergistic inhibitory effect on Philadelphia chromosome (Ph)-positive cell proliferation and reduced the phosphorylation of BCR-ABL and Crk-L while increasing that of cleaved PARP and the H2A.X histone. Rigosertib also potently inhibited the growth of ABL TKI-resistant cells, and cotreatment with ABL TKIs and rigosertib induced higher cytotoxicity. These results indicate that rigosertib treatment may be a powerful strategy against ABL TKI-resistant cells and could enhance the cytotoxic effects of ABL TKIs. 相似文献
63.
64.
Kizaki M Okamoto S Tauchi T Tanaka H Tanimoto M Inokuchi K Murayama T Saburi Y Hino M Tsudo M Shimomura T Isobe Y;TARGET Investigators 《International journal of hematology》2008,88(4):409-417
Since hematology is a highly specialized field, an individual physician may not have much direct treatment experience with
a given disease. Therefore, the Japanese Society of Hematology (JSH) has discussed establishing a registry of hematologic
disorders, in order to contribute to improving the quality of treatments and clinical outcomes in this field. As a first step,
the Timely and Appropriate Registration System for GLIVEC Therapy (TARGET), a registration system for chronic myeloid leukemia
(CML) patients treated with imatinib, was established in October 2003. We present the preliminary results of the first 4 years’
experience with this system. CML patients treated with imatinib in Japan were registered through the website and the patient’s
clinical course, including parameters and events like imatinib dose, blood counts, adverse events, and efficacy were recorded
in months 1, 3, 6, 9, and 12 and then every 6 months thereafter. By September 2007, 862 patients from 176 hospitals were registered.
Follow-up period was 0–54 months, and 127 patients were followed-up for more than 36 months. Based on these cumulative data,
present imatinib treatment trends were analyzed and safety and efficacy were compared with international trial data.
The part of content in this article was presented in the Joint Annual Conference of the 68th Japanese Society of Hematology
(JSH) and the 48th Japanese Society of Clinical Hematology (JSCH) in Fukuoka (Japan) at 7 October 2006.
Other participants in the TARGET system are listed in the Appendix. 相似文献
65.
Role of p21 RAS in p210 bcr-abl transformation of murine myeloid cells 总被引:11,自引:2,他引:11
Mandanas RA; Leibowitz DS; Gharehbaghi K; Tauchi T; Burgess GS; Miyazawa K; Jayaram HN; Boswell HS 《Blood》1993,82(6):1838-1847
The p21 RAS product has been implicated as part of the downstream signaling of certain nonreceptor tyrosine kinase oncogenes and several growth factor receptor-ligand interactions. We have reported that the chronic myelogenous leukemia oncogene p210 bcr-abl transforms a growth- factor-dependent myeloid cell line NFS/N1.H7 to interleukin-3 (IL-3) independence. In these p210 bcr-abl-transformed cells (H7 bcr-abl.A54) and in two other murine myeloid cell lines transformed to IL-3 independence by p210 bcr-abl, endogenous p21 RAS is activated as determined by an elevated ratio of associated guanosine triphosphate (GTP)/guanosine diphosphate (GDP), assayed by thin-layer chromatography of the nucleotides eluted from p21 RAS after immunoprecipitation with the Y13-259 antibody. Treatment of p210 bcr-abl-transformed cells with a specific tyrosine kinase inhibitor herbimycin A resulted in diminished tyrosine phosphorylation of p210 bcr-abl and associated proteins, without major reduction in expression of the p210 bcr-abl protein itself. Inhibition of p210 bcr-abl-dependent tyrosine phosphorylation resulted in a reduction of active p21RAS-GTP complexes in the transformed cells, in diminished expression of the nuclear early response genes c-jun and c-fos, and in lower cellular proliferation rate. To further implicate p21 RAS in these functional events downstream of p210 bcr-abl tyrosine phosphorylation, we targeted G- protein function directly by limiting the availability of GTP with the inosine monophosphate dehydrogenase inhibitor, tiazofurin (TR). In p210 bcr-abl-transformed cells treated for 4 hours with TR, in which the levels of GTP were reduced by 50%, but GDP, guanosine monophosphate, and adenosine triphosphate (ATP) were unaffected, p210 bcr-abl tyrosine phosphorylation was at control levels. However, expression of c-fos and c-jun nuclear proto-oncogenes were strongly inhibited and p21 RAS activity was downregulated. These findings show that p210 bcr-abl transduces proliferative signals, in part, through downstream activation of p21 RAS. Furthermore, p21 RAS activity is linked to pathways that regulate c-jun and c-fos expression. 相似文献
66.
