中医院校医学遗传学教学现状与课程改革探讨

医学遗传学既是一门综合性学科,又是一门实践性学科。通过对中医院校医学遗传学教学现状中存在的问题进行分析,探讨在中医院校进行医学遗传学课程改革的措施。     

丁香及其主要成分的抗凝作用

采用电刺激动脉血管所致实验性体内血栓形成方法并同步测定凝血功能变化的实验。结果表明:丁香油及丁香水提物均对大鼠血栓形成有明显抑制作用,并能明显延长血浆凝血酶原、白陶土部分凝血活酶时间而具有一定的抗凝作用。丁香油的作用强于丁香水提物。     

肾上腺髓质素与缺氧缺血性脑损伤

肾上腺髓质素是一种新发现的生物活性肽,在体内分布广泛,多种组织都有其mRNA表达,它以自分泌或旁分泌方式参与机体多种生理和病理过程的调节。具有扩张血管、降低血压和排钠利尿等作用。在中枢神经系统内作为细胞保护因子,对脑缺氧缺血性损伤起保护作用。     

针灸学选穴法则在理疗中的应用

将针灸学“阳病治阴 ,阴病治阳”法则运用于理疗 ,应用低中频电流治疗 97例腰骶部及四肢关节软组织疼痛性疾病 ,治愈 5 6例 ,显效 2 1例 ,有效 1 8例 ,无效 2例。其疗效明显优于对照组 (P<0 .0 0 1 )     

逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

中医药高等院校医学微生物学教学方法的一些思考

医学微生物学是一门重要的医学基础学科,是连接基础医学与临床医学的桥梁.中医药高等院校的学生,普遍反映的问题是该学科知识量大、内容繁杂、比较枯燥,并且与中医药的关系不大等.采用多元化的教学方法,激发学生的学习兴趣;利用多媒体教学,强化课堂教学效果;及时更新知识,加强与临床尤其是中医临床的联系;让学生积极地参与到教学活动中来,变被动学习为主动学习,为培养适应现代化建设的高素质人才打下良好基础.     

丹参酮ⅡA促进Eca109细胞凋亡转录组学研究

目的利用RNA测序(RNA sequencing,RNA-seq)进行丹参酮ⅡA促食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)Eca109细胞凋亡的转录组学研究,探讨可能的分子机制。方法丹参酮ⅡA(8μg/ml)处理Eca109细胞48 h,空白组作为对照,进行高通量转录组测序分析,筛选差异基因,分析转录组表达谱,利用GO(gene ontology)功能注释和KEGG(kyoto encyclopedia of genes and genomes)通路富集方法探讨丹参酮ⅡA促进Eca109细胞凋亡分子机制。结果转录组测序分别获得包含28,667,820、27,830,388条过滤后测序读数,构建测序文库比对分析显示,差异基因共有627条,其中7条基因表达上调,620条基因表达下调,483条基因存在不同类型可变剪接。GO分类及KEGG通路分析表明:差异表达基因涉及多种食管癌肿瘤信号通路NF-κB、Wnt、Hedgehog,且与ESCC发生发展的进程相关。结论丹参酮ⅡA促进Eca109细胞凋亡分子机制可能与下调肿瘤信号通道相关,也进一步为ESCC治疗提供新依据。