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??Objective    To study the role of the CCR5 antagonists Maraviroc in the migration??chemotaxis??invasion and proliferation of human salivary adenoid cystic carcinoma. Methods    Scratches??migration??invasion and MTT assays were performed to detect the migration??chemotaxis??invasion and proliferation of SACC-83 and SACC-LM in vitro. Results    Compared with control group, the experimental group which was pretreated by CCR5 inhibitor Maraviroc had lower recuperability in cell scratch model. The number of cells which were blocked by inhibitor decreased significantly on the lower surface of the filters which were fixed in methanol in migration and invasion assays. And proliferation experiment results show that the multiplication capacity of SACC-83 and SACC-LM were obviously inhibited by Maraviroc. Conclusion    Maraviroc may be a kind of new targeted anti-tumor drugs and small-molecule antagonists of CCR5 could inhibit the development of salivary gland adenoid cystic carcinoma.     

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??Present status of immunotherapy for hepatocellular carcinoma YANG Yang*??CHEN Wen-jie??ZHANG Qi. *Department of Hepatic Surgery??Institute of Organ Transplantation??the Third Affiliated Hospital of Sun Yat-sen University??Guangzhou 510630??China
Corresponding author??YANG Yang??E-mail??yysysu@163.com
Abstract The development of biotherapy??especially immunotherapy which is the most important form of biotherapy??could meet the pressing need of better treatment modalities for Hepatocellular carcinoma (HCC) and has captured the increasing imagination of scientists and doctors. The immunotherapy??including adoptive cell transfer??tumor vaccines and checkpoint inhibi¬tors??focuses on the whole immune system besides cancer cells. And the strategies of immunotherapy to harnesses the power of the immune system improve the ability against liver cancer and then recover the immune system. Now that some clinical trails have shown that immunotherapy can effectively treat some forms of cancer and contribute to disease stabilization??live quality??and long-term survival. Therefore??cancer immunotherapy could be used more widely and be integrated them with other cancer therapies to get more therapeutic benefit.     

灌饲牙龈卟啉单胞菌对小鼠肠道炎症影响的研究

目的　研究灌饲牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）对C57bl/6小鼠结肠炎症的诱导作用。方法　将P. gingivalis ATCC33277液体增菌后备用。将15只C57bl/6小鼠适应1周后随机均分为3组，高浓度组灌饲1 × 109 CFU P. gingivalis，低浓度组灌饲1 × 108 CFU P. gingivalis，而对照组则灌饲等量无菌BHI培养液。每只小鼠每天灌饲1次，3周后处死小鼠，采集结肠及脾脏组织，行HE染色观察组织学变化，并采用实时荧光定量PCR（qRT-PCR）检测结肠组织中CD3抗原（CD3 antigen，epsilon polypeptide，CD3）、受体型蛋白酪氨酸磷酸酶C（protein tyrosine phosphatase，receptor type C，B220）、黏附G蛋白偶联受体E1（adhesion G protein-coupled receptor E1，F4/80）、转化生长因子-β（transforming growth factor-β，TGF-β）及干扰素-γ（interferon-gamma，IFN-γ）的表达水平。结果　HE染色显示高浓度组小鼠结肠黏膜下结缔组织中淋巴滤泡增多，且高浓度组小鼠脾指数出现增高的趋势，但差异无统计学意义（P > 0.05）。qRT-PCR结果显示，与对照组及低浓度组相比，高浓度组小鼠的结肠组织中B220及TGF-β的表达水平显著增高（P < 0.05），其余指标表达水平差异无统计学意义（P > 0.05）。结论　P. gingivalis可诱导结肠炎症，从而增加牙周病患者对消化系统疾病的易感性，且其可能与牙周病病情的严重程度相关。     

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??Objective    To systematically evaluate the effect of iRoot SP and AH Plus paste in the treatment of root canal. Methods    Online databases including PubMed??EMBASE??the Cochrane Library??Web of Knowledge??and Chinese databases ??Wanfang database??VIP and CNKI?? were searched systematically up to February 2017. Randomized controlled trials and high-quality comparative studies were considered eligible for inclusion. Two qualified reviewers reviewed the original articles??evaluating qualities of articles??and extracting data independently. After heterogeneity test??Meta-analysis was performed. Results    In total??seven original clinical controlled trials were included??containing 493 teeth. iRoot SP paste could improve the immediate root canal quality ??OR=0.30??95%CI????0.10 ~ 0.85?????reduce the rate of postoperative pain ??OR=0.23??95%CI????0.06 ~ 0.89?????and decrease the apical leakage ??WMD=-0.13??95%CI????-0.22 ~ -0.05????. Conclusion    iRoot SP paste in root canal therapy can significantly alleviate pain??and the long-term curative effect is good??has better biological compatibility??which is an ideal root canal filling material. Because of the small number of the literatures included??which are all in China??it is necessary to include large sample??multicenter??randomized??double-blind??placebo-controlled trails to further confirm the clinical effect.     

