全文获取类型
收费全文 | 1182篇 |
免费 | 41篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 150篇 |
妇产科学 | 8篇 |
基础医学 | 82篇 |
口腔科学 | 7篇 |
临床医学 | 119篇 |
内科学 | 329篇 |
皮肤病学 | 25篇 |
神经病学 | 81篇 |
外科学 | 200篇 |
综合类 | 5篇 |
预防医学 | 11篇 |
眼科学 | 2篇 |
药学 | 113篇 |
中国医学 | 5篇 |
肿瘤学 | 84篇 |
出版年
2017年 | 7篇 |
2016年 | 14篇 |
2015年 | 23篇 |
2014年 | 19篇 |
2013年 | 18篇 |
2012年 | 12篇 |
2011年 | 9篇 |
2010年 | 22篇 |
2009年 | 30篇 |
2008年 | 7篇 |
2007年 | 37篇 |
2006年 | 63篇 |
2005年 | 34篇 |
2004年 | 42篇 |
2003年 | 37篇 |
2002年 | 15篇 |
2001年 | 12篇 |
2000年 | 6篇 |
1999年 | 42篇 |
1998年 | 84篇 |
1997年 | 77篇 |
1996年 | 73篇 |
1995年 | 64篇 |
1994年 | 59篇 |
1993年 | 49篇 |
1992年 | 21篇 |
1991年 | 23篇 |
1990年 | 21篇 |
1989年 | 14篇 |
1988年 | 12篇 |
1987年 | 12篇 |
1986年 | 14篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 15篇 |
1980年 | 11篇 |
1979年 | 15篇 |
1978年 | 6篇 |
1976年 | 6篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1970年 | 4篇 |
1959年 | 8篇 |
1958年 | 33篇 |
1957年 | 22篇 |
1956年 | 21篇 |
1955年 | 19篇 |
1954年 | 25篇 |
排序方式: 共有1226条查询结果,搜索用时 15 毫秒
31.
H. NIIKURA H. YOSHIDA K. ITO T. TAKANO H. WATANABE† S. AIBA† & N. YAEGASHI 《International journal of gynecological cancer》2006,16(3):1212-1215
The aim of this study was to evaluate clinicopathologic characteristics of primary cutaneous Paget's disease of the vulva. Between 1986 and 2005, 22 patients with primary cutaneous Paget's disease of the vulva (type 1) were treated at Tohoku University Hospital. Medical records were reviewed for pathologic diagnosis, patient age, associated neoplasms, type(s) of eczema, symptom duration, treatment, surgical procedures, recurrence, and length of follow-up. Patient age ranged from 51 to 85 years (median 71.5 years). Median duration of symptoms was 24 months (range 2-60 months). Type 1a (intraepithelial) Paget's disease accounted for 18 patients, with 3 type 1b (invasive) cases and 1 type 1c (intraepithelial disease with underlying adenocarcinoma) case. Mean length of follow-up was 53.7 months, and median follow-up was 49 months (range 6-199 months). Only two patients had an associated internal malignancy: T-cell leukemia and breast cancer. Mapping biopsy was performed in 14 of the 18 type 1a cases. All patients were free of disease at the surgical margins and are alive without recurrence. The four patients with type 1b or 1c disease had lymph node metastases. Two has died of disease, and two are alive with no recurrence. The rate of secondary malignancy seems to be low in primary cutaneous Paget's disease of the vulva. Mapping biopsy with careful examination of characteristic skin surface may be useful for surgery of type 1a cases. Inguinal lymphadenectomy is recommended in cases with question of invasion or known underlying adenocarcinoma. 相似文献
32.
33.
34.
