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排序方式: 共有427条查询结果,搜索用时 15 毫秒
121.
PL Mitchell VB Shepherd HM Proctor M Dainton SD Cabral CR Pinkerton 《Archives of disease in childhood》1994,70(3):237-240
Peripheral blood stem cells (PBSC) were used to augment autologous bone marrow transplantation (ABMT), aiming to hasten engraftment after high dose treatment in a group of heavily pretreated patients. PBSC were obtained by leukapheresis during the rebound after standard chemotherapy. In 11 patients aged 7-17 years, high dose chemotherapy consisted of busulphan 16 mg/kg orally with melphalan 160 mg/m2 intravenously for seven patients, and melphalan 200 mg/m2 intravenously alone for four. The median number of granulocyte-macrophage colony forming units in the reinfused PBSC was 3.42 x 10(4)/kg (3.03-18.01) and bone marrow 12.4 x 10(4)/kg (4.16-28.6). Neutrophil recovery to > or = 0.5 x 10(9)/l and platelet transfusion independence occurred at a median of 14 days (11-18) and 22 days (9-84) respectively. In five patients the early engraftment was transient with neutrophils again dropping below 0.5 x 10(9)/l then slowly recovering. There was one toxic death due to sepsis. PBSC harvesting in these children was undertaken without interrupting routine chemotherapy and without the use of bone marrow growth factors. In some patients PBSC failed to influence engraftment and the use of combined chemotherapy and growth factor priming for PBSC collection may give improved results. 相似文献
122.
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124.
Embryo transfer--can we learn anything new from the observation of junctional zone contractions? 总被引:2,自引:6,他引:2
Lesny P; Killick SR; Tetlow RL; Robinson J; Maguiness SD 《Human reproduction (Oxford, England)》1998,13(6):1540-1546
To assess whether embryo transfer can alter junctional zone contractility,
we studied the effect of easy and difficult mock transfers in 14 oocyte
donors during in-vitro fertilization (IVF) cycles. An Echovist bolus (30
microl) was used to represent embryos and transfer medium. An 'easy'
transfer was judged to be an atraumatic insertion of the catheter without
touching the uterine fundus. A 'difficult' embryo transfer was mimicked by
deliberately touching the uterine fundus twice with the soft end of the
cannula. Transvaginal scan images were recorded, digitized and converted
into five times normal speed to allow us to evaluate junctional zone
contractility. Easy mock embryo transfers did not change endometrial
mechanical activity. Echovist remained in the upper part of the uterine
cavity and was not dispersed after 45 min. A difficult procedure generated
strong random waves in the fundal area and waves from fundus to cervix
which relocated the Echovist in six out of seven cases. We observed
movements of the transfer bolus from the upper part of the uterus towards
the cervix (four cases) and into Fallopian tubes (two patients). Our study
confirms that the mechanical activity of the uterus is capable of
relocating intrauterine embryos and that this activity depends on physical
stimulation. Junctional zone contractions can be implicated in cases of
IVF/embryo transfer failure or ectopic gestation.
相似文献
125.
Great progress has been achieved during the last 20 years inthe field of ovulation induction in patients with polycysticovary syndrome (PCOS). Clomiphene citrate remains the firstline of treatment for all anovulatory women with PCOS, sincein properly selected patients the cumulative pregnancy rateapproaches that in normal women. Human urinary gonadotrophinshave been used extensively for ovulation induction but the developmentof low-dose regimens has opened a new era in the managementof anovulation related to PCOS. This article discusses the mainadvantages and disadvantages of the principal methods and regimenscurrently used for ovulation induction in patients with PCOSincluding clomiphene citrate, gonadotrophins, pulsatile gonadotrophin-releasinghormone (GnRH) and GnRH agonists. It also discusses new drugsdiscovered recently, particularly recombinant gonadotrophinsand GnRH antagonists, and provides some thoughts regarding theiruse in future protocols. Finally, based on the discovery ofnew ovarian substances which specifically control luteinizinghormone (LH) secretion, this article develops assumptions onpossible implications of these substances in the pathophysiologyof PCOS and their potential use in the management of the syndrome. 相似文献
126.
