电针可下调缺氧缺血性脑损伤幼鼠大脑皮层硫化氢含量

目的 建立缺氧缺血性脑损伤(HIBD)大鼠模型,研究电针干预后大鼠大脑皮层硫化氢(H2S)含量及相关酶的表达变化,探讨电针治疗神经系统疾病的气体生物学机制.方法 SD大鼠32只,日龄7 d,随机分为4组,假手术(sham)对照组,sham+电针(EA)组,HIBD对照组和HIBD+EA组,每组8只.Sham+EA组、HIBD+EA组大鼠于造模次日开始予以电针刺激百会、大椎穴,30min/d,14 d为1疗程.对照组仅固定四肢,不予针刺.疗程结束次日将大鼠处死、取材.应用敏感硫电极法测定大脑皮层H2S含量;应用免疫组织化学法定位和半定量检测胱硫醚-β-合成酶(cystathionine-β-synthase,CBS)表达;应用蛋白质免疫印迹技术定量分析大脑皮层组织CBS表达.结果 HIBD对照组幼鼠脑皮层H2S含量为(26.83±4.31)nmol/mg蛋白,较sham对照组[(22.78±1.54)nmol/mg蛋白]明显升高,差异有统计学意义(P＜0.01);HIBD+EA组幼鼠脑皮层H2S含量为(18.08±2.71)amol/mg蛋白,sham+EA组为(18.91±2.78)amol/mg蛋白,较相应对照组明显降低,差异有统计学意义(P＜0.01);EA组大鼠脑皮层CBS表达较相应对照组明显降低,差异有统计学意义(P＜0.05).结论 电针可下调大脑皮层H2S/CBS体系,这可能是电针发挥对脑损伤修复作用的机制之一.

Abstract：

Objective To establish hypoxic-ischemic brain damaged (HIBD) rat model,investigate whether H2S and cystathionine-β-synthase (CBS), the key enzyme for its generation, may be a mediator of electro-acupuncture(EA) stimulation treatment for HIBD. Methods Thirty-two healthy Sprague-Dawley neonatal rats were divided into four groups randomly: sham control group ( n = 8 ); sham + EA group ( n =8); HIBD control group ( n = 8); and HIBD + EA group ( n = 8 ). HIBD rat models were established on their 7-day-old. From the next day ,rats of sham + EA group and HIBD + EA group were electric stimulated 30 min daily for 14 d,BAIHUI and DAZHUI as the acupoints. Control ones were just fixed at the same time,without acupuncture. The rats were sacrificed on the 22 nd day, one day after the treatment course. Cortical H2S concentration was measured by sensitive sulphur electrode assay. The CBS protein expression was measured by western blot analysis and immunohistochemistry for localization. Results The concentration of cortical H2S in HIBD control group was (26. 83 ± 4. 31 ) nmol/mg protein, which was significantly higher than that of sham control group[(22. 78 ± 1.54) nmol/mg protein]( P ＜ 0. 01 ). The H2 S levels in HIBD + EA group and sham + EA group were ( 18.08 ± 2.71 ) nmol/mg protein and ( 18.91 ± 2. 78 ) nmol/mg protein, respectively. Compared with corresponding control group, they were much lower( P ＜ 0. 01 ). The expression of CBS protein in rats with EA stimulation decreased in cortex compared to corresponding control group( P ＜0. 05 ).Conclusion EA can down-resulate H2S/CBS pathway. This may be one of the mechanisms of how EA contributes to the recovery of brain damage.     

