广州管圆线虫病的临床预后

目的 :探讨 1997年 10～ 11月间 ,温州市区暴发的广州管圆线虫病的临床预后。方法 :入选病例符合如下条件 :(1)于 1997年 9月中旬～ 11月中旬期间急性起病 ;(2 )头痛和 (或 )游移性躯干四肢皮肤抚摸痛 ;(3)周围血液或脑脊液中嗜酸性粒细胞增高和 (或 )广州管圆线虫虫体抗原抗体阳性 (EL ISA法 ) ;(4)病前一个月内有食用未熟的淡水螺肉或贝虾史 ;(5 )排除其它蠕虫移行症。采用统一设计的随访调查表 ,由专人对符合上述标准的 35例 ,通过电话和(或 )面访 ,进行为期 1.5年的随访。结果 :全组无死亡 ,无复发 ,无严重残疾或并发症 ,遗留症状轻微 ,以局限性的主观或客观根性感觉障碍为主。主观症状多于客观体征。早期神经根痛严重者 ,后遗根性感觉异常较多。结论 :该组病例远期预后良好 ,病程自限。病性较重者多数可遗留轻微局限性感觉异常或感觉减退 ,可能与广州管圆线虫蚴对脊髓后根神经髓鞘直接或间接损害以及虫体死亡后致局部肉芽肿形成有关     

天水饮治疗中风急性期疗效观察

目的 :观察中药天水饮治疗中风急性期的疗效。方法 :将 93例中风急性期患者随机分成 2组 ,2组一般治疗相同 ,治疗组 62例加用天水饮 ,病程 1周内每日 2剂 ,1周后每日 1剂。对照组 3 1例加用尼可林 0 .5 g静滴及 2周内加用 2 0 %甘露醇静滴 ,2周后加服步长新脑心通治疗。 2组治疗 3 0日后进行疗效比较。结果 :治疗组愈显率为 79.0 4 % ,病程 10日内见效率为 62 .90 % ;对照组愈显率为 5 1.61% ,病程 10日内见效率为3 8.71% ,2组比较均有显著性差异 ( χ2 =7.3 9,P<0 .0 1和 χ2 =4 .88,P<0 .0 5 )。结论 :天水饮可改善病变区脑循环 ,增强脑细胞活力 ,促进语言及肢体功能恢复 ,对中风急性期有良好的疗效     

高血压脑出血血肿周围组织血-脑屏障变化的动态观察

目的动态观察高血压脑出血血肿周围组织血-脑屏障的变化.方法对6例高血压脑出血患者实施立体定向血肿碎吸术,术中取血肿周围少许脑组织进行超微病理观察.结果血-脑屏障的损伤与出血时间的长短呈正比.脑出血后24h,可见星形胶质细胞肿胀,部分细胞崩解、坏死.毛细血管周围细胞足突肿胀,血-脑屏障损坏.72h,部分星形胶质细胞高度肿胀,细胞器溶解.毛细血管内皮细胞胞核增大,胞质突入管腔,内皮细胞间紧密连接消失.4～7d,星形细胞高度肿胀,胞质内充满水肿液,细胞器消失,细胞变性.毛细血管星形细胞足突明显肿胀,血管周围可见微小出血灶.结论血-脑屏障的变化与患者的预后有密切关系.     

Effect of atrial natriuretic factor on renal prostaglandin E2 release in the anaesthetized dog

Experiments were undertaken in two groups of barbiturate anaesthetized dogs to examine whether atrial natriuretic factor (ANF) exerts an effect on renal release of prostaglandin E₂ (PGE₂). In the first group, intravenous infusion of ANF (50 ng min^-1kg^-1body wt) reduced basal PGE₂ release from 4.4 ± 0.8 pmol min^-1to 1.8 ± 0.7 pmol min^-1. In the second group, intrarenal infusion of an α-adrenoceptor agonist, phenylephrine (2.5–6.75 μg min^-1), raised PGE₂ release from 2.7 ± 0.5 pmol min^-1to 7.5 ± 1.3 pmol min^-1. During continuous α₁-adrenergic stimulation, intravenous infusion of ANF (100 ng min^-1kg^-1body wt) reduced PGE₂ release to 3.5 ± 1.0 pmol min^-1. These results demonstrate that ANF reduces basal and α₁-adrenergic stimulated renal PGE₂ release.     

