全文获取类型
收费全文 | 649篇 |
免费 | 64篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 18篇 |
妇产科学 | 22篇 |
基础医学 | 138篇 |
口腔科学 | 22篇 |
临床医学 | 39篇 |
内科学 | 156篇 |
皮肤病学 | 49篇 |
神经病学 | 44篇 |
特种医学 | 7篇 |
外科学 | 47篇 |
综合类 | 11篇 |
预防医学 | 46篇 |
眼科学 | 16篇 |
药学 | 51篇 |
中国医学 | 7篇 |
肿瘤学 | 42篇 |
出版年
2024年 | 1篇 |
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 27篇 |
2020年 | 13篇 |
2019年 | 23篇 |
2018年 | 23篇 |
2017年 | 28篇 |
2016年 | 24篇 |
2015年 | 23篇 |
2014年 | 24篇 |
2013年 | 27篇 |
2012年 | 48篇 |
2011年 | 39篇 |
2010年 | 31篇 |
2009年 | 31篇 |
2008年 | 53篇 |
2007年 | 53篇 |
2006年 | 56篇 |
2005年 | 37篇 |
2004年 | 35篇 |
2003年 | 28篇 |
2002年 | 37篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1990年 | 5篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有719条查询结果,搜索用时 15 毫秒
1.
Mirna SuČIĆ Dubravka Boban Mirjana MarkoviĆ-glamoČak Mladen PetroveČki Matko MaruŠIĆ Boris Labar 《Medical oncology (Northwood, London, England)》1992,9(1):41-45
Cytochemical analysis of leukemic blasts from 46 patients with acute myeloblastic M2 leukemia (according to the FAB classification)
was performed before and after cytostatic therapy, and compared with findings obtained in 20 age- and sex-matched control
subjects. Cytochemical findings for myeloperoxidase (MPO), Sudan black B, acid phosphatase and alpha-naphthyl-acetate esterase
(ANAE) were related to the achievement of the first complete remission (CR),i.e. data were compared after the patients had been divided into CR and non-CR groups. The analysis clearly showed that a high
proportion of myeloperoxidase- and, to a lesser extent, Sudan black B-positive blasts before treatment may have constituted
a significantly unfavourable prognostic factor. 相似文献
2.
Gordon Lauc Annie-Pierre Seve Jean Hubert Mirna Flgel-Mrsic Werner E. G. Müller Heinz C. Schrder 《Mechanisms of ageing and development》1993,70(3):227-236
Previous studies have demonstrated the existence of nuclear carbohydrate binding proteins in a variety of mammalian cells with molecular masses of 35 000, 67 000, and 70 000 (CBP35, CBP67, and CBP70), which are associated with nuclear ribonucleoprotein (RNP) complexes. CBP35 consists of two domains, an aminoterminal portion that is homologous to certain regions of proteins of the heterogeneous nuclear RNP complex, and a carboxyl-terminal portion homologous to β-galactoside-specific lectins. CBP35 it has been proposed, like the glucose-specific lectin, CBP67, to guide RNP complexes through the nuclear pore. Here we show that the exposure of mature rats to stress induces an increase in nuclear CBP35 bound to CBP67 and retained on immobilized glucose. Nuclear extracts from the livers of old rats displayed no detectable stress response. This CBP35·CBP67 association detected in rat liver is considered with respect to the CBP35·CBP70 association recently observed in HL60 cell nuclear extracts. 相似文献
3.
Interactions between imidazoline compounds and sulphonylureas in the regulation of insulin secretion
Mirna Mourtada Colin A Brown Stephen A Smith Valerie Piercy Susan L F Chan Noel G Morgan 《British journal of pharmacology》1997,121(4):799-805
- Imidazoline α2-antagonist drugs such as efaroxan have been shown to increase the insulin secretory response to sulphonylureas from rat pancreatic B-cells. We have investigated whether this reflects binding to an islet imidazoline receptor or whether α2-adrenoceptor antagonism is involved.
