Loss of cables, a novel gene on chromosome 18q, in ovarian cancer.

Cables, a cyclin-dependent kinase (cdk) interacting protein, has recently been identified and mapped to human chromosome 18q11. Cables appears to be primarily involved in cell cycle regulation and cell proliferation. Overexpression of Cables in Hela and other cell lines inhibits cell proliferation and tumor formation. We hypothesize that loss of Cables expression is associated with ovarian cancer. To test our hypothesis, we examined Cables expression in the four most common subtypes of ovarian carcinomas: serous, endometrioid, mucinous, and clear cell. In addition, mucinous and serous borderline tumors were also included. Loss of Cables expression was observed at high frequency in ovarian serous (11 of 14 cases, 79%) and endometrioid (5 of 10 cases, 50%) carcinomas. In contrast, strong Cables staining was detected in all clear cell carcinomas (10 cases) and mucinous tumors (5 carcinomas and 5 borderline tumors). The majority of serous borderline tumors (11 of 14 cases, 79%) showed positive Cables staining, with the rest showing focal loss of Cables expression. Furthermore, RT-PCR revealed the lack of Cables mRNA in a human ovarian cancer xenograft. No correlation was noted between loss of Cables and histologic grade, tumor stage, and survival. In conclusion, our results indicate that loss of Cables is common in ovarian serous and endometrioid carcinomas and imply that Cables may be involved in the pathogenesis of these two types of ovarian carcinomas.     

非体外循环冠状动脉搭桥术早期疗效的探讨

目的：探讨非体外循环心脏不停跳冠状动脉搭桥术的安全性和早期临床效果。方法：自2000年7月至2002年3月51例冠心病患接受冠状动脉搭桥手术，按手术方式分为非体外循环组（n=21）和传统体外循环组（n=30)。将两组病人的术前临床资料，手术方式，术后并发症和疗效等进行对比分析。结果：两组术前临床资料无明显差异。非体外循环组手术时间，术后出血输血量，呼吸机辅助时间显少于体外循环组，并且术后心律失常的发生率明显降低（P均＜0．05），其他重要器官的并发症发生率亦低于体外循环组。两组平均随访9．6个月，心绞痛均消失，心功能改善，活动量明显增加。结论：非体外循环冠状动脉搭桥术是安全可行的，并发症少，近期疗效满意。     

2型糖尿病患者炎性细胞因子检测的意义

目的　探讨炎性细胞因子IL 6、IL 8及TNF α在 2型糖尿病发病机制中的意义。方法　用双抗体夹心ELISA法检测 3 2例糖尿病患者及 2 8名正常人血清IL 6、IL 8及TNF α浓度并进行比较。结果　 2型糖尿病患者血清IL 6、IL 8及TNF α水平均明显高于正常人 (p <0 0 1)。结论　患者体内高于水平的IL 6、IL 8及TNF α可能参与糖尿病的发生发展 ,3者相互作用 ,促进 2型糖尿病进展。     

黄芪天麻对老年心脑血管疾病患者发中微量元素影响的研究

黄芪、天麻中含有较丰富地人体必需微量元素。而人体内的微量元素含量的多少与生长、发育、健康、衰老及心脑血管疾病的发生和防治等均有密切关系。我们检查了３９例健康老年人及９８例患有心脑血管疾病老年人头发中的６种微量元素，上述病人在服用黄芪、天麻前后头发中微量元素的变化。研究结果表明黄芪、天麻对调节体内微量元素的含量，预防和治疗心脑血管疾病有较好地效果。     

手术对晚期卵巢癌患者机体免疫功能的影响

本文对12例晚期卵巢癌患者术前、术后的免疫功能分别进行了研究,以15例健康女性为对照。结果表明,卵巢癌患者的天然杀伤细胞活性及 T辅助细胞减低,T 抑制细胞增多,T 辅助细胞与 T 抑制细胞比值下降,与对照组相比,差别具有显著性。经瘤细胞减灭术后,机体免疫功能有不同程度的恢复,但总 T 淋巴细胞改变不明显。     

反映手术间利用效率的统计指标与方法探讨

目的 探索反映手术室工作状态的指标和方法，为了解科室需求、合理利用手术室资源奠定基础。方法 提取医院局域网中有关手术安排、手术过程、麻醉等信息，通过常用统计指标、统计图表，反映手术室、手术科室、手术间的工作状态。结果 工作日手术问日均工作6．34小时，节假日日均工作0．6l小时。工作日13点后有近l／3的空台，14点后近l／2空台。个别手术间利用率偏低。结论 1．由在用手术间时点统计结果可见，14点后接台手术尚有一定潜力，节假日安排手术潜力更大；2．工作日间均工作时数已近满负荷，若要进一步提高手术间的利用，需加强麻醉科和手术室力量。     

心电图短P-R间期观测研究

目的：探讨和鉴别心电图短Ｐ－Ｒ间期的产生原因，为临床诊断、治疗提供依据。方法：检测１１５例受检者的常规导联心电图（ＲＬＥＣＧ）、头胸导联心电图（ＨＣＥＣＧ）、食管导联心电图（ＥＬＥＣＧ），对其中５５例临床可疑心律失常和ＲＬＥＣＧ示短Ｐ－Ｒ间期者行食管心房调搏电生理检查。结果：①Ｐ波出现率和振幅均值比较ＥＬＥＣＧ＞ＨＣＥＣＧ＞ＲＬＥＣＧ（Ｐ＜０．０１），出现率分别为１００％、９５％、９１％。②ＨＣＥＣＧＰ－Ｒ段均值（Ｐ＜０．０１）及变异系数＜ＲＬＥＣＧ。③ＲＬＥＣＧ波幅低、图形多变是产生心电图短Ｐ－Ｒ间期的主要原因。结论：作为无创伤性检查方法，ＨＣＥＣＧ和ＥＬＥＣＧ可以对短Ｐ－Ｒ间期者进行初步筛选，而经食管心房调搏能检测ＥＣＧ短Ｐ－Ｒ间期的电生理特性，对鉴别诊断有肯定价值。     

Partial mastectomy with axillary lymph node dissection and radiotherapy as a new treatment modality of breast cancer (I).

This is the first preliminary report among two consecutive papers. Partial mastectomy(PM), axillary lymph node dissection(AD) and radiotherapy (RT) were performed on seventeen operable breast cancer patients who had been admitted from April 1991 to March 1992 to the department of surgery, Yongdong Severance Hospital for improved cosmetic appearance and better survival rate. Of seventeen patients, 47% were T1 lesion and 76% were stage I and II. Extensive intraductal component(EIC) within or around the tumor was also analyzed. Twenty nine per cent of the patients were EIC positive. The mean number of axillary lymph nodes was 21.5 after PM with AD and 20.5 after mastectomy. For radiotherapy, 4,500 rad was delivered to the breast parenchyma and 1,600 rad of boost to the primary tumor site using the electron beam method after surgery. All patients have since been living well without any local recurrence and were satisfied with breast preservation for the one-year follow-up period. We concluded that the PM, AD and RT can be another surgical treatment modality of breast cancer. A longer follow-up data will be followed on the second paper.