YASUKO HATANO SHOICHIRO TANIUCHI MIDORI MASUDA SHOJI TSUJI TAICHI ITO MASAFUMI HASUI YOHNOSUKE KOBAYASHI KAZUNARI KANEKO 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2009,117(2):115-123
Eosinophils are characterized by several functional properties, such as chemotaxis, adhesion, superoxide anion production, and degranulation. In this article, we have studied the role of bacterial ingestion by eosinophils in comparison with that by neutrophils. Eosinophils and neutrophils were purified by using the Percoll gradient method followed by selection with CD16‐coated immunomagnetic beads and centrifugation through a Ficoll‐Hypaque gradient combined with dextran sedimentation, respectively. Both cells were preincubated with anti‐FcγRIIa mAb (CD32 mAb), anti‐FcγRIIIb mAb (CD16 mAb), anti‐CR3 (CD11b mAb), or anti‐CR1 (CD35 mAb) before being examined for phagocytosis of opsonized heat‐killed Staphylococcus aureus (S. aureus). Phagocytosis and production of hydrogen peroxide were simultaneously measured by flow cytometry using S. aureus labeled with propidium iodide and stained with 2′,7′‐dichlorofluorescein diacetate. Eosinophils showed significantly lower activity than neutrophils in both phagocytosis and hydrogen peroxide production. Phagocytosis by both cells was decreased by heat‐inactivated serum. Phagocytosis by neutrophils was significantly inhibited by CD16 mAb and CD32 mAb, whereas that by eosinophils was only inhibited by CD35 mAb. Whereas the mechanism of phagocytosis by neutrophils was mediated by CD16 and CD32, that of eosinophils was modulated by complement receptor 1 (CD35). 相似文献
35.
TOMOYUKI TAHARA KENSUKE USUKI HIROAKI SATO HIDEYA OHASHI HARUHIKO MORITA HARUHIKO TSUMURA ATSUSHI MATSUMOTO HIROSHI MIYAZAKI AKIO URABE & TAKASHI KATO 《British journal of haematology》1996,93(4):783-788
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) has been established to estimate serum thrombopoietin (TPO) concentrations in healthy volunteers and patients with haemopoietic disorders. The ELISA uses a mouse monoclonal antibody (Ab) as the capture Ab and a biotinylated rabbit polyclonal Ab as the detector. The ELISA was reproducible, highly sensitive and specific for human TPO. The coefficients of intra- and inter-assay variation were from 3.0% to 4.9% and from 5.9% to 6.1%, respectively. The quantitative limit of the ELISA was 0.09 fmol/ml in serum. The quantitative limit was lower than the normal level. The dose–response curves of serum samples from healthy volunteers and patients with haemopoietic disorders were parallel to the standard curves. The ELISA did not cross-react with a variety of blood components and cytokines to produce false-positive results.
The serum TPO concentrations from 29 normal males and 21 females were 0.79 ± 0.35 and 0.70 ± 0.26 fmol/ml, respectively. Serum TPO levels in patients with aplastic anaemia (AA), acute lymphocytic leukaemia (ALL) and essential thrombocythaemia (ET) were measured using the ELISA. The serum TPO levels in the patients with ET ( n = 6, 2.80 ± 1.55 fmol/ml) were higher than the normal level. The patients with AA ( n = 7, 18.53 ± 12.37 fmol/ml) and ALL ( n = 5, 10.36 ± 5.57 fmol/ml) had significantly higher serum TPO levels than normal individuals. These results indicate that the ELISA specific to TPO should prove useful in measuring the TPO concentration in serum samples. 相似文献
The serum TPO concentrations from 29 normal males and 21 females were 0.79 ± 0.35 and 0.70 ± 0.26 fmol/ml, respectively. Serum TPO levels in patients with aplastic anaemia (AA), acute lymphocytic leukaemia (ALL) and essential thrombocythaemia (ET) were measured using the ELISA. The serum TPO levels in the patients with ET ( n = 6, 2.80 ± 1.55 fmol/ml) were higher than the normal level. The patients with AA ( n = 7, 18.53 ± 12.37 fmol/ml) and ALL ( n = 5, 10.36 ± 5.57 fmol/ml) had significantly higher serum TPO levels than normal individuals. These results indicate that the ELISA specific to TPO should prove useful in measuring the TPO concentration in serum samples. 相似文献
36.
