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991.
Srikar Anagoni Nagaraja Mudhigeti Mohan Alladi Verma Anju Padmalatha AM Usha Kalawat 《Indian journal of medical microbiology》2022,40(3):427-432
PurposeA large number of new molecular or virology laboratories have been established to increase the testing capacity for SARS-CoV-2. Due to heavy workload, there is delay in testing of samples. In order to avoid the negative effect of delayed testing on RTPCR results guidelines are issued from WHO and CDC to transport samples in cold chain. However, in pandemic situations the recommended guidelines for transport and storage conditions are often compromised. This study was conducted to evaluate the effect of sample storage conditions at different temperatures on the results of RT PCR test.MethodsTotal 275 samples were included in this study, among these 126 samples tested positive and 149 samples tested negative. All samples were aliquoted into two and the aliquotes stored in duplicate at 4 ?°C and room temperature. All aliquots stored at both the temperatures were tested by RTPCR every 24 hours up to 5 days.ResultsDiagnostic accuracy decreased from day1 to day 5 at both the storage temperatures i,e 4 ?°C and room temperature in comparison to the initial day results. Positivity decreased on an average of 9.02% at 4 ?°C and at 9.27% at room temperature per day. Among total 126 positive samples on an average false negative and failure of internal control at 4 ?°C and room temperature was 8.86%, 8.22% and 3.64%, and 4.12%, respectively. All the samples with CT value ?< ?30 remained positive at both temperatures up to 5 days. Few samples with >30 CT value showed variable results i.e. positive, negative or internal control failure from day 1 (2nd day after sample collection) onwards.ConclusionsThere was no significant difference between RT PCR results of samples stored at 4 ?°C and room temperature up to 5 days of collection. However internal control failure was more in samples stored at room temperature. Therefore, samples received without cold chain also may be processed by RTPCR and should not be rejected. 相似文献
992.
Evaluation of the performance of hysterosalpingo contrast sonography in 500 consecutive, unselected, infertile women 总被引:1,自引:1,他引:1
Hamilton JA; Larson AJ; Lower AM; Hasnain S; Grudzinskas JG 《Human reproduction (Oxford, England)》1998,13(6):1519-1526
The performance of hysterosalpingo contrast sonography (Hy Co Sy) as a
first-line, outpatient investigation of tubal patency was examined in 500
consecutive, infertile women, at one centre. Hy Co Sy was completed in 463
(92.6%) cases, using a galactose microbubble contrast agent (Echovist-200)
and transvaginal sonography. Initial plain scanning identified adnexal
pathology in 198 women (39.6%). Examination with Echovist was attempted for
905 tubes and only 67 (7.4%) were not assessable; after the first 100 women
this decreased to 35 tubes (4.8%). A sonographic appearance compatible with
blocked tubes was found on 118 (14.1%) occasions but it was also possible
to identify variations in the appearance/filling/spilling patterns of
individual tubes which increased the number assessed as abnormal to 193
(23.0%). Comparison with laparoscopy and dye chromopertubation findings
from the past three years was possible for 185 (37%) women, representing
282 tubes, which gave Hy Co Sy an overall concordance rate of 85.8%,
sensitivity of 90.4%, specificity of 70.3%, positive predictive value of
91.2% and negative predictive value of 68.2%. Some 51.0% of women described
only mild discomfort and there were no significant postprocedure
complications. Hy Co Sy appears to be an acceptable first- line screen and
may select out women in whom more invasive investigations are likely to
reveal pathology.
相似文献
993.
994.