Anti‐infliximab antibody status and its relation to clinical response in psoriatic patients: A pilot study

Although the mechanisms underlying the loss of response to infliximab are not completely understood, the formation of antibodies to infliximab (ATI) are thought to play a role. The aim of this study was to investigate the presence of ATI in psoriatic patients and to evaluate its relationship to the clinical response. Fifteen patients with psoriasis were treated with infliximab (5 mg/kg) every 8 weeks after an initial three‐dose induction treatment. An enzyme linked immunosorbent assay kit was used for analyzing the presence of ATI in sera. Effectiveness assessments included the change in Psoriasis Area and Severity Index (PASI) compared with study entry. Five (33.3%) patients developed ATI. While 5.9 ± 3.2 infliximab infusions achieved a fall in the PASI score from a mean of 20.4 ± 8.3 to 5.3 ± 2.4 in ATI‐negative patients, these values changed from 23.3 ± 11 to 10 ± 4.9 after 9 ± 5.2 infusions in ATI‐positive patients. Our results suggested that ATI measured in psoriatic patients are of clinical importance. Therefore, monitoring for the induction of ATI and rescue strategies should be developed to avoid or to maintain a delay in ATI development.     

Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide

Backround/aimCyclophosphamide (CP) is a drug used for treatment of many malignant diseases. However, it can cause serious side effects such as hemorrhagic cystitis and male infertility. Hydrogen sulfide (H2S) is a gaseous mediator and is suggested to have antioxidant, antiinflammatory, and antiapoptotic effects. In this study, dose-dependent effects of H2S donor sodium hydrosulfide (NaHS) on cyclophosphamide-induced hemorrhagic cystitis and testicular dysfunction were investigated in rats.Material and methodsRats were divided into 5 groups (n = 8): control, CP, NaHS25 μmol/kg, NaHS50 μmol/kg, and NaHS100 μmol/ kg. After treatment for 7 days intraperitoneally (ip), a single ip dose of CP 200 mg/kg was given on the 8th day. Then, treatment was continued for 7 days. In bladder and testicular tissues, IL 6, IL 10, cGMP, NO, H2S, FSH, LH, and testosterone levels were measured by ELISA. Histopathological examination with H&E staining, as well as immunohistochemical staining for acrolein in bladder and caspase-3 and APAF-1 in testis were performed.ResultsNaHS prevented the increased IL 6 and IL 10 values induced by CP as well as prevented the decrease in cGMP values associated with CP. There was no significant change in FSH values, but the LH value, which increased with CP, decreased with 25, 50, and 100 μmol/kg NaHS. In contrast, testosterone decreased in the CP group and increased in the treatment groups. NaHS was effective in many biochemical and histopathological parameters at 25 and 50 μmol/kg doses, and this effect decreased at 100 μmol/kg dose.ConclusionH2S has a protective and therapeutic effect on hemorrhagic cystitis and testicular dysfunction induced by cyclophosphamide. It can be suggested that H2S is a promising molecule in facilitating cancer treatment.     

几丁糖预防肘关节粘连的临床研究

目的　研究医用几丁糖预防肘关节粘连的临床作用。方法　对 2 6例肘关节僵硬患者进行手术松解 ,其中 12例术后关节腔内涂布 2 %几丁糖 ,另 14例术后不用几丁糖作为对照组 ,观察术后肘关节伸屈活动度改善程度以评定疗效。结果　 2 6例均获随访 ,随访时间 8～ 5 1个月 ,平均 2 4个月。几丁糖组术后肘关节平均活动度恢复到 92 .9°± 2 0 .9°,比术前增加 5 5 .0°± 15 .9°;对照组术后肘关节平均活动度恢复到 75 .4°± 17.5°,比术前增加38.2°± 11.9°。几丁糖组术后肘关节伸屈活动度增值明显大于对照组 ,有显著性差异 (P<0 .0 1)。结论　医用几丁糖具有减轻或预防肘关节松解术后再粘连的形成。     

胰头癌手术范围对预后的影响

目的: 探讨胰头癌切除范围对预后的影响。方法: 对比分析95例胰头癌患者中44例行经典胰十二指肠切除及51例行扩大胰十二指肠切除的生存期、手术并发症和死亡率等。结果: 扩大切除组和经典切除组术后1、3、5年存活率分别为72.5%、45%、17.5%和47.73%、19.35%、6.45%,两组间比较差异有显著性,扩大切除组1、3、5年存活率较经典切除组明显提高(P<0.05);两组手术并发症和死亡率 分 别 为 29.41%、5.88%和25%、4.55%,二者比较差异无显著性。结论: 扩大切除能改善胰头癌患者的预后。     

对苯二甲酸中微量杂质HPLC分析条件的优化

用离子交换高效液相色谱法分析对苯二甲酸中的微量杂质，并对该方法进行了系统的优化。通过选择流动相pH值使缓冲溶液的浓度降低至0．13mol／L，分析周期缩短至10min以下。结果表明：该方法的工作曲线相关系数在0．9998以上，回收率为98．7％～102．2％，相对标准偏差小于3．6％。