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??Ultrasound characteristics of thyroid microcarcinoma PAN Fu-shun*??XIE Xiao-yan??LI Xiao-xi??et al. *Department of Ultrasound, the First Affiliated Hospital of Sun Yat-sun University, Guangzhou 510080,China
Corresponding author??XIE Xiao-yan??E-mail??xxy1992@live.cn
Abstract Objective To investigate the ultrasound characteristics of thyroid microcarcinoma. Methods The ultrasound imaging of thirty-four cases of thyroid microcarcinoma confirmed by surgery and pathology between January 2003 and January 2009 at the First Affiliated Hospital of Sun Yat-sun University was analyzed retrospectively. Results Thirty-two (94.1%) cases were detected as hypo-echo nodules. Twenty-eight (85.3%) cases were with irregular shape. Twenty-six (76.5%) cases were with irregular edges calcification. Blood flow was detected in 7 cases with CDFI and lymph node metastasis was found in 4 cases. Conclusion Hypo-echo nodules, regular shape, irregular edges and calcification are the ultrasound characteristic. Comprehending the characteristics may improve the pre-operation diagnosis efficacy.     

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??Study on surgical anatomy and histology of mesopancreas WU Wen-guang*??GUAN Wen-bin??LIU Yong-chen??et al. *Department of General Surgery??Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine??Shanghai 200092??China
Corresponding author??LIU Ying-bin??E-mail??laoniulyb@163.com
Abstract Objective To investigate the surgical anatomical and histological data for the mesopancreas and mesopancreatic excision. Methods The anatomic structures of the peripancreatic space??the important vessels and the retroperitoneal structures of 5 cadaveric specimens between January and December 2016 in Basic Medicine Faculty of Shanghai Jiao Tong University were observed in the study. The structures of the vessels and retroperitoneum were histological studied. Results Posterior pancreatic space was an important anatomical plane of mesopancreatic excision. Celiac trunk and superior mesenteric artery were important anatomic landmarks of mesopancreatic excision. The No.16 lymph node was the extra anatomy structure of mesopancreas. There was a natural surgical space between the adventitia and sheath of the artery. There was a great deal of nerve and lymphatic vessel distribution on and outside the arterial sheath of the peripancreatic arteries. Conclusion Mesopancreas and mesopancreatic excision have the corresponding basis of surgical anatomy. Arterial sheaths dissection strategy for improving the dissection effect has certain anatomical basis.     

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??Protection of Oddi sphincter function and its significance in endoscopic sphincterotomy LU Xin-liang*??LIANG Ting-bo. *Department of Gastroenterology, the Second Affiliated Hospital, Zhejiang University School of Medicine; Key Laboratory of Pancreatic Diseases of Zhejiang Province, Hangzhou 310009, China.
Corresponding author: LIANG Ting-bo, E-mail:liangtingbo@
zju.edu.cn
Abstract The sphincter of Oddi is a neuromuscular structure located at the junction of the bile and pancreatic ducts with the duodenum. The primary functions of the sphincter of Oddi are to regulate the delivery of bile and pancreatic juice into the duodenum and to prevent the reflux of duodenal contents restrograde infection. EST damage the integrity of the Oddi sphincter, leading to sphincter relaxation or stenosis, causing partial or complete loss of the original physiological function of the Oddi sphincter. Therefore, surgeons should strictly control the indications of EST, retain the sphincter function as far as possible, so as to provide patients with more reasonable and standardized treatment strategy.     

氨磷汀预防顺铂所致肾毒性

目的观察氨磷汀对顺铂所致肾毒性的预防性保护作用。方法随机将大鼠分成5组,即空白对照组,顺铂5mg·kg-1组,顺铂7.5mg·kg-1组,顺铂5mg·kg-1 氨磷汀200mg·kg-1组和顺铂7.5mg·kg-1 氨磷汀200mg·kg-1组,分别测定肾脏脏器系数、血尿素氮、肌酐水平,并做肾组织病理学检查。结果顺铂5mg·kg-1及7.5mg·kg-1组大鼠BUN、Cr值均明显高于对照组和加用氨磷汀组,差异有显著性(P<0.05或P<0.01);氨磷汀组肾脏脏器系数明显下降,病理分级显示氨磷汀肾损害保护率分别为61.5%和56.8%。结论氨磷汀能有效地预防顺铂所致肾毒性。     

多西环素牙周缓释凝胶的配方筛选研究

目的探索一种牙周缓释凝胶的最佳配方。方法将聚乳酸-乙醇酸共聚物(PLGA)浓度、溶剂配比和多西环素浓度按三个水平作正交设计,进行体外释放度试验,以第6天的释放百分率和凝结时间的综合分为指标,计算极差。结果按极差大小,PLGA浓度>溶剂配比>多西环素浓度,最佳配方为PLGA浓度15%,1MP:GTA=7:3,多西环素浓度为6%。结论用正交设计进行配方筛选是一种较好的试验方法。     

糖皮质激素促进β-淀粉样蛋白对海马神经元损伤的研究

目的在体外实验条件下,观察糖皮质激素是否促进β-淀粉样蛋白的神经元毒性作用。方法通过LDH释放率法检测细胞活力、TUNEL法测细胞凋亡以及LSCM测胞内Ca²⁺浓度的变化。结果单独的地塞米松(10^-8,10^-7,10^-6mol·L^-1)组或Aβ25-35(0.5μmol·L^-1)组分别作用海马神经元后,细胞活力变化不明显(P>0.05);但与同剂量的DEX、Aβ_25-35组相比,DEX+Aβ_25-35组的细胞活力明显降低(P<0.05),同时胞内Ca²⁺显著上升(P<0.05),神经元的凋亡率增加明显,细胞内出现凋亡的典型形态学特征。结论糖皮质激素可能通过促进Aβ引起海马神经元胞内Ca²⁺升高增加神经元毒性作用。     

高效液相色谱法同时测定健康人尿液中草酸和枸橼酸的含量

目的建立HPLC同时测定人尿草酸和枸橼酸含量的方法。方法采用Agilent SB C18(250mm×4.6mm,5μm)色谱柱;Agilent zorbax extend-C18(4.6mm×12.5mm,5μm)为保护柱;以0.018mol.L-1磷酸二氢钾(含0.010mol.L-1四丁基硫酸氢铵和2.69×10-5mol/L EDTA,pH2.4~2.5)为流动相;检测波长210nm;流速1.5mL.m in-1;柱温25℃;进样量20μL;以50mg/mL磺基水杨酸沉淀尿蛋白。结果草酸最低检测限为0.4μg.mL-1。线性范围为1.563~100μg.mL-1,平均回收率为97.66%,日内及日间精密度RSD<13.50%。枸橼酸最低检测限为0.8μg.mL-1。线性范围为3.125~200μg.mL-1,平均回收率为97.71%,日内及日间精密度RSD<8.25%。结论该方法简便,灵敏度高,重复性好,可用于尿路草酸钙结石成因的研究。