内源性二氧化硫在心血管系统中的生物学意义

美国科学家关于内源性气体分子一氧化氮(NO)和一氧化碳(CO)生物学效应的研究开创了生命调节的新理论。我们近年来提出在心血管功能调节中还存在第三个气体信号分子硫化氢(H2S)[1-4],这推进了生命科学领域科学研究的进步。二氧化硫(SO2)也是一种气体小分子,以往人     

二氧化硫对实验性腹主动脉-下腔静脉分流大鼠肺动脉压力的影响

目的 研究二氧化硫( SO2)对高肺血流性肺动脉高压的调节作用.方法 25只雄性Sprague Dawley大鼠随机分为对照组(n=8)、分流组(n=8)和分流+ SO2供体组(n=9).对分流组和分流+SO2供体组大鼠行腹主动脉-下腔静脉分流术;分流+SO2供体组大鼠每天腹腔内注射亚硫酸钠／亚硫酸氢钠( Na2SO3/NaHSO3) 85 mg·kg-1·d-1,8周后以右心导管的方法测定3组大鼠肺动脉收缩压(PASP)、肺动脉平均压(PAMP)及肺动脉舒张压(PADP).结果 分流组大鼠PASP显著高于对照组大鼠[(3.91±0.66) kPa vs (2.26±0.47) kPa,P＜0.05];分流+SO2供体组大鼠PASP显著低于分流组大鼠[(3.04±0.36) kPa vs (3.91±0.66)kPa,P＜0.05];分流+SO2供体组大鼠PASP较对照组大鼠[(3.04±0.36) kPa vs(2.26±0.47)kPa,P＜0.05]显著增高.分流组大鼠PADP较对照组大鼠显著升高[(1.81±0.45) kPa vs (1.26±0.32) kPa,P＜0.05];而分流+SO2供体组大鼠PADP与分流组大鼠比较差异无统计学意义[(1.49±0.19) kPa vs (1.81±0.45) kPa,P＞0.05].分流组大鼠PAMP显著高于对照组大鼠[(2.53±0.43) kPavs(1.60±0.36) kPa,P＜0.05];分流+SO2供体组大鼠PAMP显著低于分流组大鼠[(2.01±0.23) kPa vs(2.53±0.43)kPa,P＜0.05].分流+SO2组PAMP显著高于对照组[(2.01±0.23) kPavs (1.60±0.36) kPa,P＜0.05].结论 SO2可明显降低高肺血流大鼠的PASP和PAMP.     

电针可下调缺氧缺血性脑损伤幼鼠大脑皮层硫化氢含量

Objective To establish hypoxic-ischemic brain damaged (HIBD) rat model,investigate whether H2S and cystathionine-β-synthase (CBS), the key enzyme for its generation, may be a mediator of electro-acupuncture(EA) stimulation treatment for HIBD. Methods Thirty-two healthy Sprague-Dawley neonatal rats were divided into four groups randomly: sham control group ( n = 8 ); sham + EA group ( n =8); HIBD control group ( n = 8); and HIBD + EA group ( n = 8 ). HIBD rat models were established on their 7-day-old. From the next day ,rats of sham + EA group and HIBD + EA group were electric stimulated 30 min daily for 14 d,BAIHUI and DAZHUI as the acupoints. Control ones were just fixed at the same time,without acupuncture. The rats were sacrificed on the 22 nd day, one day after the treatment course. Cortical H2S concentration was measured by sensitive sulphur electrode assay. The CBS protein expression was measured by western blot analysis and immunohistochemistry for localization. Results The concentration of cortical H2S in HIBD control group was (26. 83 ± 4. 31 ) nmol/mg protein, which was significantly higher than that of sham control group[(22. 78 ± 1.54) nmol/mg protein]( P ＜ 0. 01 ). The H2 S levels in HIBD + EA group and sham + EA group were ( 18.08 ± 2.71 ) nmol/mg protein and ( 18.91 ± 2. 78 ) nmol/mg protein, respectively. Compared with corresponding control group, they were much lower( P ＜ 0. 01 ). The expression of CBS protein in rats with EA stimulation decreased in cortex compared to corresponding control group( P ＜0. 05 ).Conclusion EA can down-resulate H2S/CBS pathway. This may be one of the mechanisms of how EA contributes to the recovery of brain damage.     

