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1.
Intracellular pH, H ion flux and H ion permeability coefficient in bullfrog toe muscle 总被引:1,自引:1,他引:1
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K T Izutsu 《The Journal of physiology》1972,221(1):15-27
1. The dimethyloxazolidinedione (DMO) technique was used to estimate intracellular pH (pH(i)) in bullfrog toe muscles incubated in vitro. The control value of pH(i) was 7.16 + +/- 0.01 (S.D.).2. pH(i) was affected by changes in P(CO2) and external bicarbonate ion concentration ([HCO(3) (-)](0)). At a given P(CO2), decreasing the external [HCO(3) (-)] was more effective in lowering pH(i) than increasing the external [HCO(3) (-)] was in increasing pH(i).3. On the assumption that the changes in pH(i) were due to hydrogen ion [H(+)) movements across the membrane, a H(+) flux of 10(-13) mole/cm(2). sec was calculated. The corresponding H(+) permeability coefficient was 10(-3) cm/sec.4. The variability of the tissue CO(2) buffer value was examined. 相似文献
2.
The relationship between physical stability of freeze-dried cakes and protein stability during storage was studied using -galactosidase as a model protein and inositol as an excipient. Amorphous samples freeze-dried from solutions containing the enzyme and various concentrations of inositol in sodium phosphate buffer (50 mM, pH 7.4) were stored for 7 days over P2O5 at 40 to 70°C. Structural collapse and inositol crystallization were observed in some of the samples, depending on the formulation and storage temperature. The physical stability of freeze-dried samples was also studied by differential scanning calorimeter (DSC). Inositol showed a protein-stabilizing effect when its amorphous form was retained during storage, regardless of structural collapse. However, crystallization of inositol during storage removed its stabilizing effect. Addition of water-soluble polymers such as dextran, Ficoll and carboxymethyl cellulose sodium salt (CMC-Na) preserved activity of the enzyme by preventing inositol crystallization. 相似文献
3.
Y Kanda K Izutsu H Hirai H Sakamaki T Iseki Y Kodera S Okamoto H Mitsui K Iwato N Hirabayashi T Furukawa A Maruta M Kasai Y Atsuta N Hamajima A Hiraoka K Kawa 《Leukemia》2004,18(5):1013-1019
The effect of graft-versus-host disease (GVHD) on relapse incidence and survival has been analyzed in several studies, but previous studies included heterogeneous patients. Therefore, we analyzed the data of 2114 patients who received unmanipulated bone marrow graft from an HLA-identical sibling donor with a GVHD prophylaxis using cyclosporin A and methotrexate. Among the 1843 patients who survived without relapse at 60 days after transplantation, 435 (24%) developed grade II-IV acute GVHD. Among the 1566 patients who survived without relapse at 150 days after transplantation, 705 (47%) developed chronic GVHD. The incidence of relapse was significantly lower in patients who developed acute or chronic GVHD, but disease-free survival (DFS) was significantly inferior in patients who developed acute GVHD. A benefit of 'mild' GVHD was only seen in high-risk patients who developed grade I acute GVHD. The strongest association between GVHD and a decreased incidence of relapse was observed in patients with standard-risk acute myelogenous leukemia/myelodysplastic syndrome. In conclusion, the therapeutic window between decreased relapse and increased transplant-related mortality due to the development of GVHD appeared to be very narrow. 相似文献
4.
