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71.
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New reference allelic ladders to improve allelic designation in a multiplex STR system 总被引:4,自引:0,他引:4
R. A. L. Griffiths M. D. Barber P. E. Johnson S. M. Gillbard M. D. Haywood C. D. Smith J. Arnold T. Burke A. J. Urquhart P. Gill 《International journal of legal medicine》1998,111(5):267-272
This paper reports the composition of a new reference allelic ladder mixture for use with a multiplex DNA profiling system
consisting of six short tandem repeat loci. The loci included in this mixture are HUMTH01, D21S11, D18S51, D8S1179, HUMVWAF31/A,
HUMFIBRA/FGA and an amelogenin sex test. Sequence analysis of individual ladder alleles was carried out and allelic designations
made in accordance with the recommendations of the International Society of Forensic Haemogenetics (1992; 1994). A series
of rare alleles which increase the range of alleles previously reported were identified. By including some of the rare alleles
into the ladder marker system, we have significantly improved the ability to identify new alleles in unknown samples.
Received: 12 August 1997 / Received in revised form: 7 November 1997 相似文献
74.
Victor L. Roggli Philip C. Pratt Arnold R. Brody 《American journal of industrial medicine》1993,23(4):605-614
Although the association between asbestos exposure and malignant mesothelioma is indisputable, controversy continues regarding the relative contribution of the various types of asbestos fibers to the development of mesothelioma. We examined the types of asbestos fibers recovered from lung parenchyma in more than 90 cases of malignant mesothelioma from the United States, using an analytical scanning electron microscope. Almost half of the patients were former asbestos insulators or shipyard workers. The fibers were recovered from lung tissues obtained at autopsy or surgical resection by means of a sodium hypochlorite digestion procedure. Amosite absestos was identified in 81% of the cases and accounted for 58% of all fibers 5 μm or greater in length. Tremolite/actinolite/anthophyllite were identified in 55% of the cases and accounted for 10% of all fiber types. Chrysotile was identified in 21% of the cases and accounted for 3% of fibers exceeding 5 μm in length. Crocidolite was found in 16% of the cases and accounted for 3% of fibers exceeding 5 μm in length. Nonasbestos mineral fibers (commonly found in the lungs of the general population) were observed in 71% of the cases and accounted for 25% of all fibers 5 μm or greater in length. The findings in this study are at odds with the assertion that crocidolite asbestos is responsible for most mesotheliomas in the United States. © 1993 Wiley-Liss, Inc. 相似文献
75.
76.
Genesis of papillar pit. Microscopy study in a newborn infant 总被引:1,自引:0,他引:1
The rare microscopic picture of a pit of the optic disk is described. The recess extended from the physiological cup of the optic disk to the former cleft of the optic stalk at the point of entry of the ophthalmic artery. There was no disk coloboma. This result is in good agreement with von Szily's paper on the genesis of crater-like holes in the optic disk as a residue of the cavity of the primordial epithelial papilla. In this case there was no communication of the vitreous cavity with a subretinal space, which is suspected of being the cause of the frequent concomitant serous detachment of the macula. 相似文献
77.
78.
Zusammenfassung
Im Jahr 1988 wurde erstmals von der m?glichen Wirksamkeit der Valproins?ure in der Migr?neprophylaxe berichtet. Seit 1992
sind insgesamt 359 Patienten in 5 Doppelblindstudien untersucht. In allen Studien ergab sich eine signifikant überlegene Wirksamkeit
im Vergleich zu Placebo, eine Studie belegt einen gleichen Effekt wie Propranolol. Es fehlen noch Wirksamkeitsanalysen, die
sich am Plasmaspiegel orientieren. Die Nebenwirkungen entsprechen denen aus der Epilepsietherapie, scheinen aber im Vergleich
zu Propranolol etwas h?her zu liegen. International und nach den Empfehlungen der Deutschen Migr?ne- und Kopfschmerzgesellschaft
gilt Valproins?ure mittlerweile als etabliertes Mittel der zweiten Wahl.
相似文献
79.
A new computed tomographic and sonographic appearance of renal metastatic melanoma is described. Bilateral cystic masses with thick walls, many with mural nodules, were noted. Sonography also demonstrated complex echopenic masses with irregularly thickened walls and mural nodules. 相似文献
80.
Red cell folate specimens were added to the Quality Assurance Program (QAP) of the Royal College of Pathologists of Australasia in 1986. The interlaboratory variation in the results of these red cell folate surveys has been persistently unsatisfactory. This study reports an investigation into the factors contributing to the wide variation of results reported in QAP surveys. Survey results were studied, replies to a questionnaire regarding methods sent to all participants were assessed and some new experimental studies were performed. Factors contributing to the poor QAP results include variation in dilution and diluent, calculation errors, failure to freeze the hemolysate prior to assay and to follow the kit manufactorer's instructions, stability of dithiothreitol, and variations in kit methods, especially in the release of bound folate by "boil" and "no-boil" methods. Photodecomposition and the form and concentration of ascorbate may also be significant. Adequate preparation of the hemolysate sample should ensure release of all protein-bound red cell folate with the reduction of all folates to a single form. Kit manufactures, users and the QAP committee can all contribute in all attempt to rectify current sources of error. 相似文献