川崎病患儿血浆前白蛋白的变化及其意义

目的 观察川崎病病程中急性时相蛋白包括前白蛋白(prealbumin,PA)的变化并探讨其意义.方法 回顾总结45例川崎病患儿资料,测定患儿血浆中PA和其他炎症指标,并对其在病程中的变化进行比较分析.结果 总计45例患儿纳入研究,其中男34例,女11例;发病年龄3个月到6岁1个月,其中5岁以下41例(91.1%),占绝大多数.退热前和退热后患儿血浆PA含量分别为(32.5±28.6)mg/L、(201.5±77.3)mg/L;退热前息儿血浆PA的含量较正常参考值明显下降,退热后PA则明显回升.结论 急性负性时相蛋白PA在川崎病病程中有明显的变化;PA和CRP一样可以作为监测川崎病病情变化的重要指标.     

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目的探讨儿童体位性心动过速综合征(POTS)的临床特点。方法2008年5月至2009年10月于北京大学第一医院儿科门诊就诊,经直立试验或直立倾斜试验确诊POTS的患儿109例(POTS组),平均年龄(11.79±2.55)岁;20名健康儿童为对照组,平均年龄(11.55±3.65)岁。对每例POTS患儿详细询问病史并进行体格检查,对比分析两组儿童在生活习惯、家族史及体质特征方面特点,总结POTS组患儿发病的临床特征。结果与对照组相比,POTS组患儿在性别比例、年龄、身高、体重、平卧心率、平均动脉压方面差异无统计学意义。POTS患儿主要症状以晕厥多见(52.3%),42.2%症状发作频繁(就诊时>10次),主要症状发生季节以夏秋季多见(42.1%),发作持续时间多在1min以内(29%)。83.5%患儿发作前有诱因,发作诱因以持久站立为多见(50.5%),发作前多伴有先兆症状(78.0%),其中以头晕、黑矇、大汗、面色苍白最为常见。18例(16.5%)患儿有伴随症状,32例(29.4%)发作后仍有不适,以乏力最常见(24例)。90例(82.6%)患儿无既往疾病史,30例(27.6%)有直立不耐受家族史,46例(...     

肾上腺髓质素对高肺血流大鼠肺组织氧化应激的调节作用

目的:研究肾上腺髓质素(ADM)对高肺血流性肺动脉高压大鼠肺组织氧化应激的调节作用及其机制。方法:健康雄性SD大鼠随机分为对照组、分流组和分流+ADM组。对后2组大鼠行腹主动脉-下腔静脉分流术。8周后,对分流+ADM组大鼠皮下埋微量渗透泵持续予ADM 1.5μg·kg~(-1)·h~(-1)。继续饲养2周后,右心导管法测定平均肺动脉压(mPAP)和右心室/(左心室+室间隔)重量比值,检测肺中、小肌型动脉相对中膜厚度(RMT),比色法测定肺组织匀浆丙二醛(MDA)含量、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)活性,Western blot法检测肺组织还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶4(NOX4)的表达。结果:与对照组相比,分流组大鼠的mPAP、右心室肥厚程度以及肺中、小肌型动脉RMT均明显增加,肺组织MDA含量明显升高,T-AOC、SOD活性和GSH-Px活性均明显降低,肺组织NOX4表达明显升高。ADM使肺动脉压力明显回降,右心室肥厚程度减轻,肺血管结构改变缓解,肺组织MDA含量明显下降,T-AOC、SOD活性和GSH-Px活性明显升高,肺组织NOX4表达明显降低。结论:ADM能够抑制大鼠高肺血流性肺动脉高压和肺血管结构重构形成中的氧化应激反应,作用机制可能与其下调肺组织NOX4表达以及增强抗氧化活性有关。     

Applications of survival model in the prognostic research of orthostatic intolerance in children

