针灸促进骨折愈合的红外热像图观察

目的通过红外热像图动态观察针灸对骨折愈合的作用.方法纯种新西兰家兔52只,随机分为对照组、针刺组和艾灸组,在手术直视下用咬骨钳在双侧桡骨中下1/3处造成3mm骨缺陷的标准骨折模型,造模后第二天起进行针灸治疗.针刺组取足三里、太溪、曲池、合谷4穴,艾灸组用大杼、肾俞、阿是穴,双侧交替,分别在造模前及造模后对骨折局部肢体进行红外线热像仪扫描,并用计算机图像处理系统计算骨折局部的温度值.结果术前各组皮温无明显差别,术后第4天均有降低,第11天时两个治疗组明显高于对照组(P＜0.05),而第18天时两个治疗组明显低于对照组.结论针灸疗法具有活血化瘀功能,能改善局部微循环而促进骨折愈合.     

急诊内镜取食管巨大异物2例报告

1　病例资料和方法例 1　男 ,14个月 ,于 1997年 2月 5日始吃奶或进水后即吐 ,在当地以“胃炎”治疗无效 ,后经吞钡拍片见食管中段有 1.8ｃｍ× 2 .3ｃｍ大小的异物 ,异物上端食管扩张。其家长不明异物性质 ,2月 13日来我院求治 ,因当地无小儿胃镜 ,只能手术取异物 ,但其家长拒绝手术。经分析判断 ,认为异物可能为大枣 ,于 2月 15日经过充分准备 ,在氯胺酮麻醉下 ,试用ＸＳ - 4型胃镜为小儿取异物 ,在胃镜进入距门齿 13ｃｍ处发现异物为白团块 (表面裹有钡剂所致 ) ,用活检钳探明异物为红枣后 ,遂用活检钳和胃内手术剪剔去枣肉 ,并将枣肉冲…     

急性脊髓损伤动物模型的建立与评估

BACKGROUND: There are a variety of methods for modeling spinal cord injury at home and abroad, showing their own advantages and disadvantages, but not yet a kind of objective and standardized model meeting the clinical research. OBJECTIVE:To design a simple impactor used for establishing the spinal cord injury model, then to evaluate the parameters and stability of the Allen’s spinal cord injury model through comparing with the New York University (NYU) impactor established model. METHODS: Female rats were equivalently allotted into five groups: sham-operation group (group A), self-made impactor 5 and 10 cm groups (group B1 and B2) and NYU 1.25 and 2.5 cm groups (group C1 and C2). All groups except group A were subjected to striking at different heights. Behavioral scores were detected at 1, 3, 5 and 7 days after modeling. Nissl staining was used to observe the morphological changes after freezing section and semiquantitative analysis. RESULTS AND CONCLUSION:(1) At the same time point, the behavioral scores, damaged area ratio of spinal cord and semiquantitative analysis of dorsal horn neurons showed significant differences in the group A compared with the other groups, also between groups B1 and B2, and groups C1 and C2 (P < 0.05), but no significant differences were found between groups B1 and C1, as well as groups B2 and C2 (P > 0.05). (2) In the group A, there was a clear boundary between the grey and white matter in a butterfly shape, numerous neurons with large nucleus and obvious nucleolus, and plaques or tabby of Nissl body appeared in the cytoplasm. In contrast, in the other four groups, there were few neurons, different degrees of Gore focal, swelling, demyelinating, and vacuole of cellula could be found, and Nissl body was fuzzy or disappeared. These results administrate that the self-made impactor can establish different damage degrees of spinal cord inury models, which are close to the NYU-made models. Moreover, it can be helpful for study on spinal cord injury for reasons of good stability, low cost, and operated easily. 中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程     

HPLC法测定止嗽化痰冲剂中吗啡的含量

采用高效液相法,在YWG-C_(18)柱上,以0.01mol/L庚烷磺酸钠溶液-甲醇-乙腈-冰醋酸(70:50:5:0.1)为流动相,测定止嗽化痰冲剂中吗啡的含量。方法简捷、准确、专属性强。平均回收率为97.2％,RSD=0.67％(n=5)。     