Hiroyuki Ito Kiyoko Ito Mahika Tanaka Mayumi Hokamura Mari Tanaka Eiji Kusano Jiro Kondo Takuma Izutsu Suzuko Matsumoto Hideyuki Inoue Shinichi Antoku Tomoko Yamasaki Toshiko Mori Michiko Togane 《Internal medicine (Tokyo, Japan)》2022,61(9):1309
Objective Diabetes is recognized as an underlying disease of constipation. However, the prevalence of constipation varies according to the diagnostic criteria applied. We investigated the prevalence of constipation based on the new guideline for constipation in Japanese patients with type 2 diabetes and examined the relationship with the clinical background, including diabetic vascular complications. Methods Questionnaire surveys including items concerning the diagnosis and treatment status of constipation were administered to 410 patients with type 2 diabetes. Results Although 29% of the patients considered that they had experienced constipation (self-judged), only 14% had consulted a physician about constipation. The prevalence of chronic constipation based on the guideline was 26%. After including laxative users, constipation was finally found in 36%. Despite the use of laxatives (n=81), 51% of the patients were still diagnosed with chronic constipation. Patients with constipation (chronic constipation or laxative use) were significantly older and had a longer duration of diabetes than those without constipation. The body mass index (BMI) of patients with constipation (24.9±3.8 kg/m2) was significantly lower than that of those without constipation (26.3±4.6 kg/m2). Diabetic neuropathy (49% vs. 32%) and coronary heart disease (CHD) (27% vs. 13%) were significantly more frequent in the patients with constipation than in those without constipation. A multivariate logistic regression analysis revealed that gender, BMI, diabetic neuropathy, insulin use, and CHD were significantly associated with constipation. Conclusion An accurate diagnosis of constipation is desirable in patients with type 2 diabetes because constipation is independently associated with CHD. 相似文献
5.
Akihisa Kawajiri Shigehisa Kitano Akiko Miyagi Maeshima Yoshihiro Inamoto Kinuko Tajima Tomonari Takemura Takashi Tanaka Ayumu Ito Keiji Okinaka Saiko Kurosawa Sung‐Won Kim Hirokazu Taniguchi Chitose Ogawa Koji Izutsu Noboru Yamamoto Takahiro Fukuda 《European journal of haematology》2019,103(6):578-587
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7.
Masuda A Nakamura K Izutsu K Igarashi K Ohkawa R Jona M Higashi K Yokota H Okudaira S Kishimoto T Watanabe T Koike Y Ikeda H Kozai Y Kurokawa M Aoki J Yatomi Y 《British journal of haematology》2008,143(1):60-70
Autotaxin (ATX) is a tumour cell motility-stimulating factor originally isolated from melanoma cell supernatants. ATX is identical to lysophospholipase D, which produces a bioactive lipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine. ATX is overexpressed in various malignancies, including Hodgkin lymphoma, and ATX may stimulate tumour progression via LPA production. The present study measured the serum ATX antigen levels in patients with haematological malignancies using a recently developed automated enzyme immunoassay. The serum ATX antigen levels in patients with B-cell neoplasms, especially follicular lymphoma (FL), were higher than those in healthy subjects. Serum ATX antigen levels in FL patients were associated with tumour burden and changed in parallel with the patients' clinical courses. The serum ATX antigen levels were little affected by inflammation, unlike the soluble interleukin-2 receptor and beta2-microglobulin levels. As expected, the plasma LPA levels in FL patients were correlated with the serum ATX antigen levels. Given that leukaemic tumour cells from FL patients expressed ATX, the shedding of ATX from lymphoma cells probably leads to the elevation of serum ATX antigen levels. Our results suggest that the serum ATX antigen level may be a promising and novel marker for FL. 相似文献
8.