- Administration of (±)-efaroxan or glibenclamide to Wistar rats was associated with a transient increase in plasma insulin. When both drugs were administered together, the resultant increase in insulin levels was much greater than that obtained with either drug alone.
- Use of the resolved enantiomers of efaroxan revealed that the ability of the compound to enhance the insulin secretory response to glibenclamide resided only in the α2-selective-(+)-enantiomer; the imidazoline receptor-selective-(−)-enantiomer was ineffective.
- In vitro, (+)-efaroxan increased the insulin secretory response to glibenclamide in rat freshly isolated and cultured islets of Langerhans, whereas (−)-efaroxan was inactive. By contrast, (+)-efaroxan did not potentiate glucose-induced insulin secretion but (−)-efaroxan induced a marked increase in insulin secretion from islets incubated in the presence of 6 mM glucose.
- Incubation of rat islets under conditions designed to minimize the extent of α2-adrenoceptor signalling (by receptor blockade with phenoxybenzamine; receptor down-regulation or treatment with pertussis toxin) abolished the capacity of (+)-and (±)-efaroxan to enhance the insulin secretory response to glibenclamide. However, these manoeuvres did not alter the ability of (±)-efaroxan to potentiate glucose-induced insulin secretion.
- The results indicate that the enantiomers of efaroxan exert differential effects on insulin secretion which may result from binding to effector sites having opposite stereoselectivity. Binding of (−)-efaroxan (presumably to imidazoline receptors) results in potentiation of glucose-induced insulin secretion, whereas interaction of (+)-efaroxan with a second site leads to selective enhancement of sulphonylurea-induced insulin release.
4.
Carbonic anhydrase IX expression predicts outcome of interleukin 2 therapy for renal cancer. 总被引:4,自引:0,他引:4
Michael Atkins Meredith Regan David McDermott James Mier Eric Stanbridge Amanda Youmans Philip Febbo Melissa Upton Mirna Lechpammer Sabina Signoretti 《Clinical cancer research》2005,11(10):3714-3721
PURPOSE: Renal cancer response to interleukin 2 (IL-2) therapy and patient survival has been correlated with tumor histology and carbonic anhydrase IX (CAIX) expression. In an effort to confirm and expand these observations, we examined CAIX expression in pathology specimens from renal cancer patients who had previously received IL-2 therapy. EXPERIMENTAL DESIGN: Paraffin-embedded tissue sections of renal cancer were immunostained with the MN-75 monoclonal antibody to CAIX and expression levels were correlated with histologic findings and clinical outcome. RESULTS: Tissue specimens were obtained from 66 patients; 27 of whom (41%) had responded to IL-2-based therapy. Fifty-eight specimens were assessed as clear cell, with 56, 33, and 4 having alveolar, granular, and papillary features, respectively. Twenty-four (36%), 31 (47%), and 11 (17%) were classified into good, intermediate, and poor prognosis groups according to the Upton pathology model. Forty-one specimens (62%) had high CAIX expression. Twenty-one of 27 (78%) responding patients had high CAIX expressing tumors compared with 20 of 39 (51%) nonresponders (odds ratio, 3.3; P = 0.04). Median survival was prolonged (P = 0.04) and survival >5 years was only seen in high CAIX expressers. In patients with intermediate pathologic prognosis, all nine responders had high CAIX expression versus 11 of 22 nonresponders. A resultant group with good pathologic prognosis alone or with intermediate pathologic prognosis and high CAIX contained 26 of 27 (96%) responders compared with 18 of 39 (46%) nonresponders (odds ratio, 30; P < 0.01) and exhibited longer median survival (P < 0.01). CONCLUSIONS: CAIX expression seems to be an important predictor of outcome in renal cell carcinoma patients receiving IL-2-based therapy and may enhance prognostic information obtained from pathology specimens. 相似文献
5.
