克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

缺血性卒中急性期外周血白细胞动态变化观察

目的:探讨脑梗死后24 h内外周血白细胞动态变化的规律及白细胞变化与发病时间的关系。方法:纳入急性脑梗死患者200例,记录外周血白细胞及亚型细胞变化、发病时间等临床资料,分析外周血白细胞变化与发病时间的关系。结果:200例患者中白细胞计数升高52例(26%),中性粒细胞计数升高及淋巴细胞计数减少者分别为86例(43%)和39例(19.5%)。根据发病时间的将所有患者分为4组:0~3 h组,3~5 h组,5~8 h组和8~24 h组,4组间外周血白细胞总数、中性粒细胞计数和中性粒细胞比例、淋巴细胞比例差异有统计学意义(P0.05);0~3 h组白细胞总数和中性粒细胞数明显低于其他3组(P0.05);8~24 h组中性粒细胞比例高于0~3 h组和5~8 h组(P0.05),8~24 h组淋巴细胞比例低于0~3 h组和5~8 h组(P0.05)。结论:脑梗死急性期外周血白细胞变化与发病时间可能有关。     

Laparoscopic Excision of a Renal Subcapsular Abscess Presenting as a Subcapsular Haematoma

Renal subcapsular abscess is a very rare entity that is defined by a suppurative process localized to a space between the renal capsule and the renal parenchyma. The pathogenesis and aetiology of this entity remain speculative. To our knowledge, only five cases have been reported in the English literature. We describe a 74-year old woman with renal subcapsular abscess treated with laparoscopic removal and do a review of the literature.     

Eruptive vellus hair cysts: an effective extraction technique for treatment and diagnosis

BACKGROUND: Eruptive vellus hair cysts are uncommon developmental anomalies of vellus hair follicles that are observed in young patients. Two patients were evaluated for asymptomatic flesh-coloured papules appearing on the chest, abdomen and axillae. Lesions of both patients were diagnosed histologically as eruptive vellus hair cyst, which is a disorder with no standard treatment option. OBJECTIVE: To introduce an alternative treatment and diagnostic technique for eruptive vellus hair cysts and to evaluate this technique's effectiveness histopathologically. MATERIALS AND METHODS: We treated the lesions by using cautery and a standard dissecting forceps under topical anaesthesia. After puncturing the overlying skin in the centre using the sharp-tipped cautery point, we grasped the base of the cyst using a standard dissecting forceps and we extracted the cysts out. In patient 1, extracted cysts were sent for histopathological examination; in patient 2, microscopic examination of the extracted material was performed in potassium hydroxide preparations. RESULTS: Over 150 cysts were extracted with this technique in two patients. The technique was simple, quick and tolerated well. No lesion recurrence was observed after 4 months. On histopathological examination, extracted cysts were observed to have cyst walls. In patient 2, the diagnosis was made within minutes by microscopic examination of the potassium hydroxide preparations of the extracted material. CONCLUSION: Our extraction technique is a simple and effective treatment option for these cysts. It can be regarded as curative since the cyst walls were also extracted. Microscopic examination of the potassium hydroxide preparation of the extracted cysts suggests that this technique can also be used as a simple diagnostic procedure.     

人白细胞介素4剪接变异体的克隆及其在大肠杆菌中的表达

目的:获取一株重组人白细胞介素4选择性剪接变异体(IL-482)蛋白的高效表达菌株,为研究变态反应性疾病的发病机制以及阻滞IL-4的生物学效应奠定基础。方法:分离人外周血琳巴细胞,提取mRNA,RT- PCR获得人IL-482基因后,克隆入大肠杆菌表达载体,构建高效表达人IL-482的重组菌株。结果:成功构建了人IL-482的高效表达菌株(TNFHis-hIL-482),序列测定与文献报道一致。SDS-PAGE分析显示此菌株所表达的目的蛋白产物的相对分子质量为3．4×104,与预期结果相符。光密度扫描重组hIL-482蛋白质占细菌总蛋白的30％。结论:在大肠杆菌中成功地表达了hIL-482基因。     

