全文获取类型
收费全文 | 1000篇 |
免费 | 76篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 34篇 |
妇产科学 | 22篇 |
基础医学 | 126篇 |
口腔科学 | 26篇 |
临床医学 | 101篇 |
内科学 | 233篇 |
皮肤病学 | 53篇 |
神经病学 | 23篇 |
特种医学 | 26篇 |
外科学 | 75篇 |
综合类 | 11篇 |
一般理论 | 1篇 |
预防医学 | 60篇 |
眼科学 | 35篇 |
药学 | 69篇 |
中国医学 | 3篇 |
肿瘤学 | 166篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 37篇 |
2021年 | 53篇 |
2020年 | 48篇 |
2019年 | 56篇 |
2018年 | 69篇 |
2017年 | 31篇 |
2016年 | 54篇 |
2015年 | 51篇 |
2014年 | 47篇 |
2013年 | 61篇 |
2012年 | 94篇 |
2011年 | 84篇 |
2010年 | 44篇 |
2009年 | 30篇 |
2008年 | 52篇 |
2007年 | 51篇 |
2006年 | 47篇 |
2005年 | 49篇 |
2004年 | 26篇 |
2003年 | 16篇 |
2002年 | 26篇 |
2001年 | 2篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1991年 | 1篇 |
1988年 | 3篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1975年 | 2篇 |
排序方式: 共有1077条查询结果,搜索用时 15 毫秒
171.
Subacute demyelinating polyradiculoneuropathy complicating Epstein–Barr virus infection in GATA2 haploinsufficiency 下载免费PDF全文
Mohamed Kazamel MD Christopher J. Klein MD Eduardo E. Benarroch MD Mrinal M. Patnaik MBBS Jennifer A. Tracy MD 《Muscle & nerve》2018,57(1):150-156
Introduction: Autosomal dominant haploinsufficiency of GATA2 causes monocytopenia and natural killer cell lymphopenia, resulting in predisposition to mycobacterial, fungal, and viral infections. Methods: Herein we report on the clinical, serologic, electrophysiologic, and pathologic evaluations of a 29‐year‐old woman with GATA2 haploinsufficiency and active Epstein–Barr virus (EBV) infection complicated by subacute painful neuropathy. Results: Nerve conduction and electromyography studies showed predominantly demyelinating sensorimotor polyradiculoneuropathy. Lumbar spine MRI showed thickening and enhancement of the cauda equina nerve roots. Serum and cerebrospinal fluid anti‐IgG and IgM EBV capsid and nucleic acid antibodies were positive. Sural nerve biopsy showed microvasculitis and an increased frequency of fibers with segmental demyelination. Intravenous immunoglobulin and steroids improved the patient's neuropathy. Conclusion: GATA2 mutation–related immunodeficiency may predispose to EBV‐associated subacute demyelinating polyradiculoneuropathy by both viral susceptibility and immune dysregulation. In patients who present in this manner, immunodeficiency syndromes should be considered when lymphomatous infiltration is excluded. Immunotherapy can be helpful. Muscle Nerve 57 : 150–156, 2018 相似文献
172.
An inhibitory control is exerted when the context in which a movement has been planned changes abruptly making the impending movement inappropriate. Neurons in the frontal eye field and superior colliculus steadily increase activity before a saccadic eye movement, but cease the rise below a threshold when an impending saccade is withheld in response to an unexpected stop‐signal. This type of neural modulation has been majorly considered as an outcome of a race between preparatory and inhibitory processes ramping up to reach a decision criterion. An alternative model claims that the rate of saccade planning is diminished exclusively when the stop‐signal is detected within a stipulated period. However, due to a dearth of empirical evidence in support of the latter model, it remains unclear how the detectability of the stop‐signal influences saccade inhibition. In our study, human participants selected a visual target to look at by discriminating a go‐cue. Infrequently they cancelled saccade and reported whether they saw the stop‐signal. The go‐cue and stop‐signal both were embedded in a stream of irrelevant stimuli presented in rapid succession. Participants exhibited difficulty in detection of the stop‐signal when presented almost immediately after the go‐cue. We found a robust relationship between the detectability of the stop‐signal and the odds of saccade inhibition. Saccade latency increased exponentially with the maximum time available for processing the stop‐signal before gaze shifted. A model in which the stop‐signal onset spontaneously decelerated progressive saccade planning with the magnitude proportional to its detectability accounted for the data. 相似文献
173.
