六磷酸肌醇对人结肠腺癌LoVo细胞增殖分化影响

目的观察六磷酸肌醇(IP6)对人结肠腺癌细胞株LoVo体外增殖、分化的影响.方法直接细胞记数法、MTT法、平皿集落形成实验、肠型ALP比活性测定等方法观察LoVo细胞增殖、分化特性.结果与对照组相比,IP6处理组在6h后细胞增殖与集落形成能力呈现抑制状态,并呈时间、剂量依赖关系.分化标志酶ALP比活性增高.结论IP6对LoVo细胞具有细胞毒作用,能明显抑制细胞增殖、诱导细胞分化.     

芦荟多糖的抗肿瘤作用

本文从现代医学对肿瘤的认识出发,结合祖国医药研究的宝贵经验,对芦荟多糖的抗肿瘤作用进行了初步的理论研究,采用荧光偏振技术,通过测膜荧光偏振度和微粘度的变化,反映膜脂流动性的大小,研究库拉索芦荟和木立芦荟多糖对荷瘤小鼠红细胞免疫的影响。与阴性组相比,尤以木立芦荟低剂量组效果最佳。结果表明,芦荟多糖确实具有增强S180小鼠体内红细胞免疫及抗肿瘤作用。     

青霉素G钠生产中乙酸乙酯回收工艺的研究

目的：通过对乙酸乙酯回收方法的改进获得符合企控标准成品并提高收率。方法：采用连续精馏法和双塔精馏法。结果：成品乙酸乙酯含量控制在75％以上,水分在0．3％以下。结论：改进后方法提高了三相组分分离的回收率。     

7-氨基-3-(1-吡啶甲基)头孢烯酸(7-APCA)卤化物的合成路线

7-氨基-3-(1-吡啶甲基)头孢烯酸(7-APCA)卤化物是头孢他啶合成的重要中间体,笔者在查阅了大量的文献资料的基础上,综述了7-氨基-3-(1-吡啶甲基)头孢烯酸(7-APCA)卤化物合成方法的优点、缺点,同时阐述了笔者试验结果,以7-ACA为原料制备7-APCA卤化物的合成路线。     

青霉素发酵液鼓式过滤过程研究

目的：优化鼓式过滤工艺提高滤液效价。方法：通过调整鼓式过滤机顶洗水量,减少板框复滤滤液体积,提高滤液效价。结果：方法可行,应用此工艺可减少水量30％,提高了滤液效价。     

中成药中牛黄的检测研究

目的：探讨中药质量标准研究的有关问题。方法：分析牛黄药材的应用及含牛黄中成药质量准研究的现状。结果：针对牛黄类药材及含牛黄类药材的制剂检测存在的不足建议进行有针对的质量标准研究。结论：中成药质量标准的研究是一项与时俱进的研究,应制定出能更好的控制药品质量的标准。     

Influence of neurophysin residues 1–8 on the optical activity of neurophysin-peptide complexes Direct evidence that the 1–8 sequence alters the environment of bound peptide

Circular dichroism was used to compare the environment of peptides bound to native and des 1-8 neurophysin in order to further elucidate the role of the neurophysin 1-8 sequence in peptide-binding. A very large positive ellipticity (～6000 degcm²dmol^?1), shown earlier to be induced in tyrosine at position 2 of peptides bound to the native protein, was determined by the present study to be paralleled by similar induced changes in tyrosine at peptide position 1. Deletion of the neurophysin 1-8 sequence led to loss of half of the induced optical activity at peptide positions 1 and 2 and changes in binding-induced optical activity in the protein, the latter partially assignable to protein disulfides. In the rnononitrated native and des 1-8 proteins, the optical activity of neurophysin Tyr-49, a residue at the peptide-binding site, was reduced by 80% in complexes of the des 1-8 protein relative to those of the native protein. The results suggest a role for neurophysin Arg-8 in modulating the optical activity at the binding site by directly placing a charge proximal to the binding site and/or by altering binding site conformation. The data provide the first unambiguous evidence of a difference in the environment of bound peptide between the native and des 1-8 proteins.     

Liver transplantation in Langerhans' cell histiocytosis (histiocytosis X)

Two children with biopsy-proven LCH underwent successful hepatic transplantation for end-stage liver disease. These patients were thought not to have active LCH disease at the time of transplantation, although one had developed a new osteolytic lesion a few months before the operation and the other had suspicious osteolytic lesions at the time of transplantation. The histologic examination of the excised liver showed features consistent with primary sclerosing cholangitis. The two patients had an excellent recovery with no evidence of progression of LCH or recurrence of the underlying disease in the hepatic allograft at 1 and 3 years after organ transplantation.