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Huebner Lucas Schroeder Ines Kraft Eduard Gutmann Marcus Biebl Johanna Klamt Amrei Christin Frey Jana Warmbein Angelika Rathgeber Ivanka Eberl Inge Fischer Uli Scharf Christina Schaller Stefan J. Zoller Michael 《Der Anaesthesist》2022,71(10):795-800
Die Anaesthesiologie - Bei etwa 43?% aller Überlebenden der Intensivmedizin wird ein erworbenes Syndrom an Muskelschwäche beobachtet, welches Überleben und Lebensqualität... 相似文献
113.
Davide Carino MD Alejandro Fernández-Cisneros MD Marta Hernández-Meneses MD Elena Sandoval MD FECTS Jaume Llopis MD PhD Carlos Falces MD PhD José M. Miró MD PhD Eduard Quintana MD PhD FECTS Grup d'Estudi d'Endocarditis Infecciosa de l'Hospital Clínic de Barcelona 《Journal of cardiac surgery》2020,35(11):3034-3040
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Toward hyperpolarized molecular imaging of HIV: synthesis and longitudinal relaxation properties of 15N‐Azidothymidine
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Roman V. Shchepin Eduard Y. Chekmenev 《Journal of labelled compounds & radiopharmaceuticals》2014,57(10):621-624
Previously unreported 15N labeled Azidothymidine (AZT) was prepared as an equimolar mixture of two isotopomers: 1‐15N‐AZT and 3‐15N‐AZT. Polarization decay of 15N NMR signal was studied in high (9.4 T) and low (~50 mT) magnetic fields. 15N T1 values were 45 ± 5 s (1‐15N‐AZT) and 37 ± 2 s (3‐15N‐AZT) at 9.4 T, and 140 ± 16 s (3‐15N‐AZT) at 50 mT. 15N‐AZT can be potentially 15N hyperpolarized by several methods. These sufficiently long 15N‐AZT T1 values potentially enable hyperpolarized in vivo imaging of 15N‐AZT, because of the known favorable efficient (i.e., of the time scale shorter than the longest reported here 15N T1) kinetics of uptake of injected AZT. Therefore, 3‐15N‐AZT can be potentially used for HIV molecular imaging using hyperpolarized magnetic resonance imaging. 相似文献
117.
Ferré N Camps J Prats E Girona J Gómez F Heras M Simó JM Ribalta J Joven J 《Metabolism: clinical and experimental》2002,51(5):609-615
Vitamin E is a lipid-soluble vitamin and an important antioxidant that protects lipoproteins and cell membranes from lipid peroxidation. The aims of the present study were to investigate, in patients with parenchymal liver cirrhosis, the following: (1) nutritional and vitamin E status in relation to compositional changes in lipoproteins; and (2) the effects of these alterations on the patients' plasma susceptibility to copper-mediated oxidation. Patients (n = 55) with liver cirrhosis and 25 healthy volunteers had vitamin E in serum and in isolated lipoprotein fractions analyzed by high-performance liquid chromatography (HPLC). Plasma susceptibility to peroxidation was measured by incubation with Cu(2+). Nutritional status was assessed by anthropometry. Vitamin E concentration was significantly decreased (P <.001) in the serum and in very-low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) in cirrhotic patients. The decrease was related to the degree of liver impairment. There were significant correlations between cholesterol and vitamin E concentrations in serum and in all the lipoprotein fractions (r between 0.72 and 0.89; P <.001) in cirrhotic patients, but there were no significant relationships between vitamin E and any of the anthropometric indices of nutritional status. The plasma maximal oxidation rate was significantly increased in cirrhotic patients (P <.01) and was inversely related to the serum concentration of vitamin E (P <.05). We conclude that lipoprotein alterations and not nutritional factors should be regarded as major factors explaining serum vitamin E reduction in patients with parenchymal liver cirrhosis, and that vitamin E depletion is associated with an increased plasma susceptibility to oxidation. 相似文献
118.