Abstract Background: Orthostatic intolerance (OI) is a common disease at pediatric period which has a serious impact on physical and mental health of children. The purpose of this study was to investigate the effect of related factors on the prognosis of children with OI. Methods: The subjects are 170 children with OI, including 71 males (41.8%) and 99 females (58.2%) at the age from 6 to 17 (12.0±2.6) years old. The effect of related factors on the prognosis of children was studied by using univariate analysis. Then, the impact of children's age, symptom score, duration, disease subtype and treatment on patient’s prognosis was studied via analysis of COX proportional conversion model. Results: Among 170 cases, 48 were diagnosed with vasovagal syncope (VVS), including 28 cases of vasoinhibitory type, 16 cases of mixed type and 4 cases of cardioinhibitory; 115 cases were diagnosed with postural tachycardia syndrome (POTS) and 7 cases were diagnosed with orthostatic hypotension (OH). By using univariate analysis of Cox regression, the results showed that symptom score had a marked impact on the time of symptoms improvement of children after taking medication (P<0.05), while other univariate had not (P>0.05). Multivariate analysis by using Cox proportional hazards regression model showed that the symptom score at diagnosis had a significant effect on holding time of symptoms improvement of children after taking medication treatment (p<0.05). Kaplan-Meier curve showed that the symptom-free survival was higher in children with symptom score equal to 1 than that of children with symptom score equal to or greater than 2 during follow-up (p<0.05). Conclusions: Symptom score is an important factor to affect the time of symptom improvement after treatment for children with OI.     

一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系

目的研究一氧化碳(CO)对自发性高血压大鼠(SHR)血管平滑肌细胞(VSMC)中硫化氢(H2S)/胱硫醚γ裂解酶(CSE)的影响。方法SHRVSMC分为氯化血红素(hemin)实验组和hemin对照组,硫电极法测定培养上清中H2S含量,竞争性RTPCR法检测细胞中CSEmRNA水平。结果实验组较对照组培养上清中H2S含量显著降低[(16.03±2.15)μmol/Lvs(13.31±1.74)μmol/L],CSEmRNA水平较对照组降低[(0.17±0.04)vs(0.13±0.03)]。结论CO可下调SHR的VSMC中H2S/CSE体系。     

自主神经介导性晕厥患儿24h动态血压研究

目的:探讨自主神经介导性晕厥(neurally mediated syncope,NMs)患儿24 h动态血压监测(ambuhtory blood pressure monitoring,ABPM)中各项参数的变化,并分析24 h动态血压变化模式对NMS患儿的诊断价值.方法:2007年7月至2008年3月在北京大学第一医院儿科就诊的NMS患儿47例,其中男20例,女27例,年龄平均(11.7±2.8)岁;同时匹配对照组儿童23例,其中男12例,女11例,平均年龄(11.0±3.2)岁.进行血流动力学类型检测,将NMS患儿分为血管迷走性晕厥(vasovasal syncope,VVS)组[共16例,其中男7例,女9例,平均年龄(11.5±2.8)岁]和体位性心动过速综合征(postural orthostatic tachycardia syndrome,POTS)组[共31例,其中男13例,女18例,年龄平均(11.7±2.9)岁].比较呈现不同血流动力学模式的儿童24 h动态血压中的各项参数的变化及24 h动态血压变化模式对NMS的诊断价值.采用SPSS10.0软件进行统计.结果:POTS组的全天平均舒张压、白天平均舒张压和夜间平均收缩压比对照组分别增高了8.26%、6.66%和6.03%,两组之间差异均有统计学意义(P<0.05),;而VVS组患儿的全天平均舒张压、白天平均舒张压和夜间平均收缩压与对照组相比差异均无统计学意义(P>0.05).与对照组相比,VVS组和POTS组患儿的收缩压昼夜差值分别减少了35.92%和28.42%,两组之间差异均有统计学意义(P<0.01).VVS组和POTS组患儿的全天平均收缩压、白天平均收缩压、夜间平均舒张压以及舒张压昼夜差值与对照组相比差异均无统计学意义(P>0.05).24 h血压波动曲线结果表明,在VVS组及POTS组中,呈现"非勺型"者的比例均明显高于对照组(68.8%vs17.4%,64.5%vs17.4%,P<0.01),但"非勺型"者的比例在VVS组和POTS组中差异无统计学意义(P>0.05).24 h血压"非勺型"对于诊断NMS的敏感度为66.0%,特异度为82.6%,诊断符合率为71.4%.结论:NMS患儿的自主神经功能调节失衡,24 h动态血压变化模式对NMS具有诊断价值.