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71.
Abou-Dakn Michael Alexy Ute Beyer Kirsten Cremer Monika Ensenauer Regina Flothkötter Maria Geene Raimund Hellmers Claudia Joisten Christine Koletzko Berthold Mata Jutta Schiffner Ulrich Somm Irene Speck Melanie Weißenborn Anke Wöckel Achim 《Monatsschrift für Kinderheilkunde》2022,170(10):925-928
Monatsschrift Kinderheilkunde - 相似文献
72.
Multicentre study for diagnostic evaluation of an assay for simultaneous detection of antibodies to HIV-1, HIV-2 and HIV-1 subtype 0 (HIV-0) 总被引:1,自引:0,他引:1
P. Bachmann Dr. J. Beyer Dr. S. Brust Dr. rer. nat. W. Engelhardt Prof. Dr. L. G. Gürtler Prof. Dr. K. O. Habermehl Prof. Dr. M. Stöffler-Meilicke Dr. A. Karakassopoulos Dr. U. Michl Dr. A. Mühlbacher Dr. R. Thorstensson 《Infection》1995,23(5):322-333
Summary The aim of the study was to evaluate a new ELISA for detection of HIV-1, HIV-2 and HIV-1 subtype 0 (HIV-0) antibodies. The assay format is based on the antigen sandwich principle. To enable specific detection of HIV-0 antibodies, in addition to HIV-1 and HIV-2 antigens HIV-0 antigen is used for coating the solid phase and for the conjugate. The results show that all 12 HIV-0 samples tested were detected with a high degree of reactivity, as were all the 1,144 anti-HIV-1 and 424 anti-HIV-2 positive samples. The capacity of the test to enable early detection of seroconversions is equivalent to that of other sandwich ELISAs. The specificity of the assay was determined to be 99.89/99.94% (initial/after retest) using 58,366 samples, which is superior to the other ELISAs used for comparison. Even with difficult samples (i.e. samples of African origin, samples known to cause false-positive reactivity in different ELISAs, or samples containing potential interference factors) there were very few false-positive reactions. Therefore, the new assay is well suited for screening blood donations as well as for evaluating samples from patients of different geographic origin.
Multizenterstudie zur Bewertung eines Testsystems für den gleichzeitigen Nachweis von Antikörpern gegen HIV-1, HIV-2 und HIV-1 Subtyp 0 (HIV-0)
Zusammenfassung Ziel der Studie war die Bewertung eines neuen ELISA zum Nachweis von Antikörpern gegen HIV-1, HIV-2 und dem HIV-1 Subtyp 0 (HIV-0). Der Test beruht auf dem Antigen-Sandwich-Prinzip. Für den spezifischen Nachweis von Antikörpern gegen HIV-0 wird HIV-0-Antigen zusammen mit HIV-1 und HIV-2-Antigen zur Beschichtung der festen Phase und für das Konjugat verwandt. Die Ergebnisse zeigten, daß alle 12 getesteten HIV-0-Proben mit hoher Reaktivität nachweisbar waren sowie alle 1 144 anti-HIV-1- und 424 anti-HIV-2-positiven Proben. Die Fähigkeit des Testsystems zum frühen Nachweis einer Serokonversion entspricht derjenigen anderer Sandwich-ELISAs. Die Spezifität des Tests wurde an 58 366 Proben mit 99,89/99,94% (initial/Wiederholungstestung) ermittelt und liegt über derjenigen von anderen zum Vergleich herangezogenen ELISAs. Auch bei schwierigen Proben (zum Beispiel Proben aus Afrika, Proben, bei denen falsch-positive Reaktivität in verschiedenen ELISAs beobachtet worden war oder Proben, die mögliche Interferenzfaktoren enthalten) fanden sich nur sehr wenige falsch positive Reaktionen. Der neue Test eignet sich daher gut für das Screening von Blutspenden und für die Beurteilung von Proben von Patienten unterschiedlicher geographischer Herkunft.相似文献
73.