174.
175.
Outcome of elderly patients after failure to hypomethylating agents given as frontline therapy for acute myeloid leukemia: Single institution experience* 下载免费PDF全文
Rama Nanah Kristen McCullough William Hogan Kebede Begna Mrinal Patnaik Michelle Elliott Mark Litzow Aref Al‐Kali 《American journal of hematology》2017,92(9):866-871
Outcomes of acute myeloid leukemia (AML) in elderly patients unfit for intensive chemotherapy is challenging. Hypomethylating agents (HMAs) can be effective in these patients but responses are usually short‐lived. The majority of patients will either have stable disease or progress through therapy. We hereby describe the outcome of these patients at our institution after they fail HMAs. The data on 56 AML patients at Mayo Clinic, Rochester were reviewed. Patients were considered for our study if they received HMA as frontline therapy for their AML. Out of 56 patients, 15 (27%) patients received azacitidine (AZA) and 41 (73%) received decitabine. Complete remission was found in 10 (18%), with overall response of 28% and median response duration of 10 months. Thirteen (81%) out of 16 responders relapsed. Therefore 53 patients were included in the primary or secondary failure analysis with a median overall survival (OS) of 2 months after the date of failure. Out of 53 patients, 12 (23%) received subsequent treatments. None of the 12 patients who got first salvage therapy achieved remission. Five out of the 12 patients received second salvage therapy, 2 (40%) of which achieved CR. Median OS for patients who received subsequent salvage therapies was better than those who did not receive any subsequent therapy after failing HMA (9.5 vs. 2 months, P = .0009). Outcome for patients who have primary or secondary failure is very poor. Our study provides important historical data for future novel therapies, which are sorely needed for these patients. 相似文献
176.
Daniela Barraco Sonia Cerquozzi Naseema Gangat Mrinal M. Patnaik Terra Lasho Christy Finke Curtis A. Hanson Rhett P. Ketterling Animesh Pardanani Ayalew Tefferi 《American journal of hematology》2017,92(7):640-645
Monocytosis (absolute monocyte count, AMC ≥ 1 × 109/L) might accompany a spectrum of myeloid neoplasms, other than chronic myelomonocytic leukemia (CMML). In the current study, we examined the prevalence, laboratory and molecular correlates, and prognostic relevance of monocytosis in polycythemia vera (PV). Among 267 consecutive patients with World Health Organization (WHO)‐defined PV, 55 (21%) patients displayed an AMC of ≥1 × 109/L and 18 (7%) an AMC of ≥1.5 × 109/L. In general, PV patients with monocytosis were significantly older and displayed higher frequencies of leukocytosis (81% vs. 50% at AMC ≥1 × 109/L) and TET2/SRSF2 mutations (57%/29% vs. 19%/1% at AMC ≥ 1.5 × 109/L). In univariate analysis, AMC ≥1.5 × 109/L adversely affected overall (OS; P = .004; HR 2.6, 95% CI 1.4‐4.8) and myelofibrosis‐free (MFFS; P = .02; HR 4.4, 95% CI 1.3‐15.1) survival; during multivariable analysis, significance was borderline sustained for OS (P = .05) and MFFS (P = .06). Other independent risk factors for OS included unfavorable karyotype (P = .02, HR 3.39, 95% CI 1.17‐9.79), older age (P < .0001, HR 3.34 95% CI 1.97‐5.65), and leukocytosis ≥15 × 109/L (P = .004, HR 2.04, 95% CI 1.26‐3.29). In conclusion, in the current study, we encountered a higher than expected prevalence of monocytosis in patients with PV and the mutation profile and age distribution of PV patients with monocytosis is akin to those of patients with CMML and might partly contribute to their worse prognosis. 相似文献
177.
