急性淋巴细胞白血病染色体分析及其临床意义

目的 研究儿童急性淋巴细胞白血病（ALL）染色体变化，探讨其临床意义。方法 通过直接法或短期培养法对40例ALL患儿骨髓细胞进行染色体分析并观察临床疗效。结果 40例ALL患儿染色体异常检出率70％（28／40），其中数目异常50％，结构异常35．7％，数目合并结构异常14．3％，核型正常组及超二倍体组患儿缓解率高于亚二倍体组及假二倍体组，具有显著统计学意义（P＜0．01），亚二倍体及t(9;22)核型为预后不良因素。结论 染色体分析对儿童ALL预后及治疗具有指导意义。     

氨甲喋呤和人血清白蛋白及单抗79结合物在动物体内的组织分布及稳定性研究

我们曾报道McAb-HSA-MTX的制备及其体外选择性细胞毒作用的实验研究,为进一步研究交联物在体内的稳定性,首先我们建立了人前B细胞(Nalm-6)白血病小鼠模型:给未经照射的BALB/c裸鼠直接接种Nalm-6细胞后无一生长肿瘤。以γ射线对BALB/c裸鼠全身照射,每7天1次,连续照射3次,每次剂量200 Gy,末次照射后第三天接种Nalm-6细胞亦未出现移植瘤。而接受相同剂量照射的裸鼠,首次照射后1周进行第二次照射,间隔3天再第三次照射,末次照射后第三天接种Nalm-6细胞,接种后7～17天,9只裸鼠中有8只出现移植瘤,经硷性磷酶桥联酶标显色技术及间接免疫荧光法鉴定,移植瘤确系Nalm-6细胞。     

SUSTAINED ENGRAFTMENT OF ALLOGENEIC BONE MARROW TRANSPLANTS IN THREE CASES OF LEUKEMIA

From 1981 through 1983, we performed three allogeneic bone marrow transplants for the treatment of leukemia. They were the first three cases ofsustained bone marrow engraftment in China. The sustained engraftment was proven by cytogenetic, isozyme, and blood group analyses. The patients are all well at the time of writing this report.The first patient was a 19-year-old female with acute monocytic leukemia. After she had attained complete remission, she was treated with a protocol including intravenous cyclophosphamide (1.8 g/m2/ day X 2) and total body irradiation (TBI) of 600 rads followed by a bone marrow transplant from her bro ther. MTX and daily intravenous placental v-glo- bulin were administered after narrow infusion. Although moderate acute and chronic graft versus host disease (GVHD) did occur, they were controlled by treatment. The second patient was a 12-year-old boy with early relapse of acute granulocytic leukemia. He received essentially the same protocol as the first patient. Acute and chronic GVHD, as well as septi- .cemia, occurred after transplanatation but were con trolled. The third patient was an adult woman with chronic granulocytic leukemia in the chronic phase. .She received essentially the same protocol except that the TBI was 700 rads and much less MTX was given. Her pht chromosome disappeared gradually after bone marrow transplantation. She had mild acute and chronic GVHD, which were controlled. Cytogenetic analysis provided evidence of a graft versus leukemia effect. We hold that intravenous .placental Y.globulin in large dosages, which had not previously been used in BMT recipients, might help to control GVHD by exerting an immunosuppres. sive effect through a feedback mechanism.