The renal circulation, which treats 25% of the cardiac output, is organized and regulated in unique patterns. Gap junction channels may contribute to the control of vascular tone by transmitting intracellular signals rapidly between cells of the blood vessel. We investigated the distribution patterns of the major vascular gap junction proteins in the murine kidney by immunofluorescence staining of frozen sections, and connexin40 (Cx40) was the most prominent connexin detected. The endothelial cells of large vessels within the kidney consistently showed abundant Cx40 immunoreactivity, but small vessels showed unique distributions of Cx40 along their courses within the kidney. Cx40 immunoreactivity between endothelial cells was abundant in the interlobular arteries and the proximal portion of the afferent arterioles, but was significantly decreased when arterioles approached the glomerulus. No Cx40 immunoreactivity was detected in the region of the glomerular isthmus where the afferent and efferent arterioles join the glomeruli, although glomeruli showed very intense patchy staining for Cx40. Intense patchy staining for Cx40 was also found in the modified smooth muscle cells of the juxtaglomerular apparatus, but none was detected in smooth muscle cells elsewhere in the vasculature. Taken together, these data suggest that the abundance of Cx40-containing gap junctions may be important for coordinating function of cells within individual blood vessels, while their absence in juxtaglomerular regions of the arterioles may prevent conduction of signals between the glomerulus and afferent or efferent arterioles. 相似文献
74.
Carla Palleis MD Julia Sauerbeck Leonie Beyer MD Stefanie Harris Julia Schmitt Estrella Morenas-Rodriguez PhD Anika Finze Alexander Nitschmann Francois Ruch-Rubinstein Florian Eckenweber Gloria Biechele Tanja Blume MSc Yuan Shi PhD Endy Weidinger MD Catharina Prix MD Kai Bötzel MD Adrian Danek MD Boris-Stephan Rauchmann MD Sophia Stöcklein MD Simon Lindner PhD Marcus Unterrainer MD Nathalie L. Albert MD Christian Wetzel PhD Rainer Rupprecht MD Axel Rominger MD Peter Bartenstein MD Jochen Herms MD Robert Perneczky MD Christian Haass PhD Johannes Levin MD Günter U. Höglinger MD Matthias Brendel MD 《Movement disorders》2021,36(4):883-894
75.
Carla Palleis MD Matthias Brendel MD Anika Finze Endy Weidinger MD Kai Bötzel MD Adrian Danek MD Leonie Beyer MD Alexander Nitschmann Maike Kern Gloria Biechele Boris-Stephan Rauchmann MD Jan Häckert MD Matthias Höllerhage MD Andrew W. Stephens MD PhD Alexander Drzezga MD Thilo van Eimeren MD Victor L. Villemagne MD Andreas Schildan PhD Henryk Barthel MD Marianne Patt PhD Osama Sabri MD German Imaging Initiative for Tauopathies Peter Bartenstein MD Robert Perneczky MD Christian Haass PhD Johannes Levin MD Günter U. Höglinger MD 《Movement disorders》2021,36(9):2104-2115
76.
Celina Beyer Christian Schwahn Georg Meyer Andreas Söhnel 《The Journal of prosthetic dentistry》2021,125(5):805-814
Statement of problemWhich restorative material is best suited for treating the posterior molar region is unclear. As dentists presumably choose the most appropriate restoration, German dentists were asked how their own molars were treated.PurposeThe purpose of this survey study was to examine molar restorations and their durability in German dentists.Material and methodsIn the official journal of the Federal Dental Association, German dentists were asked to participate in an online survey that gathered demographic data and information on the type and durability of their molar restorations. To reduce selection bias, the data were weighted for region, sex, and age of the dentist.ResultsThe data set consisted of 1719 molars from 288 dentists. Restorations included gold inlays (25%), composite resin (24.3%), amalgam (11.8%), ceramic inlays (5.4%), glass ionomer cement or compomer (0.8%), gold crowns (21.8%), ceramic crowns (6.6%), and metal-ceramic crowns (4.3%). Notable differences were identified based on the sex, age, and region of the dentist. Women selected increased esthetic options, as did young dentists. Restorations made of gold, amalgam, and base metal had the highest longevity, at more than 20 yearsConclusionsAmong German dentists, restorations with metallic materials dominated in molars, with gold accounting for the largest share. In recent years, the proportion of tooth-colored restorations has increased, and in particular, young dentists use composite resin and ceramics. 相似文献
77.
