咀嚼肌表面电极肌电图重复性检验和参考值的建立

目的:对成年人咀嚼肌表面电极采集的肌电信号加以分析,研究下颌在6种不同运动状态下咀嚼肌肌电信号的可重复性,并取得正常参考值。方法:30名22-44岁受试者进行咀嚼肌表面电极肌电图检查,记录下颌姿势位、牙尖交错位和扣齿运动时双侧咬肌、颞肌前束和二腹肌前腹的肌电活动,每个受试者在3个不同时段各测试一次。结果:3次测试的咀嚼肌峰值电位之间无统计学差异(P>0.05)。下颌姿势位时,右侧咬肌峰值电位95%正常参考值为0-0.1,左侧为0-0.13,右侧颞肌前束为0-0.67,左侧颞肌前束为0-0.1,右侧二腹肌前腹为0-0.13,左侧二腹肌前腹为0-0.1;牙尖交错位时,右侧咬肌峰值电位95%正常参考值为0.33-2.53,左侧为0.47-2.5,右侧颞肌前束为0.23-2.23,左侧颞肌前束为0.2-3.67,右侧二腹肌前腹为0.13-1.07,左侧二腹肌前腹为0.1-0.87;扣齿运动时右侧咬肌峰值电位95%正常参考值为0.5-2.5,左侧为0.73-2.3,右侧颞肌前束为0.4-3.47,左侧颞肌前束为0.3-3.63,右侧二腹肌前腹为0.2-1.07,左侧二腹肌前腹为0.13-0.97。结论:表面电极采集的双侧咬肌、颞肌前束和二腹肌前腹的肌电活动具有一定的可重复性。     

NF-κB信号转导通路和肿瘤发生、转移关系的研究进展

大量研究表明,NF-κB信号转导通路可以促进肿瘤的发生.许多炎症因子、致癌剂、促癌剂和肿瘤微环境都可以激活NF-κB.NF-κB蛋白本身和其调控的蛋白与肿瘤的发生、增殖、抗凋亡、侵袭、血管生成和转移有关.在多种肿瘤中NF-κB都处于持续性激活状态.本文就NF-κB信号通路和肿瘤发生和转移之间关系的研究新进展做一综述.     

三种陶瓷酸蚀剂对Cerinate瓷和树脂粘结强度的影响

目的 :比较三种陶瓷酸蚀剂及不同酸蚀时间对Cerinate瓷与树脂粘结强度的影响。方法 :将Cerinate瓷试件分为 4组 ,一组不使用酸蚀剂 ,其余三组分别使用自制A型 (含 2 .5 %氢氟酸 )、B型 (含 6%氢氟酸 )及C型(含 10 %氢氟酸 )陶瓷酸蚀剂 ,分别酸蚀 3 0s～ 180s ,每 3 0s为一间隔 ,与树脂粘结后测试剪切强度。结果 :C配方陶瓷酸蚀剂在 3 0s时粘结强度最大 ,达 2 7.64MPa ,相应瓷表面形态最适于粘结。结论 :C配方 (含 10 %氢氟酸 )陶瓷酸蚀剂具有较好的临床应用价值。     

Cloning of hamster osteopontin and expression distribution in normal tissues and experimentally induced oral squamous-cell carcinoma

Osteopontin (OPN) is a non-collagenous extracellular matrix (ECM) protein expressed and secreted by several human cancers. This study investigated the expression pattern of OPN during development of oral squamous-cell carcinoma by using 7,12-dimethylbenz[a]anthracene (DMBA)-induced squamous-cell carcinomas in buccal pouch of syrian golden hamsters. We first identified the hamster OPN cDNA sequence by screening of a hamster calvariae cDNA library with a rat OPN cDNA probe. The resulting 1,449 bp of hamster OPN cDNA led to a deduced protein sequence of 305 amino acids containing several putative binding sites to integrins, CD44 receptors, calcium ions and hydroxyapatite, as well as multiple sites for phosphorylation, glycosylation and sulphation. Hamster OPN cDNA was then used as a probe to analyze the expression of OPN mRNA by Northern blot and in situ hybridization analyses of normal and malignant tissues. OPN mRNA was detected in several non-mineralized tissues as well as in mineralized tissues, but was not present in normal hamster buccal epithelium. DMBA-treated hamster buccal pouches expressed OPN mRNA as early as 4 weeks and displayed the highest level of expression at 15 weeks. The specimens treated with DMBA for 15 weeks exhibited histological features of squamous-cell carcinoma, presented microcrystalline deposits and showed OPN expression associated with malignant epithelium and tumor-associated macrophages. To summarize, our results suggest that buccal-pouch carcinogenesis of Syrian golden hamster may constitute an excellent experimental model to study the mechanisms by which OPN is associated with oral cancer pathogenesis, and to validate OPN-based therapeutic approaches to ameliorate oral cancer progression and metastasis.     