KATSUMOTO KATO HIROSHI SANO NAOYUKI KATADA DAISAKU NISHIMURA MASAYUKI TAKEICHI MASAKI KANZAKI TETSUO HAYAKAWA YASUO KOYAMA 《Journal of gastroenterology and hepatology》1987,2(6):539-546
In order to clarify the characteristics of infectious mononucleosis hepatitis (IMH) in Japan, 20 cases with IMH treated at Kamo Hospital during the past 6 years (Group I) were analysed in comparison with cases of acute viral hepatitis, especially type A. The test for heterophil antibody was positive in only two cases. During the same period 209 cases were treated for acute viral hepatitis (type A: 77 cases = Group A; type B: 61 cases; type non-A, non-B: 71 cases). In Group I the common clinical symptoms and signs were headache, sore throat and lymph node swelling; jaundice was not as common as in Group A. GOT and GPT activities increased moderately in the acute stage, but they were significantly lower than those in Group A. LDH, AP, GGT and LAP activities were disproportionately higher to GPT activity in Group I. Liver biopsy in the convalescent stage showed that lipofuscin deposition and sinusoidal mononuclear cell infiltration were more prominent in Group I, while sinusoidal neutrocyte infiltration and focal necrosis at periportal areas were more common in Group A. Differential diagnosis of the two diseases could be made using these clinical features and histological findings. However, immunological differentiation is required for specific diagnosis because some features such as fever, prolonged elevation of thymol turbidity test, atypical lymphocytes in peripheral blod and predilection for young people were observed in both groups. Furthermore, the present study indicated that IMH is no longer rare and most cases do not demonstrate heterophil antibody in Japan. 相似文献
37.
Kiyoshi ITO Tatsuro AOYAMA Yoshinari MIYAOKA Tetsuyoshi HORIUCHI Kazuhiro HONGO 《Neurologia medico-chirurgica》2015,55(7):557-563
Cervical spinal neurinomas are benign tumors that arise from nerve roots. Based on their location, these tumors can also take the form of a dumbbell-shaped mass. Treatment strategies for these tumors have raised several controversial issues such as appropriate surgical indications and selection of surgical approaches for cervical dumbbell-shaped spinal neurinomas. In this report, we review previous literature and retrospectively analyze cervical spinal neurinoma cases that have been treated at our hospital. Surgical indications and approaches based on tumor location and severity are discussed in detail. Thus, with advances in neuroimaging and neurophysiological monitoring, we conclude that appropriate surgical approaches and intraoperative surgical manipulations should be chosen on a case-by-case basis. 相似文献
38.
Time‐Dependent Changes in QT Dynamics after Initiation and Termination of Paroxysmal Atrial Fibrillation 下载免费PDF全文
39.
ANEES THAJUDEEN M.D. WARREN M. JACKMAN M.D. BRIAN STEWART M.S. IVAN COKIC M.D. HIROSHI NAKAGAWA M.D. Ph.D. MICHAEL SHEHATA M.D. ALLEN M. AMORN M.D. AVINASH KALI M.S. EZH LIU M.D. DORON HARLEV M.Sc. NATHAN BENNETT M.Eng. ROHAN DHARMAKUMAR Ph.D. SUMEET S. CHUGH M.D. XUNZHANG WANG M.D. 《Pacing and clinical electrophysiology : PACE》2015,38(6):663-674
40.
YUKO UCHIMURA‐MAKITA M.D. YUKIKO NAKANO M.D. Ph.D. TAKEHITO TOKUYAMA M.D. MAI FUJIWARA M.D. YOSHIKAZU WATANABE M.D. AKINORI SAIRAKU M.D. HIROSHI KAWAZOE M.D. HIROYA MATSUMURA M.D. NOZOMU ODA M.D. HIROKI IKANAGA M.D. CHIKAAKI MOTODA M.D. KENTA KAJIHARA M.D. Ph.D. NOBORU ODA M.D. Ph.D. RICHARD L. VERRIER Ph.D. YASUKI KIHARA M.D. Ph.D. 《Journal of cardiovascular electrophysiology》2014,25(9):1021-1027