Prevalence and severity of asthma and allergies in schoolchildren in Lhasa, Tibet

BACKGROUND: The International Study of Asthma and Allergies in Childhood (ISAAC) demonstrated that large variations existed in the prevalence of symptoms of asthma, allergic rhinoconjunctivitis and eczema throughout the world and that environmental factors and lifestyle customs are major determinants of the prevalence and severity of these diseases. However, the relevant data about children living at high-altitude locations were considered to be underreported. OBJECTIVE: The ISAAC Phase III programme was carried out in Lhasa, the Tibetan Autonomous Region in China, at an elevation of 3658 m above sea level to examine the occurrence of asthma, allergic rhinoconjunctivitis and eczema in schoolchildren aged 13-14 years. METHODS: All 3196 schoolchildren in eight public junior high schools in urban Lhasa who were confirmed to be 13-14 years old were invited and participated in both written and video questionnaire investigations, among which 3190 pieces of data (49.8% of boys and 50.2% of girls) were validated and analysed. RESULTS: Among the overall observations, the prevalence of 'having ever experienced wheezing', 'current wheezing' and 'diagnosed to have asthma' was 1.4%, 0.8% and 1.1%, respectively. The prevalence of current exercise-induced asthma and current nocturnal cough was 7.1% and 4.6%, respectively. The current prevalence of allergic rhinoconjunctivitis and eczema was 5.2% and 0.4%, respectively. In addition, the prevalence of rhinoconjunctivitis symptoms during the past 12 months showed no discernable differences throughout the year. CONCLUSION: The prevalence of asthma, allergic rhinoconjunctivitis and eczema over the past 12 months was the lowest among the centres, that performed ISAAC worldwide.     

舒必利与氯丙嗪合用治疗慢性精神分裂症阴性症状的对照研究

目的 探讨舒必利与氯丙嗪及舒必利,氯丙嗪治疗慢性精神分裂症阴性症状的疗效。方法 将60例以阴性症状为主要表现的慢性精神分裂症病人随机分为舒必利合并氯丙嗪组(研究组)及舒必利组,氯丙嗪3个组。用临床疗效评定标准和BPRS量表评定疗效。结果 研究组的显效率(显进 痊愈)明显比氯丙嗪组及舒必利组高,x~2检验P<0.05提示舒必利合并氯丙嗪治疗比单一用药疗效显著。舒必利组氯丙嗪组显效率比较无显著性差异(P<0.05)。结论 舒必利合并氯丙嗪治疗慢性精神分裂症阴性症状的疗效比单一用药更好,副作用更少。     

Fas在妊娠过程中表达变化的研究

目的探讨Fas在正常妊娠过程及妊娠期高血压疾病(HDCP)中的表达变化及意义。方法分别选取正常妊娠早期和晚期及HDCP胎盘绒毛组织。采用免疫组织化学方法检测Fas表达,并用高清晰彩色病理图像分析系统对其表达进行分析。结果Fas在妊娠早期胎盘组织中极少表达,妊娠晚期胎盘组织中表达增高。HDCP胎盘组织中Fas表达明显升高,与前两组比较差异有极显著性(P<0.01)。结论孕早期滋养细胞表面Fas表达在母胎免疫耐受中发挥重要作用,孕晚期Fas表达增加可能是引起HDCP的原因之一。     

Partial characterization of a non-proteinaceous, low molecular weight antigen of Eimeria tenella

A low molecular weight (LMW) antigen of Eimeria tenella, initially identified using a murine monoclonal antibody (mAb C₃4F₁) raised against E. tenella sporozoites, was partially characterized using enzymatic degradation, solvent extraction, and immunization into various inbred lines of mice. The LMW antigen could be isolated using Folch extraction (methanol/chloroform/water) and the epitope recognized by mAb C₃4F₁ was resistant to degradation by α-amylase, pronase, and proteinase K, but was sensitive to sodium m-periodate treatment or digestion using mixed glycosidases (from Turbo cornutus). These observations suggest that the antigenic epitope recognized by mAb C₃4F₁ is carbohydrate-dependent and, based on our ability to isolate the LMW antigen by Folch extraction, the epitope probably resides on a polar glycolipid. The inability of sporozoite-immunized nude mice to elicit a serum antibody response to this molecule indicates that it acts as a T-dependent antigen. Furthermore, sporozoite-immunized male CBA/N mice (with an X-linked immunodeficiency) also failed to elicit a serum antibody response to this molecule, which is consistent with a carbohydrate antigenic epitope. We propose that this antigenic molecule be designated ET-GL1 to reflect its origin and probable structure (E. tenella glycolipid 1). Received: 30 June 1999 / Accepted: 2 December 1999     

