刺人参挥发油成分及其抗真菌活性的研究

刺人参(Oplopanax elatus Nakai.)系五加科刺参属多年生具刺灌木,主要分布于东北长白山地区,蕴藏量可观。据国外报道和我们的实验研究,其根、根茎及茎可作药用,具有补气、助阳和兴奋中枢神经等作用,有类似人参的功效。为了进一步开发和利用刺人参资源,我们对其进行较为系统的研究。本文报告刺人参总挥发性成分的分析鉴定及其抗真菌活性试验。     

FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.     

铜蓝蛋白与恶性肿瘤

铜蓝蛋白(ceruloplasmin,Cp)是一种含铜的α⁃2球蛋白,主要由肝脏合成并分泌到血液中,含有95%以上的血浆铜,在调节氧化应激及铁稳态中起重要作用。近年来,越来越多的研究发现Cp在肺癌、卵巢癌、肝癌等多种恶性肿瘤中异常表达,可通过调节铁死亡、血管生成和影响肿瘤微环境参与肿瘤生长及转移,是潜在的致癌因子。本文就Cp与恶性肿瘤的研究进展作一综述。     

Duplication 2p and monosomy 8p in mosaicism: clinical, molecular cytogenetic and molecular markers of a unique case

We report on a female patient, with a de novo mosaicism for a structural rearrangement producing trisomy 2p21-->pter and monosomy 8p21-pter. GTG bands and fluorescence in situ hybridization (FISH) in lymphocytes identified: mos 46,XX,der(8)(8qter-->8p21::p21::2p21-->pter),9qh +[52]/ 46,XX,9qh+[82]. Fibroblasts showed the same cell lines in 15 and 12 cells respectively. DNA profiling with fourteen autosomal STR markers, did not reveal a chimerism status in our patient. She did not present the classical phenotype described for trisomy 2p and for monosomy 8p probably due to approximately 60% of the patient's cells being normal. The abnormality probably arose in a very early stage of development during the first post-fertilization divisions with a non-sister chromatid exchange event between chromosomes 2 and 8 producing three cellular clones: a normal clone, one with trisomy 2p and monosomy 8p and a third with monosomy 2p and trisomy 8p. Only the first two cell lines were found in both lymphocytes and fibroblasts of hypopigmented skin; the third may have been lost or limited to other tissues.     

推拿按揉法加背俞穴埋线治疗变应性鼻炎临床研究

目的:探讨推拿按揉法加背俞穴埋线治疗变应性鼻炎的临床效果。方法:选取2017年6月~2019年5月收治的变应性鼻炎患者88例,随机分为对照组和观察组各44例,均给予服用枸地氯雷他定,观察组在此基础上给予推拿按揉法加双侧背俞穴埋线治疗,比较两组临床疗效、治疗前后鼻部症状和体征积分,IgE、TNF-α、IL-4、IL-12水平及临床复发率。结果:观察组总有效率为93.18%,高于对照组的79.55%(P<0.05);观察组鼻部症状体征积分改善程度优于对照组(P<0.05);观察组IgE、TNF-α、IL-4、IL-12水平改善程度优于对照组(P<0.05);观察组复发率为4.70%,低于对照组的41.19%(P<0.05)。结论:推拿按揉法加背俞穴埋线治疗变应性鼻炎可显著减轻临床症状和体征,改善患者炎性因子水平,并有助于降低复发率。