Berlinck RG Hajdu E da Rocha RM de Oliveira JH Hernández IL Seleghim MH Granato AC de Almeida EV Nuñez CV Muricy G Peixinho S Pessoa C Moraes MO Cavalcanti BC Nascimento GG Thiemann O Silva M Souza AO Silva CL Minarini PR 《Journal of natural products》2004,67(3):510-522
Brazil is blessed with a great biodiversity, which constitutes one of the most important sources of biologically active compounds, even if it has been largely underexplored. As is the case of the Amazon and Atlantic rainforests, the Brazilian marine fauna remains practically unexplored in the search for new biologically active natural products. Considering that marine organisms have been shown to be one of the most promising sources of new bioactive compounds for the treatment of different human diseases, the 8000 km of the Brazilian coastline represents a great potential for finding new pharmacologically active secondary metabolites. This review presents the status of marine natural products chemistry in Brazil, including results reported by different research groups with emphasis on the isolation, structure elucidation, and evaluation of biological activities of natural products isolated from sponges, ascidians, octocorals, and Opistobranch mollusks. A brief overview of the first Brazilian program on the isolation of marine bacteria and fungi, directed toward the production of biologically active compounds, is also discussed. The current multidisciplinary collaborative program under development at the Universidade de S?o Paulo proposes to establish a new paradigm toward the management of the Brazilian marine biodiversity, integrating research on the species diversity, ecology, taxonomy, and biogeography of marine invertebrates and microorganisms. This program also includes a broad screening program of Brazilian marine bioresources, to search for active compounds that may be of interest for the development of new drug leads. 相似文献
6.
OBJECTIVE: The aim of the study was to assess the rate of acceptance of preimplantation genetic diagnosis (PGD) as an alternative to prenatal diagnosis in Lebanese women with previously affected children with homozygous beta-thalassemia. METHODS: Women with a previously affected child attending a nongovernmentally funded thalassemia care center between 1 June 2005 and 31 May 2007 were offered a genetic counseling session. This was followed by administering a questionnaire through direct interview. RESULTS: All 97 women approached accepted to participate in the study (100% response rate). Sixty eight per cent of women considered PGD a better alternative to prenatal diagnosis. The most important perceived advantage of PGD was the avoidance of termination of an affected pregnancy. CONCLUSIONS: PGD is an acceptable alternative to conventional prenatal diagnosis in women at risk of conceiving a child affected with beta-thalassemia. This is particularly true in countries of the Middle-East where therapeutic abortions for fetal indications are prohibited by the law and religion. Copyright (c) 2008 John Wiley & Sons, Ltd. 相似文献
7.
8.
9.
Distinctive arthritic patterns, some of which may parallel or even precede intestinal disease activity, are seen in inflammatory
bowel disease. Some spondyloarthropathies are associated with transient ileocolic inflammation. Vasculitis frequently affects
the gastrointestinal tract, predominantly manifesting with abdominal pain. In severe cases, intestinal ischemia and perforation
may occur. Various arthritides are thought to be associated with other gastrointestinal diseases, such as celiac disease and
hepatitis. The association between intestinal disease and arthritis is still being investigated. Interactions between the
inflammatory intestinal cells and inflamed synovial cells have been demonstrated. Certain intestinal bacteria such as Klebsiella pneumoniae are suspected to play a role as triggers for the development of arthropathies. Genetic factors, especially human leukocyte
antigen associations, are also being increasingly investigated for better characterization of the types of arthritis and possible
prognostic implications. Various therapies, including nonsteroidal anti-inflammatory drugs, used to treat rheumatologic diseases
have the potential to cause gastrointestinal complications. 相似文献
10.
Ismael Nilo Lino de Queiroz Ana Luíza Gomes Quinderé José Ariévilo Gurgel Rodrigues Edfranck de Sousa Oliveira Vanderlei Natássia Albuquerque Ribeiro Renata Line da Conceição Rivanor Kátia Alves Ribeiro Chistiane Oliveira Coura Karuza Maria Alves Pereira Hellíada Vasconcelos Chaves Mirna Marques Bezerra Ianna Wivianne Fernandes de Araújo Norma Maria Barros Benevides 《Inflammation research》2015,64(12):971-982