78.
Marco Dollinger Lukas Philipp Beyer Michael Haimerl Christoph Niessen Ernst-Michael Jung Florian Zeman Christian Stroszczynski Philipp Wiggermann 《Diagnostic and interventional radiology (Ankara, Turkey)》2015,21(6):471-475
PURPOSE
We aimed to describe the frequency of adverse events after computed tomography (CT) fluoroscopy-guided irreversible electroporation (IRE) of malignant hepatic tumors and their risk factors.METHODS
We retrospectively analyzed 85 IRE ablation procedures of 114 malignant liver tumors (52 primary and 62 secondary) not suitable for resection or thermal ablation in 56 patients (42 men and 14 women; median age, 61 years) with regard to mortality and treatment-related complications. Complications were evaluated according to the standardized grading system of the Society of Interventional Radiology. Factors influencing the occurrence of major and minor complications were investigated.RESULTS
No IRE-related death occurred. Major complications occurred in 7.1% of IRE procedures (6/85), while minor complications occurred in 18.8% (16/85). The most frequent major complication was postablative abscess (4.7%, 4/85) which affected patients with bilioenteric anastomosis significantly more often than patients without this condition (43% vs. 1.3%, P = 0.010). Bilioenteric anastomosis was additionally identified as a risk factor for major complications in general (P = 0.002). Minor complications mainly consisted of hemorrhage and portal vein branch thrombosis.CONCLUSION
The current study suggests that CT fluoroscopy-guided IRE ablation of malignant liver tumors may be a relatively low-risk procedure. However, patients with bilioenteric anastomosis seem to have an increased risk of postablative abscess formation.About 70% of hepatic metastases are nonresectable because of their anatomic location, the presence of comorbidities, or limited hepatic functional reserve (1). In these patients and in case of nonresectable primary liver tumors, percutaneous thermal ablation procedures, such as radiofrequency (RF) and microwave ablation, have become effective tools for treating hepatic malignancies (2–4). However, the effectiveness of RF and microwave treatment may be limited, either because of thermal damage to temperature-sensitive structures located in close proximity to the target tissue (5) or because of incomplete ablation of tumors adjacent to major hepatic vessels due to a phenomenon commonly termed “heat-sink effect” (6–10) which describes the loss of the applied thermal energy through the blood flow in those major vessels, whereby the effective energy application remains inadequate to ablate the target lesion.Irreversible electroporation (IRE) is a theoretically nonthermal ablation technique that delivers a series of high-voltage millisecond electrical pulses to the surrounding tissue, thus leading to irreversible disruption of the integrity of cell membranes and subsequent cell death by apoptosis (11–14). IRE may overcome the problems raised with thermal ablation: previous animal studies reported that bile ducts, blood vessels, nerves, and connective tissues are affected by IRE; however, regeneration is possible to some extent due to preservation of the tissue architecture (12, 13, 15–19). Moreover the feasibility of inducing cell death up to a vessel wall without any perivascular sparing was shown with IRE (12, 13, 18). The safety of IRE in the treatment of humans has been described (20). First reports have described potential complications after IRE, such as hemorrhage requiring blood transfusion (1.2%, two of 167 ablation procedures), portal vein thrombosis (3.2%, one of 31 ablation procedures), injury to bile ducts (1.8%, three of 167 ablation procedures), and infection (3.6%, six of 167 ablation procedures) (21, 22). However, few data are available for evaluating the potential risk factors associated with the occurrence of post-IRE complications.The purpose of this study was to review the frequency of mortality and morbidity after computed tomography (CT) fluoroscopy-guided liver IRE conducted at a single center and assess the factors influencing the occurrence of major complications. 相似文献79.