骨髓间充质干细胞对大鼠肺组织胶原的影响

目的:观察骨髓间充质干细胞(MSCs)对博来霉素(BLM)引起的肺损伤大鼠肺组织胶原的影响,初步探讨其作用机制。方法:将SD大鼠MSCs异体注入受体大鼠体内,2周后观察肺组织病理学变化,同时检测肺组织羟脯氨酸含量及转化生长因子β1(TGFβ1)、血小板源性生长因子-A、B(PDGF-A,B)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)mRNA的变化。结果:MSCs的植入可使增生的大鼠肺泡间隔、基质胶原及Ⅰ、Ⅲ型胶原明显降低,使升高的肺组织羟脯氨酸及TGFβ1、PDGF-A,B、及IGF-ⅠmRNA的表达显著降低。结论:MSCs可减少肺损伤大鼠肺组织中的胶原含量,此作用与MSCs降低了多种促纤维化细胞因子的表达有关。     

丙酮酸乙酯对脓毒症小鼠血清Leptin及乳酸／丙酮酸比值、ALT和UA的影响

探讨丙酮酸乙酯(Ethyl Pyruvate,EP)对脓毒症小鼠血清Leptin、乳酸/丙酮酸比值、丙氨酸氨基转移酶(ALT)和尿酸(UA)水平的影响.建立小鼠脓毒症模型,90只昆明小鼠随机分为:假手术组(Sham组)、林格氏液组(RLS组)、EP处理组(REPS组),后两组又设立15 min、1 h、3 h、6 h组,每组10只,用全自动生化分析仪检测血清乳酸/丙酮酸比值、ALT、UA;RIA测定血清Leptin水平.结果表明:1、血清Leptin水平:REPS组后期显著高于RLS组(P＜0.05)2、乳酸/丙酮酸:与Sham组比较,RLS组与REPS组均显著升高(P＜0.05或P＜0.01);REPS组与RLS组比较各相同时间点之间无显著性差异.3、血清ALT:与Sham组比较,REPS组与RLS组均显著增高(P＜0.01或P＜0.05);REPS组在后期显著高于RLS组(P＜0.05).4、血清UA:与Sham组比较,RLS组先显著升高(P＜0.05)后降低,REPS组先降低后升高,后期显著高于Sham组(P＜0.05);REPS组与RLS组比较显著降低(P＜0.05).EP对脓毒症损伤小鼠肾功能具有一定的保护作用,并加重脓毒症早期肝脏损伤,同时能促进Leptin的释放.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

经皮集束电极射频消融治疗小肝癌

目的 探讨经皮集束电极射频消融(radiofrequency ablation,RFA)对小肝癌(直径≤3cm)的治疗效果。方法利用RF-2000TM肿瘤射频治疗系统,在B超引导下对21例小肝癌患者24个肿块进行经皮肝穿刺射频热凝治疗,并用B超及CT检查以了解RFA治疗效果,随访观察其复发和生存情况。结果 RFA治疗后100％(20/20)的肿块血供消失(另4个治疗前即无血供),且2,4个肿块呈完全凝固性坏死(100％)。随访6月-3年,21侧中存活19例,半年生存率100％。存活的19例中,已有5例生存半年,4例生存1年,6例生存2年,4例生存3年。结论 集束电极RFA治疗小肝癌创伤小,安全,疗效可靠。     

米铂的合成工艺改进

本研究对米铂(1)的合成工艺进行了改进。十四烷酸(2)和氢氧化钠在乙酸乙酯/纯化水混合溶剂中反应,经静置析晶得十四烷酸钠(3)。3和奥沙利铂(4)在水中反应,经静置析晶、抽滤、热纯化水淋洗和精制后得1,纯度达99.91%,总收率79%(以2计),并且将1中3和草酸的含量均降至0.05%以下。优化后的工艺对原料2的质量要求降低:同系物十至十六烷酸单杂含量不超过0.2%,各杂质含量之和不超过1.0%即可得到合格产品,后处理简单,易于工业化生产。