Linkage analysis of candidate regions for coeliac disease genes 总被引:5,自引:0,他引:5
Houlston RS; Tomlinson IP; Ford D; Seal S; Marossy AM; Ferguson A; Holmes GK; Hosie KB; Howdle PD; Jewell DP; Godkin A; Kerr GD; Kumar P; Logan RF; Love AH; Johnston S; Marsh MN; Mitton S; O'Donoghue D; Roberts A; Walker-Smith JA; Stratton MF 《Human molecular genetics》1997,6(8):1335-1339
A strong HLA association is seen in coeliac disease [specifically to the
DQ(alpha1*0501,beta1*0201 heterodimer], but this cannot entirely account
for the increased risk seen in relatives of affected cases. One or more
genes at HLA-unlinked loci also predispose to coeliac disease and are
probably stronger determinants of disease susceptibility than HLA. A recent
study has proposed a number of candidate regions on chromosomes 6p23
(distinct from HLA), 6p12, 3q27, 5q33.3, 7q31.3, 11p11, 15q26, 19p13.3,
19q13.1, 19q13.4 and 22cen for the location of a non-HLA linked
susceptibility gene. We have examined these regions in 28 coeliac disease
families by linkage analysis. There was excess sharing of chromosome 6p
markers, but no support for a predisposition locus telomeric to HLA. No
significant evidence in favour of linkage to coeliac disease was obtained
for chromosomes 3q27, 5q33.3, 7q31.3, 11p11, 19p13.3, 19q13.1, 19q13.4 or
22cen. There was, however, excess sharing close to D15S642. The maximum
non-parametric linkage score was 1.99 (P = 0.03). Although the evidence for
linkage of coeliac disease to chromosome 15q26 is not strong, the well
established association between coeliac disease and insulin dependent
diabetes mellitus, together with the mapping of an IDDM susceptibility
locus (IDDM3) to chromosome 15q26, provide indirect support for this as a
candidate locus conferring susceptibility to coeliac disease in some
families.
相似文献
995.
Germline mutations of the MEN1 gene in familial multiple endocrine neoplasia type 1 and related states 总被引:9,自引:0,他引:9
Agarwal SK; Kester MB; Debelenko LV; Heppner C; Emmert-Buck MR; Skarulis MC; Doppman JL; Kim YS; Lubensky IA; Zhuang Z; Green JS; Guru SC; Manickam P; Olufemi SE; Liotta LA; Chandrasekharappa SC; Collins FS; Spiegel AM; Burns AL; Marx SJ 《Human molecular genetics》1997,6(7):1169-1175
Familial multiple endocrine neoplasia type 1 (FMEN1) is an autosomal
dominant trait characterized by tumors of the parathyroids, gastro-
intestinal endocrine tissue, anterior pituitary and other tissues. We
recently cloned the MEN1 gene and confirmed its identity by finding
mutations in FMEN1. We have now extended our mutation analysis to 34 more
unrelated FMEN1 probands and to two related states, sporadic MEN1 and
familial hyperparathyroidism. There was a high prevalence of heterozygous
germline MEN1 mutations in sporadic MEN1 (8/11 cases) and in FMEN1 (47/50
probands). One case of sporadic MEN1 was proven to be a new MEN1 mutation.
Eight different mutations were observed more than once in FMEN1. Forty
different mutations (32 FMEN1 and eight sporadic MEN1) were distributed
across the MEN1 gene. Most predicted loss of function of the encoded menin
protein, supporting the prediction that MEN1 is a tumor suppressor gene. No
MEN1 germline mutation was found in five probands with familial
hyperparathyroidism, suggesting that familial hyperparathyroidism often is
caused by mutation in another gene or gene(s).
相似文献
996.
997.
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1000.
Extracorporeal shockwave therapy for the treatment of chronic wound of lower extremity: current perspective and systematic review
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Mohammed TA Omar Rehab FM Gwada Afaf AM Shaheen Raoul Saggini 《International wound journal》2017,14(6):898-908
The purpose of this study was to provide an up‐to‐date review for the accurate estimation of the efficacy of extracorporeal shock wave therapy (ESWT) on the healing of chronic wounds on the lower extremity (CWLE). A systematic review of 10 databases for clinical trials about ESWT in the management of CWLE published between 2000 and 2016 was performed. A total of 11 studies with 925 patients were found. Expert therapists assessed the methodological qualities of the selected studies using the Physiotherapy Evidence Database (PEDro) scale and categorised each study according to Sackett's levels of evidence. Eight studies were categorised as level II; two studies were categorised as level III and one study was categorised as level V. In conclusion, this review demonstrated mild to moderate evidence to support the use of ESWT as an adjuvant therapy with a standardised wound care programme. However, it is difficult to draw firm conclusions about the efficacy of ESWT. So, future researches with high methodological quality are required to assess the efficacy and cost‐effectiveness of this relatively new physical therapy application. 相似文献