通识教育是中医药院校人本主义教育的重要途径

从教育理念的发展及中医药院校教学改革的实践需要出发,论述通识教育对于中医药院校学生人文精神培育的重要意义;分析开展通识教育的机遇与挑战,并结合世界通识教育发展概况和中医药院校的特征,阐述通识教育和医学人文相结合提升学生素养的基本策略。     

联合靶向小干扰RNA对疱疹病毒2感染的体外抑制作用

目的 探讨联合靶向特异性小干扰RNA(siRNA)对疱疹病毒(HSV)2的体外特异抑制作用.方法 将体外合成的靶向HSV-2编码包膜糖蛋白gB的UL27.2基因、靶向DNA结合蛋白UL29.2基因的siRNA和该两种联合靶向特异性siRNA制剂,共转染Vero细胞并感染HSV-2,观察靶基因的表达情况、Vero细胞病变、空斑减数实验和子代病毒滴度并进行各组间比较:结果特异性UL27.2siRNA、UL29.2 siRNA和联合siRNA转染Vero细胞后,均能不同程度抑制各HSV-2临床株感染所导致的细胞病变,对病毒增殖抑制率分别为63.9%、86.7%和93.3%.实时定量PCR检测各组siRNA分别对UL27.2和UL29.2两个基因的表达,结果显示UL27.2 siRNA和UL29.2 siRNA对各自的靶向基因均有抑制,而联合靶向siRNA制剂对两个基因的抑制率最高.结论 联合靶向特异性siRNA制剂能有效抑制HSV-2的感染和复制.     

医学生物化学教学初探

生物化学(以下简称生化)是医学院的主干基础课,内容多、理论抽象、知识更新快,使得教与学均有一定的难度,而生化的学习效果又直接影响到以后专业课的学习.     

老年糖尿病患者的营养状态和感染

目的了解合并感染与不合并感染的老年糖尿病患者间营养状态的差别，观察营养状态对感染的影响，以期减少感染发生率。方法运用微量营养评估表（MNA）进行评分，同时检测血清白蛋白，前白蛋白、胆固醇、甘油三酯、C-反应蛋白、糖化血红蛋白、淋巴细胞计数，测量并计算体重指数、三头肌皮褶厚度、上臂肌围。结果两组糖尿病患者间的MNA评分、上臂肌围、淋巴细胞计数、白蛋白和前白蛋白差异存在显著性（P〈0．05），感染组低于未感染组。结论老年糖尿病患者由于蛋白质营养不良，易于合并感染，而感染将进一步加重营养不良，造成恶性循环。改善糖尿病患者的营养状态对降低糖尿病患者的感染发生率有益。     

The effect of repaglinide on blood glucose and islet β-cell function in newly diagnosed type 2 diabetic patients

Objective To explore the effect of repaglinide intensive treatment on islet β-cell function and long-term control of blood glucose in newly diagnosed type 2 diabetic patients. Methods Self-control and inter-group control prospective study was conducted in 80 newly diagnosed type 2 diabetic patients who were treated with short-term repaglinide intensive treatment and islet β-cell function was assessed by 75 g oral glucose tolerance test (OGTT) before and after repaglinide treatment. The changes of △I30/△G30 ratio, blood lipid, HOMA A and HOMA B were examined. Results After treatment, in successful group, middle group and defeat group, the fasting plasma glucose levels were decreased from 8.9±1.5, 8.6±1.6,9.0±2.0 to 5.0±1.4,6.3±0. 7,6.5±0. 9 mmol/L, 0. 5 h postprandial glucose levels were decreased from (12.6±1.6, 12.6±1.5, 12.4±1.3 to 8.4±1.0, 6.8±0. 7, 8. 6±0. 9)mmol/L,and 2 h postprandial glucose levels were decreased from (13.0±1.2, 13. 1±1.3, 13. 3±1.4 to 9.2±0.9, 6.6±0. 7, 9.2±0. 9)mmol/L,respectively (all P <0. 005). The ratio of △I30/△G30 was increased froml. 69±0. 31, 1.72±0. 33, 1.79±0. 36 to 4. 47±0. 62, 4. 42±0.46,12. 00±0.46 in the three groups, respectively (P<0.05). HOMA B was significantly improved (P<0. 05), while triglycerides and HOMA A were decreased(P<0. 05). The levels of fasting blood glucose and postprandial blood glucose in 21 patients were maintained within normal range for more than six months. There were significant differences in the ratio of △I30/△G30, age, repaglinide dosage and the time of reaching target of glucose [4.47±0.62 vs. 2. 0± 0.46; 39±8 vs. 56±9; 2.0±1.5 vs. 5.0±2.5; 32.4±8.0 vs. 53.3±7.6; all P<0.05] between successful group and defeat group. Conclusions The short-term intensive treatment with repaglinide can significantly improve the early secretion phase of insulin and the islet β-cell function, reconstruct of the physiological model of insulin secretion and relieve the disease.     