牙龈卟啉单胞菌的牙龈蛋白酶K-caspase样亚基的表达与青春期龈炎的临床相关性

目的检测并比较青春期龈炎的牙龈卟啉单胞菌（Porphyromonas gingivalis，PS）临床分离株的分泌蛋白和菌体蛋白中牙龈蛋白酶K（gingipain K，Kgp）-caspase样亚基的表达强度及酶活性，揭示Kgp与青春期龈炎之间可能存在的致病关系。方法检测并记录36例14～17岁的青春期龈炎患者的牙龈指数、龈沟出血指数及探诊深度，取龈下菌斑进行Pg的分离培养，16SrRNA PCR法鉴定。将获得的10个Pg临床分离株复苏，在对数生长期末提取分泌蛋白和菌体蛋白，测定其酶活性，并用Kgp-caspaae样亚基的单克隆抗体进行Western blot检测。Kgp的表达强度及酶活性与各牙周指数之间的相关关系用等级相关系数。结果青春期龈炎的Pg临床分离株的分泌蛋白和菌体蛋白中，Kgp-caspase样亚基的表达强度及酶活性与各牙周指数的大小呈正相关关系，差异有统计学意义（P〈0．05）。结论青春期龈炎龈下菌斑中Pg的Kgp十分复杂，存在表现为低相对分子质量形式的caapase样活性分子，这些细胞内功能蛋白分子将影响Pg和宿主间的相互作用；Kgp对青春期龈炎有一定的致病作用。     

氢氧化钙根管内封药预防根管治疗期间的急症

目的：探讨氢氧化钙（calcium hycroxide,CH)根管内封药对根管治疗期间急症（Endodontic interappointment emergencies,IAE)的预防效果。方法：对使用不同药物根管内封药IAE的发生率进行比较。结果：CMCP、PC、IPI、CH组IAE发生率分别是为22．0％、28．0％、26．0％和4．0％，有非常明显的差异，疼痛指数亦以CH组最优。结论：CH根管内封药，对IAE的发生有较好的预防作用。     

多壁纳米碳管增强义齿基托树脂的生物安全性研究

目的探讨多壁纳米碳管增强义齿基托树脂（MWCNTs／PMMA）复合材料的生物学性能。方法进行MWCNTs／PMMA复合材料制备，参照ISO7406技术报告相关标准，对MWCNTs／PMMA复合材料生物安全性进行体内及体外试验．包括细胞毒性试验、急性全身毒性试验及口腔黏膜刺激试验．评价复合材料的生物学性能。结果细胞毒性试验评价级别为1级．无明显细胞毒性作用：急性全身毒性试验未见任何急性毒性反应：口腔黏膜刺激试验未见异常组织学反应。结论MWCNTs／PMMA复合材料具有良好的生物安全性．安全无毒。     

两种口腔镍铬合金离子析出的体外实验研究

目的 研究Heraenium S与TILITE P两种口腔镍铬合金在唾液pH值和成分变化时离子析出行为。方法 将两种合金试件在pH6墙正常唾液环境中浸泡一周，再用pH4的酸性及酸性加0．1％F^-的人工唾液处理两小时，之后回到正常唾液环境中浸泡一周，观察其在处理前、后镍离子的析出和表面理化状态的改变。结果 ①在正常唾液环境中HeraeniumS合金镍离子的析出量大于TILITEP合金；②pH降低和氟化物的加入增大了两种合金镍离子的析出量，其中TILITEP合金大于HeraeniumS合金；③在短暂的pH降低和加入氟化物后再回到正常唾液环境中，TILITEP合金的镍离子析出量大于HeraeniumS合金；并且在合金表面可以观察到形态学上的腐蚀破坏。结论 口腔镍铬合金的镍离子析出受到口腔环境动态变化的影响，TILITEP合金对环境改变的敏感性曼大。     

Soluble CD14 levels in gingival crevicular fluid of subjects with untreated adult periodontitis

BACKGROUND: This study determined soluble CD14 (sCD14) levels in gingival crevicular fluid (GCF) and their potential relationship to periodontal conditions in adult periodontitis. METHODS: GCF was collected from 15 patients with untreated adult periodontitis. sCD14 levels were determined by ELISA and presented as total amount (ng/site) and concentration (microg/ml). The periodontal examination consisted of plaque index (PI), bleeding index (BI), probing depth (PD), and clinical attachment level (CAL). PD and CAL were measured with an electronic probe. RESULTS: sCD14 was detected in all 15 subjects and was found in 59% (62/105) of the sampled sites. The percentage of sites with sCD14 varied greatly, ranging from 14% to 100%. The mean total amount of sCD14 was 1.71+/-0.40, range 0.03 to 5.41 ng/site; the concentration of sCD14 was 14.04+/-4.15, range 0.16 to 51.74 microg/ml. No significant difference in clinical data was found between the sites with and without detectable levels of sCD14. However, on the basis of the individual profile of sCD14 levels, i.e., those individuals with >50% of the sites containing sCD14 and mean levels of sCD14 >5.0 microg/ml, the 15 subjects were divided into a high sCD14 group (9 subjects) and a low sCD14 group (6 subjects). Compared to the high group, the low group showed greater mean PD and a higher percentage of sites with PD > or = 5.0 mm (P <0.05). Consistent with this, sCD14 concentrations showed a negative correlation with PD (r(s) = -0.636, P = 0.0174). CONCLUSIONS: The present study shows that sCD14 levels in GCF varied greatly among subjects with untreated adult periodontitis. Individuals with higher levels of sCD14 in GCF and more sites containing sCD14 had fewer deep pockets. The negative correlation between GCF sCD14 levels and probing depth implies a crucial role of sCD14 in bacterially induced periodontal destruction. The relationship between GCF sCD14 levels and probing depth warrants further investigations.     

咽旁颞下区的外科解剖钥匙──茎突

目的研究茎突毗邻关系，为咽旁颞下区手术提供外科解剖基础。方法测量颅骨。结果茎实根部深面有颈静脉孔，两者相距约3mm。颈静脉孔前有动脉管外口，分隔两者间的骨板下缘厚约1．87mm。在颈动脉管外口前外方有蝶骨角棘，二者相距约6．5mm。角棘之外侧有颞下颌关节窝，之前方为棘孔。棘孔之前内侧有卵圆孔。结论茎突可作为咽旁颞下区的外科解剖钥匙。