红霉素治疗喘憋性肺炎抗炎疗效观察

对红霉素治疗喘憋性肺炎抗炎疗效观察总结如下。     

峰流速仪在儿童喘息性疾病的应用评价及临床分析

本文通过观察儿童喘息性疾病发作期间使用空气压缩泵雾化吸入支气管扩张剂前后肺功能PEFR指标改善情况进行疗效评价，以确立适应儿童气道高反应性疾病药物吸入后临床评定标准。     

D-氨基酸氧化酶激活剂基因多态性与精神分裂症的关联研究

目的 探讨D-氨基酸氧化酶激活剂(D-amino acid oxidase activator,DAOA)基因rs2391191、rs947267、rs778294多态性与云南汉族人群精神分裂症发病的相关性.方法 采用聚合酶链式反应-限制性片段长度多态(PCR-RFLP)方法,对276例精神分裂症患者(患者组)和312名健康对照(对照组)DAOA基因rs2391191、rs947267和rs778294 3个位点的多态进行检测,在混合群体和性别分层群体中进行多态性与精神分裂症关联分析.结果 (1)患者组和对照组rs2391191、rs947267、rs778294 3个位点等位基因频率分布的差异无统计学意义(P>0.05).(2)患者组男性和对照组男性rs2391191等位基因的频率分布差异无统计学意义(P>0.05);患者组男性rs947267 C和rs778294 A等位基因的频率分别为0.406,0.184,对照组男性分别为0.327,0103,患者组高于对照组[校正相对风险度(OR)=1.41,95%可信区间(CI)=1.00～1.99,P=0.049;OR=1.91,95%CI=l.21～3.20,P=0.006].(3)对照组男性单倍型GAA的频率显著高于患者组男性(P=0.008).结论 DAOA基因的rs947267、rs778294位点可能与男性精神分裂症发病存在相关性,rs947267 C等位基因和rs778294 A等位基因可能是男性精神分裂症发病的危险因子;单倍型GAA可能是男性精神分裂症的保护性单倍型.

Abstract：

Objective To investigate the association between single nucleotide polymorphisms (SNPs) of D-amino acid oxidase activator (DAOA) gene and schizophrenia in Han population from Yunnan Province.Methods Two hundred and seventy-six schizophrenic patients and 312 healthy controls were recruited.Genotyping was performed using polymerase chain reaction(PCR)-restriction fragment length polymerase(RFLP).Odds ratios(ORs)and 95%confidence intervals(CIs)were calculated to evaluate the relationship between the gene polymorphisms and schizophrenia.Results (1)No significant differences of rs2391191,rs947267 and rs778294 allele frequencies were observed between the patients and controls,and the same results existed in the females.(2)In males,there was no significant difference in the allele frequency of rs2391191(P>0.05)between the two groups.The frequencies of rs947267 C allele and rs778294 A allele(0.406 and 0.184,respectively)were higher in patients compared to that in controls (0.327 and 0.103,respectively)[adjusted OR=1.41,95%CI=1.00-1.99,P=0.049;adjusted OR=1.91,95%CI=1.21-3.20,P=0.006].(3)In males,the frequency of haplotype GAA was significantly higher in controls than that in patients(P=0.008).Conclusions The DAOA gene rs947267 and rs778294 polymorphisms may be associated with schizophrenia in males.Rs947267 C allele and rs778294 A allele are possibly risk factors for schizophrenia in Yunnan Han male population.Haplotype GAA may be associated with schizophrenia as a protective factor in males.     

围产期孕妇检出罕见CisAB血型及其备血策略探讨

目的:通过免疫血清学及分子生物学方法鉴定1例孕妇罕见CisAB血型,并对其家系进行ABO血型调查,探讨疑难血型鉴定方法及罕见血型孕妇供血策略。方法:采用免疫血清学方法及家系调查初步鉴定先证者家系ABO表型,运用聚合酶链式反应序列特异性引物(PCR-SSP)法进行ABO血型基因分型,并采用Sanger测序ABO基因1～7外显子进行验证。结果:血型血清学检测发现6例家系成员中,正反定型不相符3例,先证者及其母亲、胞弟血清学试验表现为AB亚型,父亲为A型,丈夫、长子表现为O型;分子生物学：先证者ABO基因型表现为BO2,其母亲为BB,父亲为AO,胞弟为AB,丈夫、长子均为OO;CisAB基因型分型结果分别为先证者Cis-AB01/O02,母亲为Cis-AB01/B,胞弟为Cis-AB01/A。结论:对经免疫血清学ABO血型定型困难、疑难样本,可通过鉴定其家系来初步推断其血型,同时可通过分子生物学验证其ABO血型,最终寻找提供最佳的供血方案。     

小儿急性药物性肌张力不全68例误诊分析

对我院1990／2007年诊治的小儿急性药物性肌张力不全68例分析如下。 1临床资料 1．1 一般资料 本组男36例，女32例，年龄～1岁16例，～3岁19例，～6岁22例，～9岁5例，～12岁6例。