皮下脂肪和胎盘的抵抗素表达及与胰岛素抵抗的关系

目的探讨抵抗素与胰岛素抵抗(IR)、肥胖和糖尿病的关系。方法于2003-032004-12选取武汉协和医院正常人腹部(n=12)、腿部(n=8)、妊娠妇女腹部(n=12)皮下脂肪组织和胎盘(n=12)为标本,以Western blot法检测抵抗素蛋白表达情况,测定血糖、血脂、胰岛素、体重、身高,计算IR指数、体重指数(BMI)、体内脂肪百分比(BF%)。结果妊娠妇女胎盘组抵抗素蛋白(67905±8441)(OD值)的表达明显高于妊娠腹部组(40718±3818)(P<0·01);正常人腹部组(39421±6087)(P<0·01)、腿部组皮下脂肪组织(14942±6706)(P<0·001);妊娠腹部组和正常人腹部组皮下脂肪组织均明显高于腿部组(P<0·001);抵抗素蛋白与BMI、IR指数、BF、空腹血糖呈正相关性。结论胎盘可分泌抵抗素,抵抗素与中心性肥胖有密切关系,可能引起IR,导致肥胖、2型糖尿病和妊娠糖尿病。     

The effect of repaglinide on blood glucose and islet β-cell function in newly diagnosed type 2 diabetic patients

Objective To explore the effect of repaglinide intensive treatment on islet β-cell function and long-term control of blood glucose in newly diagnosed type 2 diabetic patients. Methods Self-control and inter-group control prospective study was conducted in 80 newly diagnosed type 2 diabetic patients who were treated with short-term repaglinide intensive treatment and islet β-cell function was assessed by 75 g oral glucose tolerance test (OGTT) before and after repaglinide treatment. The changes of △I30/△G30 ratio, blood lipid, HOMA A and HOMA B were examined. Results After treatment, in successful group, middle group and defeat group, the fasting plasma glucose levels were decreased from 8.9±1.5, 8.6±1.6,9.0±2.0 to 5.0±1.4,6.3±0. 7,6.5±0. 9 mmol/L, 0. 5 h postprandial glucose levels were decreased from (12.6±1.6, 12.6±1.5, 12.4±1.3 to 8.4±1.0, 6.8±0. 7, 8. 6±0. 9)mmol/L,and 2 h postprandial glucose levels were decreased from (13.0±1.2, 13. 1±1.3, 13. 3±1.4 to 9.2±0.9, 6.6±0. 7, 9.2±0. 9)mmol/L,respectively (all P <0. 005). The ratio of △I30/△G30 was increased froml. 69±0. 31, 1.72±0. 33, 1.79±0. 36 to 4. 47±0. 62, 4. 42±0.46,12. 00±0.46 in the three groups, respectively (P<0.05). HOMA B was significantly improved (P<0. 05), while triglycerides and HOMA A were decreased(P<0. 05). The levels of fasting blood glucose and postprandial blood glucose in 21 patients were maintained within normal range for more than six months. There were significant differences in the ratio of △I30/△G30, age, repaglinide dosage and the time of reaching target of glucose [4.47±0.62 vs. 2. 0± 0.46; 39±8 vs. 56±9; 2.0±1.5 vs. 5.0±2.5; 32.4±8.0 vs. 53.3±7.6; all P<0.05] between successful group and defeat group. Conclusions The short-term intensive treatment with repaglinide can significantly improve the early secretion phase of insulin and the islet β-cell function, reconstruct of the physiological model of insulin secretion and relieve the disease.     

复方栝楼胶囊对兔血脂、氧化修饰低密度脂蛋白和脂质过氧化作用的影响

[目的]研究复方栝楼胶囊对兔血脂，假oxLDL、SOD和MDA的影响。[方法]采用高脂饮食饲喂法建立家兔HLP(高脂血症)模型，测定各实验组兔血清中oxLDL、MDA、TG、TC、HDL-C、LDL-C的含量和SOD活性。[结果]复方栝楼胶囊(大、小剂量)组SOD活性升高，MDA、oxLDL和LDL-c下降，与模型组比较有显著差异。[结论]复方栝楼胶囊能提高机体抗氧化能力，抑制脂质过氧化反应及LDL的氧化修饰。     

腹膜恶性间皮瘤10例误诊分析

腹膜恶性间皮瘤１０例误诊分析安徽省淮南市第三人民医院（２３２０３３）刘隽吴怀国恶性间皮瘤是一种少见的恶性肿瘤，恶性腹膜间皮瘤更是少见，占所有间皮瘤的１０％～２０％。其发病隐匿，症状无特异性，诊断困难。本文就我院收治的１０例腹膜恶性间皮瘤误诊原因作如下...     

检验专业生物化学实验教学改革探索

目的改革生物化学检验实验教学方法和手段。方法通过加强实验实训基地建设、提高师资力量、完善课内外实验教学、进行全面考核来改革实验教学。结果 2009级学生生物化学检验操作技能过关率达95%,2010级学生过关率达97%。结论检验专业学生的生物化学检验操作技能和综合能力得到了提高。