Abstract In this study, we utilize in vivo human skin and a viable ex-vivo human skin model to investigate the effect of a commercial depilatory agent on barrier function. Tape stripping was used as a positive control. The magnitude of skin barrier was quantified by measuring transepidermal water loss values on in vivo human skin and transepithelial electrical resistance measurements and tissue histology on ex vivo skin. The susceptibility to carboxylated quantum dot penetration through ex vivo skin was investigated using fluorescent microcopy analysis of microtomed skin sections and flow cytometry to quantify quantum dot association with live epidermal cells. Results show that depilatory treatment modifies the outside-in barrier sufficiently to allow quantum dots to penetrate the stratum corneum but to a lesser extent than tape stripping. The implications of these finding are discussed. 相似文献
178.
179.
Lei T Srinivasan S Tang Y Manchanda R Nagesetti A Fernandez-Fernandez A McGoron AJ 《Nanomedicine : nanotechnology, biology, and medicine》2011,7(3):324-332
The purpose of this study was to compare the cellular uptake and cytotoxicity of targeted and nontargeted doxorubicin (DOX)-loaded poly(d,l-lactide co-glycolide) (PLGA) nanoparticle (NP) drug delivery systems in drug-resistant ovarian (SKOV-3) and uterine (MES-SA/Dx5) cancer cell lines. The cellular uptakes of DOX from nonconjugated DOX-loaded NPs (DNPs) and from HER-2 antibody-conjugated DOX-loaded NPs (ADNPs) in MES-SA/Dx5 cancer cells were higher compared to free DOX. Results also showed higher uptake of DOX from ADNPs in SKOV-3 cells compared with both free DOX and DNPs treatment. Cytotoxicity results at 10 μM extracellular DOX concentration were consistent with the cellular uptake results. Our study concludes that cellular uptake and cytotoxicity of DOX can be improved in MES-SA/Dx5 cells by loading DOX into PLGA NPs. DNPs targeted to membrane receptors may enhance cellular uptake and cytotoxicity in SKOV-3 cells. FROM THE CLINICAL EDITOR: The authors of this study compare the cellular uptake and cytotoxicity of targeted and nontargeted doxorubicin loaded PLGA nanoparticle delivery systems in drug-resistant ovarian and uterine cancer cell lines, concluding that cellular uptake and cytotoxicity of doxorubicin can be improved by the proposed methods. 相似文献
180.
Liao HX Chen X Munshaw S Zhang R Marshall DJ Vandergrift N Whitesides JF Lu X Yu JS Hwang KK Gao F Markowitz M Heath SL Bar KJ Goepfert PA Montefiori DC Shaw GC Alam SM Margolis DM Denny TN Boyd SD Marshal E Egholm M Simen BB Hanczaruk B Fire AZ Voss G Kelsoe G Tomaras GD Moody MA Kepler TB Haynes BF 《The Journal of experimental medicine》2011,208(11):2237-2249
The initial antibody response to HIV-1 is targeted to envelope (Env) gp41, and is nonneutralizing and ineffective in controlling viremia. To understand the origins and characteristics of gp41-binding antibodies produced shortly after HIV-1 transmission, we isolated and studied gp41-reactive plasma cells from subjects acutely infected with HIV-1. The frequencies of somatic mutations were relatively high in these gp41-reactive antibodies. Reverted unmutated ancestors of gp41-reactive antibodies derived from subjects acutely infected with HIV-1 frequently did not react with autologous HIV-1 Env; however, these antibodies were polyreactive and frequently bound to host or bacterial antigens. In one large clonal lineage of gp41-reactive antibodies, reactivity to HIV-1 Env was acquired only after somatic mutations. Polyreactive gp41-binding antibodies were also isolated from uninfected individuals. These data suggest that the majority of gp41-binding antibodies produced after acute HIV-1 infection are cross-reactive responses generated by stimulating memory B cells that have previously been activated by non-HIV-1 antigens. 相似文献