Male Donryu rats were housed in a specific pathogen free room and fed with 60% of the mean daily quantity of diet consumed by rats fed ad libitum The animals were divided into three groups of 50 and each group fed different amounts and types of dietary protein. The group P40 was fed with a diet containing 40% of total volume as vegetable protein; the agroup P10 with 10% of total volume as vegetable protein; and the group CE7 with 18% of total volume as mixed vegetable and animal proteins. Subsets of the animals were sacrificed at the ages of 6, 12, 18, 24 and 30 months. The P40 group gained body weight faster than other two groups during the first months. However, after 12 months, the body weight began to decrease, reaching the lowest mean body weight among the three groups by the end of the study. The three groups did not show any significative difference in the muscle fiber number index and in the number of capillaries per muscle fiber. The calculated diaphragm volume index increased up to a peak at 12 months in all three groups decreasing gradually thereafter, but did not show any significant difference due to dietary protein intake. The data suggest that the morphological age changes occurring in the diaphragm muscle are likely unrelated to dietary conditions and are more likely due to other factors such as changes in the neural activity during aging. 相似文献
67.
Matsubara M Ohkubo T Michimata M Hozawa A Ishikawa K Katsuya T Nagai K Tsuji I Higaki J Araki T Satoh H Hisamichi S Ito S Ogihara T Imai Y 《Journal of hypertension》2000,18(7):861-866
OBJECTIVE: To assess the implications of polymorphisms of the amiloride-sensitive epithelial sodium channel in essential hypertension in the Japanese population by determining the incidence of the T594M mutation in the , subunit of the epithelial sodium channel, and by screening the C-terminus of the epithelial sodium channel. METHODS: Single-strand confirmational polymorphism (SSCP) analysis using two sets of primers which cover the last two-thirds of the last exon coding the B epithelial sodium channel and modification of a specific enzyme restriction site (NlaIII) for the T594M mutation were performed on 803 Japanese subjects. They were randomly selected from the study participants representative of a general population of Ohasama, Japan, who measured their home blood pressure. Polymerase chain reaction (PCR) products presenting a shift in SSCP gel, as well as controls, were directly sequenced by autoanalyser to identify the mutation. RESULTS: SSCP analysis identified altered migration in five subjects. Four SSCP variants found by sequencing were heterogeneous for the P592S (CCT to TCT) mutation conserving the PY motif, although it was not significantly associated with either home or casual blood pressure values. The resting polymorphism was at codon Thr 594, leading to no change in the amino acid sequence (ACG to ACA). None of the PCR products were modified by NlaIII, indicating the absence of the T594M mutation. CONCLUSIONS: The epithelial sodium channel variants at the C-terminus are not involved in the common form of essential hypertension in Japanese. 相似文献
68.
69.
Ueno K Kawayama T Edakuni N Koga T Aizawa H 《Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases》2006,80(5):527-530
A 63-year-old woman suffering from dyspnea since 1 week earlier underwent a combination therapy of oral corticosteroid and ciclosporin for rheumatic arthritis. Chest radiography showed plural effusion with gas formation in the right thorax. Empyema was diagnosed based on the specimen from pleural effusion. After a chest tube was emplaced to remove and wash out pus, antibiotics were started and empyema improved immediately. Streptococcus dysgalactiae subsp. equisimilis, but not aerobic bacilli, was detected from the pus by repeated culture. Insofar as we know this is the first case of empyema with gas formation associated with Streptococcus dysgalactiae subsp. equisimilis. 相似文献
70.
Morio T Atsuta Y Tomizawa D Nagamura-Inoue T Kato K Ariga T Kawa K Koike K Tauchi H Kajiwara M Hara T Kato S;Japanese Cord Blood Bank Network 《British journal of haematology》2011,154(3):363-372
We report the results of umbilical cord blood transplantation (UCBT) performed in 88 patients with primary immunodeficiency (PID) between 1998 and 2008 in Japan; severe combined immunodeficiency (SCID, n = 40), Wiskott-Aldrich syndrome (WAS, n = 23), chronic granulomatous disease (n = 7), severe congenital neutropaenia (SCN, n = 5) and other immunodeficiencies (n = 13). Five-year overall survival (5-year OS) for all patients was 69% [95% confidence interval (CI), 57-78%], and was 71% and 82% for SCID and WAS, respectively. The main cause of death before day 100 was infection (17/19), while that after day 100 was graft-versus-host disease (GVHD) (5/7). Using multivariate analyses, pre-transplant infection, no conditioning, ≥ 2 human leucocyte antigen (HLA) mismatches or diagnosis other than SCID, SCN or WAS were all associated with poor prognosis. Reduced-intensity conditioning was associated with decreased overall mortality compared with myeloablative therapy. The cumulative incidence of grade 2-4 acute GVHD at day 100 was 28% (95% CI, 19-38%), and that of chronic GVHD at day 180 was 13% (95% CI, 7-23%). We conclude that UCBT should be considered for PID patients without an HLA-matched sibling. The control of pre-transplant infection and selection of HLA-matched donors will lead to a better outcome. 相似文献