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排序方式: 共有442条查询结果,搜索用时 15 毫秒
81.
Hanane Chemlal Sihem Bensalem Keltouma Bendiab Meriem Azzar Amine Benberkane Kamel Lalaoui Mokrane Iguer-Ouada Lamine Bournine 《Andrologia》2021,53(1):e13902
The aim of this study is to investigate, by a validated in vitro model, the effect of diabetic plasma on ejaculated human spermatozoa. Plasma of 51 male diabetic patients (mean age 62.28 ± 9.28 years) was selected according to their HbA1c level: low HBA1c ≤ 5% (31 mmol/mol), moderate HBA1c 6%–8% (42–64 mmol/mol) and high HBA1c ≥ 10% (86 mmol/mol). The plasma was tested on eighteen normal semen samples by analysing gametes motility using a computer Sperm Class Analyzer® and their corresponding oxidative stress (OS) status using thiobarbituric acid-reactive substances assay. The results indicated that diabetic plasma affected all sperm motility parameters with high HbA1c showing the most important deleterious effects. Low gametes' straight-line velocity was observed in high HbA1c level, mainly after 20 min of co-incubation (8.78 ± 0.47 µm/s). Also, the highest lipid peroxidation (nmoles MDA/108 SPZ) was observed in high HbA1c values (0.92 ± 0.09), higher than those in spermatozoa treated with H2O2 (0.85 ± 0.04). Conclusively, a direct impact of diabetic plasma on spermatozoa is revealed with overexpression of OS as the underlying mechanism. These findings suggested that it is strongly recommended to control clinically the glycaemic level and OS in diabetic patients for the maintenance of male fertility. 相似文献
82.
Lindsay B. Alcaraz Aude Mallavialle Caroline Mollevi Florence Boissière-Michot Hanane Mansouri Joelle Simony-Lafontaine Valérie Laurent-Matha Thierry Chardès William Jacot Andrei Turtoi Pascal Roger Séverine Guiu Emmanuelle Liaudet-Coopman 《International journal of cancer. Journal international du cancer》2023,152(6):1243-1258
Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and lacks specific targeted therapeutic agents. The current mechanistic evidence from cell-based studies suggests that the matricellular protein SPARC has a tumor-promoting role in TNBC; however, data on the clinical relevance of SPARC expression/secretion by tumor and stromal cells in TNBC are limited. Here, we analyzed by immunohistochemistry the prognostic value of tumor and stromal cell SPARC expression in 148 patients with non-metastatic TNBC and long follow-up (median: 5.4 years). We also quantified PD-L1 and PD-1 expression. We detected SPARC expression in tumor cells (42.4%), cancer-associated fibroblasts (CAFs; 88.1%), tumor-associated macrophages (77.1%), endothelial cells (75.2%) and tumor-infiltrating lymphocytes (9.8%). Recurrence-free survival was significantly lower in patients with SPARC-expressing CAFs. Multivariate analysis showed that SPARC expression in CAFs was an independent prognostic factor. We also detected tumor and stromal cell SPARC expression in TNBC cytosols, and in patient-derived xenografts and cell lines. Furthermore, we analyzed publicly available single-cell mRNA sequencing data and found that in TNBC, SPARC is expressed by different CAF subpopulations, including myofibroblasts and inflammatory fibroblasts that are involved in tumor-related processes. We then showed that fibroblast-secreted SPARC had a tumor-promoting role by inhibiting TNBC cell adhesion and stimulating their motility and invasiveness. Overall, our study demonstrates that SPARC expression in CAFs is an independent prognostic marker of poor outcome in TNBC. Patients with SPARC-expressing CAFs could be eligible for anti-SPARC targeted therapy. 相似文献
83.
New cytotoxic aristolactams from Pararistolochia flos-avis 总被引:1,自引:0,他引:1
Two new aristolactams, aristolactam-FI [1] and -FII [2], were isolated from active extracts of Pararistolochia flos-avis. Their structures were elucidated on the basis of nmr, ms, uv, and ir spectral data. Aristolactam-I [3] and -AII [4] were also isolated from this plant. Aristolactam-AII showed cytotoxicity against PS and KB cells in culture. 相似文献
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85.
Ezh2 and Runx1 Mutations Collaborate to Initiate Lympho-Myeloid Leukemia in Early Thymic Progenitors
Christopher A.G. Booth Nikolaos Barkas Wen Hao Neo Hanane Boukarabila Elizabeth J. Soilleux George Giotopoulos Noushin Farnoud Alice Giustacchini Neil Ashley Joana Carrelha Lauren Jamieson Deborah Atkinson Tiphaine Bouriez-Jones Rab K. Prinjha Thomas A. Milne David T. Teachey Elli Papaemmanuil Brian J.P. Huntly Adam J. Mead 《Cancer cell》2018,33(2):274-291.e8
86.
Are HLA antigens implicated in the pathogenesis of non-haemorrhagic deterioration following aneurysmal subarachnoid haemorrhage. 下载免费PDF全文
R H Lye P A Dyer S Sheldon N Antoun 《Journal of neurology, neurosurgery, and psychiatry》1989,52(10):1197-1199
Aneursymal subarachnoid haemorrhage is frequently complicated by non-haemorrhagic deterioration. HLA typing was studied in a group of 40 patients who had sustained an aneurysmal subarachnoid haemorrhage in order to assess its ability to identify those who are likely to experience non-haemorrhagic deterioration. The antigen HLA-B7 appeared to be associated with an increased likelihood of this event supervening. Conversely, the antigen HLA-DR3, seemed to exert a "protective" influence. 相似文献
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The combination of GM-CSF and IL-2 as local adjuvant shows synergy in enhancing peptide vaccines and provides long term tumor protection 总被引:1,自引:0,他引:1
Toubaji A Hill S Terabe M Qian J Floyd T Simpson RM Berzofsky JA Khleif SN 《Vaccine》2007,25(31):5882-5891
Many strategies have been used to enhance the peptide vaccine immune response and to establish therapeutic benefits. This includes the utilization of cytokines to improve antigen presentation or enhance T cell response. Here, we have tested the combination of GM-CSF and IL-2 as locally administered adjuvant to enhance the immune response to the HPV16 E7 peptide. Female C57BL/6 mice were immunized intradermally with a 9-mer HPV16 E7 peptide (aa: 49-57) alone, or in combination with GM-CSF, IL-2, or both cytokines. Specific immune responses were measured by ELISA and Chromium-Release Assays. Furthermore, therapeutic effects of these vaccines and long term tumor protection were assessed in mice bearing established tumors. We showed that GM-CSF and IL-2, when co-administered locally in an emulsion with peptide, exert a synergistic effect in enhancing the immune response to the antigen. This combination induced higher CTL and cytokine release responses and did not increase the T(reg) population. Therapeutic intervention with this synergistic combination led to a complete response of established tumors. Furthermore, this combination induced a memory response which protected mice against subsequent additional tumor challenge. We identified a new vaccine adjuvant, a local combination of GM-CSF and IL-2, which is synergistic in enhancing peptide specific immune response through local effect without increasing T(reg) cells. This immune response was found to be long lasting and protective in tumor bearing mice. 相似文献