Tomohiro Aoki Koji Izutsu Ritsuro Suzuki Chiaki Nakaseko Hiroshi Arima Kazuyuki Shimada Akihiro Tomita Makoto Sasaki Jun Takizawa Kinuko Mitani Tadahiko Igarashi Yoshinobu Maeda Noriko Fukuhara Fumihiro Ishida Nozomi Niitsu Ken Ohmachi Hirotaka Takasaki Naoya Nakamura Tomohiro Kinoshita Shigeo Nakamura Michinori Ogura 《Haematologica》2014,99(12):1817-1825
The prognosis of patients with primary mediastinal large B-cell lymphoma has improved over recent years. However, the optimal treatment strategy including the role of radiotherapy remains unknown. We retrospectively analyzed the clinical outcomes of 345 patients with newly diagnosed primary mediastinal large B-cell lymphoma in Japan. With a median follow up of 48 months, the overall survival at four years for patients treated with R-CHOP (n=187), CHOP (n=44), DA-EPOCH-R (n=9), 2nd- or 3rd-generation regimens, and chemotherapy followed by autologous stem cell transplantation were 90%, 67%, 100%, 91% and 92%, respectively. Focusing on patients treated with R-CHOP, a higher International Prognostic Index score and the presence of pleural or pericardial effusion were identified as adverse prognostic factors for overall survival in patients treated with R-CHOP without consolidative radiotherapy (IPI: hazard ratio 4.23, 95% confidence interval 1.48–12.13, P=0.007; effusion: hazard ratio 4.93, 95% confidence interval 1.37–17.69, P=0.015). Combined with the International Prognostic Index score and the presence of pleural or pericardial effusion for the stratification of patients treated with R-CHOP without radiotherapy, patients with lower International Prognostic Index score and the absence of effusion comprised approximately one-half of these patients and could be identified as curable patients (95% overall survival at 4 years). The DA-EPOCH-R regimen might overcome the effect of these adverse prognostic factors. Our simple indicators of International Prognostic Index score and the presence of pleural or pericardial effusion could stratify patients with primary mediastinal large B-cell lymphoma and help guide selection of treatment. 相似文献
9.
Shintaro Akiyama Koji Izutsu Yasunori Ota Tsunao Imamura Osamu Ogawa Atsushi Wake Kazuo Takeuchi 《Medicine》2014,93(26)
A 46-year-old woman underwent upper endoscopy for evaluation of anemia, which revealed whitish granules at the duodenal papilla, diagnosed as duodenal follicular lymphoma (DFL) by biopsy. Computed tomography and abdominal ultrasonography revealed that follicular lymphoma was confined to the duodenum. Seven years after the diagnosis, fluorine-18 fluorodeoxyglucose positron emission tomography scanning revealed multiple lesions including in bone marrow and lymph nodes. Bone marrow biopsy of the right iliac bone revealed diffuse large B-cell lymphoma, indicating systemic dissemination and histologic transformation of the DFL. The patient responded to chemotherapy and has been progression-free for 2.5 years. Although DFL is usually indolent even without any treatment, systemic dissemination with histologic transformation can occur. This case suggests that the life-time follow-up that is usually done for patients with nodal follicular lymphoma should be provided to patients with DFL. 相似文献
10.
Ahmed Ali Elsayed Naoko Asano Koichi Ohshima Koji Izutsu Tomohiro Kinoshita Shigeo Nakamura 《Pathology international》2014,64(7):336-345
To investigate the clinicopathological significance of CD20 expression and Epstein‐Barr virus (EBV) association in Hodgkin and Reed–Sterberg cells of classical Hodgkin lymphoma (CHL), CD20 expression and EBV positivity (by EBER in situ hybridization) were investigated in 389 CHL patients in Japan. They included 74 CD20‐positive cases (19%) and 315 CD20‐negative cases (81%). CD20‐positive cases showed significantly older age at onset (P = 0.018) and higher association with EBV (P = 0.002). Multivariate analysis identified EBV‐positivity (but not CD20‐positivity), presence of B symptoms, thrombocytopenia, elevated serum lactate dehydrogenase and performance status >1 as poor prognostic factors for overall survival (OS). We constructed a new prognostic model with these five factors classifying patients into three groups: low risk, 0–1 adverse factor; intermediate risk, 2–3 factors; high risk, 4–5 factors. This prognostic model could stratify the prognosis of CHL patients (P < 0.0001). For 144 patients (58%) classified into the low‐risk group, the 5‐year OS was 91%. For 92 patients (37%) in the intermediate group, the 5‐year OS was 66%; for 11 patients (5%) in the high‐risk group, the 5‐year OS was 36%. In conclusion, EBV is identified as an independent poor prognostic factor for CHL patients. Therefore, examination of EBV association in CHL is recommended as routine pathologic practice especially in countries where EBV infection prevails. 相似文献