甲襞、球结膜微循环的应急观测方法

甲襞、球结膜微循环的应急观测方法是在甲襞、球结膜微循环加权积分综合定量评价方法的基础上，保留权值为４、３的指标，同时观察血管清晰度和红细胞聚集二项指标而形成。同时提供其应急观测积分表、异常分度积分值、诊断标准，并与常规方法进行比较，证明其可以反映绝大部分流态类指标和大部分形态类指标及重要的袢周改变，没有丢失微循环重度异常的信息，保证了重度异常诊断的正确。但在大致正常、轻度异常和中度异常的诊断中应急方法部有判重的倾向，说明应急方法漏掉了一些信息，不能全面地了解微循环的改变，造成了判重的倾向。因此应急方法不能代替常规方法，只能在特定条件下应用。     

Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus

The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein.     

输尿管狭窄的应用解剖学研究

目的:探讨输尿管生理狭窄的数目、位置及狭窄的程度,为选择性CT分段扫描诊断输尿管结石提供解剖学依据.方法:解剖30例国人成人尸体60条输尿管(男40条,女20条),分段观测输尿管形态、狭窄的数目、位置和管腔内径.结果:各段输尿管形态多样,主要有四类.输尿管生理狭窄的程度依次为膀胱壁内段＞输尿管盆段＞输尿管髂血管段＞输尿管腹段.结论:输尿管生理狭窄的数目不恒定.生理狭窄的部位除膀胱壁内段外其余部位均不恒定,膀胱壁内段狭窄程度最大.     

一氧化氮与白细胞精子症不育的关系探讨

目的　探讨一氧化氮 (NO)与白细胞精子症不育的关系。方法　参照WHO标准方法 ,进行精液常规分析。采用过氧化物酶染色法检测精液中白细胞密度。用改良的低渗肿胀法检测精子细胞膜的完整性。采用镀铜镉还原荧光法 ,检测精液中NO代谢产物硝酸盐 (NO 3 )。结果　白细胞精子症不育组白细胞的密度为 (1.4 8± 0 .90 )× 10 9/L ,NO水平为 (10 3.5± 2 .0 ) μmol/L ,显著高于正常生育组的(0 .73± 0 .2 8)× 10 9/L和 (41.6± 1.8) μmol/L (P分别为<0 .0 5和 <0 .0 0 1)。精子的活动率、精子速度试验 (SVT)及精子头、尾部膜完整率 ,均明显低于生育组 (P <0 .0 1) ,而精子的死亡率则显著高于生育组 (P <0 .0 1)。结论　NO水平与白细胞密度呈正相关 ;与精子的活动率、SVT及精子头、尾膜的完整率呈负相关。表明当精液中的白细胞增高时 ,可产生过量的NO导致精子中毒受损使精子的受精能力下降     

结核分枝杆菌Ag85B与IL-12基因真核共表达质粒的构建及表达

目的 :构建结核分枝杆菌Ag85B和鼠IL 12基因的共表达载体pBud85B IL12。方法 :将结核分枝杆菌Ag85B基因和鼠IL 12基因同时克隆入含多启动子的共表达载体pBudCE4 .1中 ,构建真核共表达质粒pBud85B IL12。以pBud85B IL12转染COS 7细胞 ,通过RT PCR及ELISA方法检测目的基因的表达。结果 :在COS 7细胞中同时可检测到Ag85B和IL12的表达。结论 :pBud85B IL12共表达质粒的成功构建 ,为对其免疫原性、免疫反应性及免疫保护作用的进一步研究奠定了基础     

我院基于XML异构数据集成的开发应用及研究

基于XML的数据集成，Web Services中间件能够较容易地实现对各数据源的描述以及数据源之间的数据转换，它在应用程序和数据库之间起着接口的作用．本文结合实际医院信息工作，运用VFP9 COM模型，作了有效性的研究及应用，并对标准数据集的二次开发进行了讨论。结果表明，该方法用于医院跨网络平台、跨应用系统、异构信息集成是有效的和易于操作的。