Struys EA Verhoeven NM Ten Brink HJ Wickenhagen WV Gibson KM Jakobs C 《Journal of inherited metabolic disease》2005,28(6):921-930
Summary We investigated the presence of hydroxyacid–oxoacid transhydrogenase (HOT), which catalyses the cofactor-independent conversion
of γ-hydroxybutyrate (GHB) to succinic semialdehyde coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG), in human liver extracts employing [2H6]GHB and 2-KG as substrates. We measured incorporation of 2H in D-[2H]2-HG using GC-MS analyses, providing evidence for HOT activity in humans. Kinetic characterization of HOT was undertaken
in forward and reverse directions. We employed [2H6]GHB and [2H4]2-KG as cosubstrates in order to develop a HOT activity assay in cultured human fibroblasts derived from patients with D-2-hydroxyglutaric aciduria. HOT activity was quantified in this system by the measurement of D-[2H5]2-HG production. Fibroblasts derived from patients with D-2-hydroxyglutaric aciduria showed normal HOT activities. Our results provide the first demonstration and preliminary kinetic
characterization of HOT activity in human tissues. 相似文献
119.
Karl F?rden May Brit Lund Trond Mogens Aal?kken Wijnand Eduard Per S?strand Sverre Lang?rd Johny Kongerud 《International journal of occupational and environmental health》2014,20(2):167-173
Background:
The long-term prognosis of repeated acute episodes of hypersensitivity pneumonitis (HP) is not well described. We report on a 10-year follow-up of a 10-person cluster from a Norwegian sawmill who had all experienced relapsing episodes of HP.Objectives:
To evaluate the health symptoms, work-related sick-leave, and lung function of 10 workers exposed to mold in a Norwegian sawmill.Methods:
Participants were evaluated at baseline and 10 years later at follow-up. A structured interview, measurement of serum IgG antibodies to Rhizopus microsporus (R. microsporus) antigens, lung function tests, high resolution computed tomography (HRCT) of the chest, and personal measurements of exposure to mold spores and dust were completed for each participant.Results:
At baseline, nearly all workers reported acute episodes of HP more than twice a month. At follow-up, both the frequency and intensity of symptoms had declined. Sick-leave was reduced and gas diffusing capacity improved – paralleling the gradually reduced air levels of mold spores.Conclusions:
In spite of an initially high occurrence of symptoms, long-term clinical and physiological outcome was good. With reduced exposure to mold spores, symptoms declined and lung function was restored. 相似文献120.
Imaging flow cytometry for automated detection of hypoxia‐induced erythrocyte shape change in sickle cell disease
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Eduard J. van Beers Leigh Samsel Laurel Mendelsohn Rehan Saiyed Kleber Y. Fertrin Christine A. Brantner Mathew P. Daniels James Nichols J. Philip McCoy Gregory J. Kato 《American journal of hematology》2014,89(6):598-603
In preclinical and early phase pharmacologic trials in sickle cell disease, the percentage of sickled erythrocytes after deoxygenation, an ex vivo functional sickling assay, has been used as a measure of a patient's disease outcome. We developed a new sickle imaging flow cytometry assay (SIFCA) and investigated its application. To perform the SIFCA, peripheral blood was diluted, deoxygenated (2% oxygen) for 2 hr, fixed, and analyzed using imaging flow cytometry. We developed a software algorithm that correctly classified investigator tagged “sickled” and “normal” erythrocyte morphology with a sensitivity of 100% and a specificity of 99.1%. The percentage of sickled cells as measured by SIFCA correlated strongly with the percentage of sickle cell anemia blood in experimentally admixed samples (R = 0.98, P ≤ 0.001), negatively with fetal hemoglobin (HbF) levels (R = ?0.558, P = 0.027), negatively with pH (R = ?0.688, P = 0.026), negatively with pretreatment with the antisickling agent, Aes‐103 (5‐hydroxymethyl‐2‐furfural) (R = ?0.766, P = 0.002), and positively with the presence of long intracellular fibers as visualized by transmission electron microscopy (R = 0.799, P = 0.002). This study shows proof of principle that the automated, operator‐independent SIFCA is associated with predictable physiologic and clinical parameters and is altered by the putative antisickling agent, Aes‐103. SIFCA is a new method that may be useful in sickle cell drug development. Am. J. Hematol. 89:598–603, 2014. © 2014 Wiley Periodicals, Inc. 相似文献