开塞露作乙状结肠镜检的肠道准备

乙状结肠镜检查之前需清洁肠道,以往采用软皂水清洁灌肠2～3次,较为烦琐。笔者采用开塞露清洁肠道所做的乙状结肠镜检查效果均满意,具体方法如下: 镜检前1小时用针将开塞露的顶端穿孔(勿剪成毛口,以免擦伤肛管粘膜),徐徐插入肛门,将药液挤入,排便2次后,照上法再挤入开塞露一只,待患者大便干净后即可行乙状结肠镜检。 适应症:此法适用于因腹泻、便秘、或解浓血便、粘液便需做乙状结肠镜检的患者。     

抽血引起的医源性高血钾1例报告

抽血引起的医源性高血钾１例报告沈阳军区总医院北陵医院检验科（１１００３１）张俐，赵桂英，杜修云女患，因患脑血栓形成，给予快速静滴２０％甘露醇２５０ｍｌ，生理盐水５００ｍｌ加１０％氯化钾１５ｍｌ静滴。１小时后，抽时正中静脉血５ｍｌ送检。其中血清钾为９．...     

应用反意RNA探针原位杂交显示大鼠下丘脑生长抑素mRNA

原位杂交组织化学是近年发展起来的检测组织细胞中特异性DNA或RNA的技术。它是利用互补碱基对间特异性结合的特点,在已知DNA或RNA链上掺入一些标记物作为探针,去检测组织细胞中与它相应的DNA或RNA。最初一般应用DNA探针,此法比较成熟,探针也易制备,因此应用比较广泛,但由于它敏感性不高,因此在应用方面有它的局限性。RNA探针与DNA探针相比其敏感性是后者的十倍。由于单个细胞中神经肽DNA或RNA的拷贝数少,所以研究神经肽基因表达方面多用RNA探针。我们承Hoefler教     

血浆IL-18和Hcy及hsCRP与2型糖尿病大血管并发症的相关性研究

目的研究血浆白细胞介素18（IL-18）、同型半胱氨酸（Hcy）、超敏C反应蛋白（hsCRP）与2型糖尿病大血管并发症的关系。方法收集本院住院的2型糖尿病患者（50例）血浆标本为T2DM组,以健康志愿者血浆标本（20例）作为正常对照组。糖尿病患者又分为大血管并发症组（30例）及非大血管并发症组（20例）。采用ELISA法测定IL-18,高效液相色谱法测定Hcy。IL-18与Hcy、hsCRP的相关性用简单相关分析,用多元逐步线性回归分析血浆IL-18的影响因素。结果T2DM组较正常对照组血浆IL-18、Hcy、hsCRP明显升高;大血管病变组血浆IL-18、Hcy、hsCRP与非大血管病变组、正常对照组血浆IL-18、Hcy、hsCRP比较,差异有统计学意义（P〈0.05）;IL-18与Hcy、年龄、hsCRP、FPG、2hPG呈正相关;T2DM组为整体,IL-18为因变量,Hcy、hsCRP等14个因素为自变量,进行多元逐步线性回归分析,最后Hcy、FPG、hsCRP进入回归方程。结论IL-18、Hcy、hsCRP与2型糖尿病大血管并发症有关,IL-18和Hcy、hsCRP具有相关性。     

一氧化氮对骨骼肌缺血再灌注损伤中血管的作用机制

近年来发现一氧化氮（nitric oxide，N0）在微循环中具有调节血液动态平衡、维持血流恒定性、调节组织灌流及营养供给等作用。其在骨骼肌缺血再灌注时对血管的作用也引起广泛地关注。     

小儿病毒性心肌炎心率变异性分析

心率变异性（HRV）分析是一种能量分析机体交感神经和迷走神经功能的指标，是目前用以定量测定心脏自主神经功能的无创方法，本文旨在探讨病毒性心肌炎患儿HRV改变的临床意义。