银屑病患者血清抗角蛋白自身抗体对角朊细胞的作用

用人表皮吸附、消耗的方法观察了银屑病患者含有与不含抗角蛋白自身抗体的ＩｇＧ对培养角朊细胞代谢活性的影响，发现抗角蛋白自身抗体的存在对角朊细胞的增生代谢具有明显的抑制作用，与抗角蛋白单克隆抗体的作用相似，提示银屑病时抗角蛋白自身抗体在表皮的沉积可能量种继发性的保护反应，以限制病情的进展。     

获得性免疫缺陷综合征与银屑病

获得性免疫缺综合征(AIDS)是一种由于人类免疫缺陷病毒(HIV)感染所引起的严重免疫障碍性疾病.AIDS已使成千上万的患者死亡,迄今尚没有治愈的报道,同时由于该病患者的免疫功能紊乱也造成了某些其他疾患在流行病学、临床表现、治疗和预后等方     

柴清汤治疗慢性支气管炎30例疗效观察

柴清汤治疗慢性支气管炎３０例疗效观察史廷瑞，王刚（包头市蒙中医院）笔者自１９８５年始用柴清汤加减治疗慢性支气管炎患者３０例，取得了较好的疗效，介绍如下。１资料与方法本组共观察３０例，男２８例，女２例，年龄最大８１岁，最小５８岁，平均年龄为６７岁；病程...     

医疗器械产品召回制度探讨与典型案例分析

着重介绍了国内外医疗器械产品召回制度现状,并以强生血糖仪、佳腾除颤器两起召回事件为案例,阐明整个召回事件处理的过程,旨在为我国建立和完善医疗器械产品召回制度提供借鉴。     

电压依赖性钾通道抗体与获得性神经性肌强直

钾离子通道是人体神经元、心肌细胞上分布最为密集、功能最为重要的一种通道,对于细胞膜兴奋性的维持、动作电位的延缓、成簇放电的频率、神经网络的学习记忆储存等具有重要作用.     

双重金属烤瓷冠在牙列不齐中的应用

<正> 个别前牙反(牙合)造成牙列不齐,临床尤以上侧切牙为多见,可直接影响成人患者的美观、功能及面容。成人因年龄偏大等因素,正畸治疗往往效果不佳。但患者又十分苦恼,就诊时总想求得一种既快又好的方法来恢复牙列美观。对此,笔者设计了一种以铸造金属冠固位于反牙上,再在金属     

硬膜外腔注入曲马多用于剖宫产手术镇痛效果观察

曲马多是对呼吸抑制较小的镇痛药。我院于1999～ 2 0 0 0年对部分剖宫产手术患者采用硬膜外腔注入曲马多进行镇痛效果观察 ,现将结果报告如下。1　临床资料1 1　一般资料　选择ＡＳＡⅠ -Ⅱ级术前胎儿无宫内窘迫的剖宫产患者 30例 ,分为两组 ,观察组 15例 ,对照组 15例 ,年龄 2     

Eukaryocytic Expression of Human B7. 1 （CD80） and its Antileukemia Effect

Objective: To construct the human B7. 1 (CD80) eukaryocytic expressing veotor and its expression on HL60 cells to investigate the immunotherapeutic and immunoprotective effects of B7. 1 molecule on acute leukemia. Methods: ①B7. 1 gene was subcloned from the cloning vector using PCR. Both of the PCR products and eukaryocytic expressing vector pHook were digested with Apa I , Sal I and linked using T4 DNA ligase. The ligased products were transduced into DH5α.B7. 1 gene containing clones were selected by digestion with Apa I and Sal I which were further con-formed by sequencing. ②HL60 cells were transformed by B7. 1 with lipofectamine and detected by FACS.③Tumor formation, mice survival time and the immunotherapeutic and immunoprotective effects by immunization with B7. 1^ cells were evaluated. Results:①The PCR products were about 620 bp. Six clones were all digested by Apa I and Sal I to produce 620 bp gene fragment which was in the same way as B7. 1. It demonstrated that the recombinant vector had been constructed successfully. Further sequencing confirmed the validity of the construction. There was no nucleotide mutation. ②B7. 1 was availably expressed on HL60 cells. ③ B7. 1 HL60 cells delayed the growth of tumor and prolonged the survival time of leukemic mice, distinctively. So did the immunoprotection of B7. 1 HL60 cell. Conclusion: The human B7. 1(CD80) eukaryocytic expressing vector can be success-fully constructed by molecular cloned methods and can be stably and availably expressed on the membrane of B7. 1 negative acute myelocytic leukemia ( AML ) cell line HL60. Furthermore, the costimulatory molecalar vaccine has siipaficanl effection of immunotherapy and immunoprotection and gives the rationale for clinical application.     

1例亚急性四氯化碳中毒性肝病20年随访观察

本文报道1例因在生产环境中接触四氯化碳3个多月出现头晕、乏力、食欲不振等症状,以后其症状逐渐加重,并出现肝区疼痛、肝肿大(肋下2cm)及肝功能SGPT增高(136U)。发病半年后来我所就诊。既往无肝炎病史,多次查HB_sAg(一),诊断为亚急性四氯化碳中毒性肝病,经多次间断应用B族维生素、萄葡糖、肌苷等药物治疗,20年后治愈。     

SarCNU治疗荷人脑胶质瘤裸小鼠模型NHG—1的疗效观察

为探讨新型抗癌药SarCNU治疗人脑胶质瘤的疗效，采用腹腔给药，观察荷皮下和脑内胶质瘤裸小鼠模型体内肿瘤生长情况。结果：SarCNU能显著抑制肿瘤生长，延长荷瘤动物生存期。SarCNU与BCNU和VM26相比，具有更强的抑制肿瘤生长作用。