Somatic mutations are frequent and increase with age in human kidney epithelial cells 总被引:6,自引:0,他引:6
Martin GM; Ogburn CE; Colgin LM; Gown AM; Edland SD; Monnat RJ Jr 《Human molecular genetics》1996,5(2):215-221
We have used a primary cloning assay to determine the frequency of 6-
thioguanine (TG)-resistant tubular epithelial cells in kidney tissue from
72 human donors ranging in age from 2 to 94 years. The frequency of
TG-resistant mutants ranged from approximately 5 x 10(-5) for donors in the
first decade of life to approximately 2.5 x 10(-4) for donors in the eighth
and later decades of life. Two different statistical analyses indicated
that this increase in mutant frequency is exponential with age. We also
observed a 2-fold higher TG-resistant mutant frequency in nephrectomy
kidneys containing a coincident renal carcinoma. DNA sequence analyses
revealed HPRT gene mutations in each of 14 TG-resistant mutants from seven
unrelated donors. Thirteen of these 14 mutants resulted from independent
mutational events. These results suggest that somatic mutations are common
in renal--and perhaps in other human--epithelia, and thus could play an
important role in the genesis of age-associated disease.
相似文献
127.
The predictive value of the zona-free hamster egg penetration test in relation to in-vitro fertilization at various insemination concentrations 总被引:2,自引:0,他引:2
Zainul Rashid MR; Fishel SB; Thornton S; Hall JA; Ndukwe G; Aloum M; Fleming SD 《Human reproduction (Oxford, England)》1998,13(3):624-629
The aim of the study was to evaluate the predictive value of the zona- free
hamster egg penetration test (ZHEPT) for success in in-vitro fertilization
(IVF) at various insemination concentrations ranging between 0.1 and
>0.6 x 10(6)/ml. The ZHEPT was assessed using sperm samples from 87
couples undergoing IVF treatment. A similar test was simultaneously
performed on the same semen sample following ionophore induction of the
acrosome reaction (ZHEPTii test). Both the tests were poorly correlated
with the fertilization rate of IVF at all the insemination concentrations
except at >0.6 x 10(6)/ml, when there was good correlation between the
ZHEPTii test and the fertilization rate. Following exclusion of two cases
with an oocyte problem, further statistical analysis revealed that both the
ZHEPT and ZHEPTii tests were poorly correlated with fertilization rate in
IVF in this treatment group. This study suggests that the ZHEPT (with and
without ionophore induction of the acrosome reaction) has a poor predictive
value for the success of fertilization in IVF treatment at any insemination
concentration.
相似文献
128.
129.
M Tarchouli B Ait Idir N Bouhabba SD El Jaouhari T Salaheddine Y Hnach A Zentar 《Annals of the Royal College of Surgeons of England》2021,103(3):e77
Trichobezoar is a rare condition that occurs mostly in young women with psychiatric disorders such as trichotillomania and trichophagia. We report the case of a giant gastric trichobezoar in a 21-year-old woman who presented with chronic abdominal pain, vomiting and weight loss. Abdominal examination revealed a large epigastric mass. Endoscopic and imaging findings were highly suggestive of a gastric trichobezoar. Surgical extraction of the huge hair mass was successfully performed through an open gastrotomy. Postoperatively, history of a neglected chronic depression with suicidal ideation was diagnosed. Consequently, the patient was referred to the psychiatric department for mental healthcare, to prevent trichobezoar recurrences. 相似文献
130.
A Pro51Ser mutation in the COCH gene is associated with late onset autosomal dominant progressive sensorineural hearing loss with vestibular defects 总被引:14,自引:0,他引:14
de Kok YJ; Bom SJ; Brunt TM; Kemperman MH; van Beusekom E; van der Velde- Visser SD; Robertson NG; Morton CC; Huygen PL; Verhagen WI; Brunner HG; Cremers CW; Cremers FP 《Human molecular genetics》1999,8(2):361-366
We analysed a Dutch family with autosomal dominant non-syndromic
progressive sensorineural hearing loss and mapped the underlying gene
defect by genetic linkage analysis to a 11.0 cM region overlapping the
DFNA9 interval on chromosome 14q12-q13. Clinically, the Dutch family
differs from the original DFNA9 family by a later age at onset and a more
clearly established vestibular impairment. A gene that is highly and
specifically expressed in the human fetal cochlea and vestibule, COCH
(previously described as Coch5B2 ), was mapped to the DFNA9 critical
region. Sequence analysis revealed a 208C-->T mutation in the COCH gene,
resulting in a Pro51Ser substitution in the predicted protein in all
affected individuals of the family but not in unaffected family members and
200 control individuals. The same mutation was also identified in three
apparently unrelated families with a similar phenotype, suggesting the
presence of a Dutch founder mutation. The function of COCH is unknown but
several characteristics of the protein point to a structural role in the
extracellular matrix. The mutant serine at position 51 is situated between
cysteines and possibly interferes with proper COCH protein folding or its
interaction with extracellular matrix proteins.
相似文献