数字化软件辅助设计在治疗偏颌畸形患者中的应用效果研究

目的　评价数字化软件辅助设计在治疗偏颌畸形患者中的应用效果。方法　选择2016年9月至2018年9月于中国医科大学附属口腔医院口腔颌面外科就诊的偏颌畸形患者18例。所有患者术前完成三维CT检查和牙列石膏模型的光学扫描，将数据分别以DICOM格式和STL格式传送至数字化工作站。在数字化软件中构建坐标系，重建上下颌骨、血管和神经束，并完成正颌外科的手术模拟预测，最后设计并打印患者上颌骨的截骨导板、复位导板和终末咬合导板。所有患者在术后6个月到口腔颌面外科门诊复查并行上下颌骨三维CT检查。利用数字化软件测量术后6个月患者两侧上颌第一磨牙点和尖牙点到眶耳平面和冠状平面的距离，计算非对称率；测量上中切牙点和颏顶点到正中矢状面距离作为术后效果的评价指标。结果　术后6个月患者两侧上颌第一磨牙点到眶耳平面和冠状平面距离的非对称率分别为（1.8 ± 1.0）%和（3.2 ± 1.7）%；两侧上颌尖牙点到眶耳平面和冠状平面距离的非对称率分别为（1.0 ± 0.8）%和（11.0 ± 4.8）%；上中切牙点和颏顶点到正中矢状面距离分别为（0.84 ± 1.05）mm和（1.49 ± 1.23）mm。所有患者均对面型表示满意。结论　数字化软件的模拟设计能够提高手术安全性，确保术中操作的精度，提高患者术后美学效果。因此，数字化软件辅助设计在治疗偏颌畸形患者中的应用具有重要的临床意义。     

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??Diagnosis in thyroid nodules with coexistent chronic autoimmune Hashimoto thyroiditis by shear wave elastography LIANG Jin-yu*??LIU Bao-xian??WANG Wei??et al. *Department of Medical Ultrasonics??Institute of Diagnostic and Interventional Ultrasound??the First Affiliated Hospital of Sun Yat-sen University??Guangzhou 510080,China
Corresponding author: WANG Wei??E-mail??wangw73@mail.sysu.edu.cn
Abstract Objective To assess the diagnostic performance of shear wave elastography (SWE) in the differentiation of thyroid nodules with coexistent Hashimoto thyroiditis??HT??. Methods From January 2013 to June 2014??conventional ultrasound (US) and SWE were performed on 297 suspicious thyroid nodules of 260 patients with histological confirmation. 73 patients with 83 nodules were included in the HT group. Results SWE value in the maglignant nodule was significant higher than that in the benign one and surrounding parenchyma (64.4±42.8??kPa vs??26.8±15.3??kPa vs??17.7±5.3??kPa, P<0.05). SWE value in HT group of malignant module was significantly higher than that of benign one and surrounding parenchyma. In the benign and malignant nodules??SWE value between HT and non-HT groups showed no significant differences (P>0.05). SWE value of surrounding parenchyma in HT group was higher than that in non-HT group and had positive correlation with TPO-Ab(r=0.307, P<0.05). Optimal value of SWE to diagnose malignancy was 39.1kPa with sensitivity, specificity and accuracy 68.9%,91.7% and 82.9%, respectively. Conclusions 39.0 kPa of SWE value showed good diagnostic performance in the differentiation of malignant and benign thyroid nodules with coexistent HT.     

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??Objective    To provide evidence for temporomandibular joint ??TMJ??prostheses design and size chosen via three-dimensional ??3D??measurements of CT data and cluster analysis of the Chinese. Methods    CT data from 448 adults from East China with normal TMJs were recruited and reconstructed by Proplan CMF 1.4 software. Thirteen parameters of the TMJ fossa and condyle-ramus units were performed by three-dimensional measurements. The data were analyzed statistically and hierarchical cluster analyses were performed to determine the size design. Results    The anatomical database of Chinese TMJ was established. There were significant differences in the 13 TMJ measurement parameters between the males and females. The glenoid fossa was grouped into 3 clusters?? and the condyle-ramus units were grouped into 4 clusters. Discriminant analyses were capable of correctly classifying 97.24% of the glenoid fossae and 94.98% of the condyle-ramus units. Conclusion    Three-dimensional measurements and cluster analysis of Chinese TMJ osseous morphology can provide an anatomical reference for prosthesis size design of Chinese TMJ replacement.     

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??Abstracts??Objective Different diagnostic methods of biliary atresia ??BA?? and infantile intrahepatic cholestasis disease were investigated in order to find a simple?? fast??practical??economic and non invasive differential diagnostic method. Methods A total of 584 cases of infantile cholestasis were collected from May 2006 to June 2012 for persistent jaundice??pale yellow or white shit who lived in Department of Pediatric Digestion and Infection?? Tongji Hospital?? Tongji Medical College?? Huazhong University of Science & Technology??HUST??. Seven methods including clinical diagnosis??blood biochemical tests?? liver and gallbladder ultrasonography?? dynamic continuous duodenal liquid bile check?? nuclide hepatic imaging?? magnetic resonance imaging and histology were applied for differential diagnosis and the results were analyzed. Results The correctness of clinical diagnosis method was 74.5%?? sensitivity 81.6%??specificity 69.9%?? liver size: 49.0%??89.0%?? and76.9%?? stool color:83.2%??96.1% and96.7%??blood total bilirubin:63.0%??93.1%and91.2% ?? serumγ-GT:79.7%?? 71.1%and78.7% ?? dynamic duodenal liquid color check:93.3%??91.7% and92.7%?? bile acid of duodenal liquid:97.8%??100.0% and 100.0%??B graphy :89.7%??91.7%and94.3%??porta fibre block check:72.1%??29.4%and 68.7%??nuclide hepatic imaging :60.5%??100.0% and 100.0%??MRCP:88.3%??96.5%and94.4%??liver pathology :97.4%??98.2% and 94.9%. Conclusion Differential diagnosis in 1 week can help differentiate biliary atresia from infantile intrahepatic cholestasis. B-ultrasonography and dynamic duodenal fluid test are simple?? fast practical??economic and noninvasive as differential diagnostic methods.