红景天胶囊治疗中医虚证100例临床报告

采用随机双盲对照法观察了红景天胶囊与绞股蓝胶囊治疗中医虚证１６０例的临床疗效，结果表明：红景天胶囊治疗明显效率为５４％，总有效率为８７％，与绞股蓝胶囊对照组比较，其疗效有高度统计学意义。红景天胶囊有确切的升高血红蛋白，高密度脂蛋白，超氧化物歧化酶及皮质醇的作用，同时能过氧化脂质，表明该药物具有益气生血，保护细胞免疫损伤，延缓和防止细胞衰老和作用．     

超声法提取绞股蓝总皂甙的工艺研究

本实验证明，超声法可以提高绞股蓝总皂甙的提取率，最佳提取工艺为超声高频提取3次，每次80min，得率为1．226％。     

恩七叶甜、恩五叶蜜与绞股蓝原变种成分分析及显著性测验

目的 :为合理开发利用药食两用植物绞股蓝提供依据 ;方法 :将恩七叶甜、恩五叶蜜两个新品种与绞股蓝原变种 (苦味 )按方差分析要求种植在相同的生态环境中给予相同的田间管理 ,同时播种和收获干全草进行成分分析 ;结果 :2个新品所含人参总皂苷、总氨基酸、维生素E、维生素B₁、维生素B₂ 分别高于绞股蓝原变种 33.3% ,34.3% ;11.8% ,4.7% ;76 % ,45 .8% ;46 % ,76 .1% ;41.2 % ,25 % ,均达到极显著水平 ;总糖分别低于绞股蓝原变种16.3% ,4.4 %。结论 :两个新品种不仅各有风味 ,适口性好 ,而且有效成分含量更高 ,应用领域更宽 ,市场需求量更大 ,医疗保健效果更好 ,更具有工业开发价值。     

RAPD在绞股蓝种类鉴别应用上的条件摸索

目的 为采用随机扩增多态性技术鉴别绞股蓝及其易混品乌蔹莓,进行RAPD条件摸索.方法 运用改进CTAB法提取七叶绞股蓝、五叶绞股蓝、乌蔹莓以及烟草基因组DNA,摸索影响RAPD的条件.结果 用改进CTAB法提取所得的DNA质量较好,均能用于RAPD扩增,并摸索出了最优RAPD扩增条件.结论 改进的CTAB法适合于这4种植物的DNA提取,摸索得到的RAPD扩增条件可用于绞股蓝种类鉴别及与其易混品乌蔹莓的区别.     

A New Triterpene Saponin from Gynostemma pentaphyllum

Objective To study the triterpene saponins from Gynostemma pentaphyllum with antitumor activities. Methods The 75% EtOH extract of G. pentaphyllum was used for isolation by silica gel column chromatography and preparative HPLC. The structures of pure compounds isolated were identified by the spectral analysis and chemical evidence. Results Two compounds were isolated and identified as 23(S)-3β,20ξ,21ξ-trihydroxy-19-oxo-21,23-epoxydammar-24-ene 3-O-α-L-rhamnopyranosyl (1→2)-[β-D-xylopyranosyl (1→3)]-β-D-arab...     

绞股蓝正丁醇提取物对快速老化小鼠学习记忆的影响及其机制

目的研究绞股蓝正丁醇部位对阿尔茨海默病(AD)模型小鼠学习记忆的影响。方法采用Morris水迷宫方法对小鼠记忆行为进行检测;采用ELISA法测定SOD、GSH-PX和MDA的含量;采用HE法染色观察对小鼠大脑海马组织的影响。结果绞股蓝正丁醇部位能改善老年痴呆小鼠的学习记忆能力;能促进SOD和GSH-PX生成,抑制MDA的生成。结论绞股蓝正丁醇部位可提高AD小鼠的抗氧化能力,从而起到防治AD的作用。     

绞股蓝的药理作用及临床治疗高脂血症研究概况

绞股蓝的主要药效成分是绞股蓝皂苷,具有非常广泛的药理作用,其在治疗高脂血症上也具有很好的疗效。本文概述了绞股蓝的药理作用及临床治疗高脂血症方面的研究概况,为绞股蓝的进一步研究开发和临床用药提供思路和参考。     

Effects of Polysaccharides from Gynostemma Pentaphyllum (Thunb.), Makino on Physical Fatigue

Background

Gynostemma pentaphyllum (Thunb.) Makino has been reported to have a wide range of health benefits in Chinese herbal medicines. Polysaccharides from Gynostemma pentaphyllum (PGP), has been identified as one of the active ingredients responsible for its biological activities. Although many pharmacological activities of PGP have received a great deal of attention, there is limited evidence for the anti-fatigue effects of PGP. The purpose of this study was to investigate the effects of polysaccharides from PGP on physical fatigue.

Materials and method

The rats were divided into four groups, with 10 animals per group: control (C), group, low-treated (LT), group, medium-treated (MT), group, and high-treated (HT), group. The C group received distilled water, while LT, MT and HT groups were given various doses of PGP (100, 200, 400 mg/kg· d). After 30 days, forced swimming test was carried out in an acrylic plastic pool, then the exhaustive swimming time of rats and some biochemical parameters related to fatigue were measured. The data obtained showed that PGP could extend the exhaustive swimming time of the rats, as well as decrease the blood lactic acid (BLA), and blood urea nitrogen (BUN), concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations.

Result

The data obtained showed that different doses of PGP could extend the exhaustive swimming time of the rats, as well as decrease the BLA and BUN concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations, which suggests that PGP had significant anti-fatigue effects on rats.

Conclusion

PGP may be of use as a potential anti-fatigue agent, but there is a need for further research on long-term use in order to show its positive effects on physical fatigue.     

绞股蓝提取物制备工艺研究

目的:研究出绞股蓝提取物,为绞股蓝产品研发提供实验数据。方法:建立绞股蓝提取物的化学评价方法;优化绞股蓝提取物的提取工艺,用化学评价方法分别对绞股蓝的乙醇浓度、料液比、提取时间、提取次数4个单因素进行考察,以L 9(34)正交表设计优化工艺,筛选出最佳提取工艺。结果:经单因素考察和正交优化得到绞股蓝提取物制备工艺为料液比为1∶8的70%乙醇,加热回流提取3次,每次45 min,绞股蓝总皂苷含量为0.1025 mg/mL,提取率为10.25%。结论:建立的化学评价方法可评价绞股蓝提取物;制备工艺能为绞股蓝产品研发提供依据。