全文获取类型
收费全文 | 542篇 |
免费 | 43篇 |
国内免费 | 2篇 |
专业分类
儿科学 | 17篇 |
妇产科学 | 11篇 |
基础医学 | 100篇 |
口腔科学 | 7篇 |
临床医学 | 48篇 |
内科学 | 117篇 |
皮肤病学 | 10篇 |
神经病学 | 56篇 |
特种医学 | 32篇 |
外科学 | 69篇 |
综合类 | 2篇 |
预防医学 | 53篇 |
眼科学 | 4篇 |
药学 | 30篇 |
中国医学 | 1篇 |
肿瘤学 | 30篇 |
出版年
2022年 | 2篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 18篇 |
2017年 | 13篇 |
2016年 | 11篇 |
2015年 | 12篇 |
2014年 | 17篇 |
2013年 | 31篇 |
2012年 | 30篇 |
2011年 | 52篇 |
2010年 | 11篇 |
2009年 | 7篇 |
2008年 | 35篇 |
2007年 | 39篇 |
2006年 | 44篇 |
2005年 | 53篇 |
2004年 | 36篇 |
2003年 | 52篇 |
2002年 | 29篇 |
2001年 | 7篇 |
2000年 | 8篇 |
1999年 | 8篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1981年 | 4篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1970年 | 2篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1961年 | 1篇 |
1939年 | 1篇 |
1936年 | 1篇 |
1933年 | 1篇 |
排序方式: 共有587条查询结果,搜索用时 0 毫秒
1.
Andersson M Rönnmark J Areström I Nygren PA Ahlborg N 《Journal of immunological methods》2003,283(1-2):225-234
Measurement of human serum molecules with two-site ELISA can be biased by the presence of human heterophilic anti-animal immunoglobulin antibodies (HAIA) that cause false-positive signals by cross-linking the monoclonal (mAb) and/or polyclonal antibodies (pAb) used for the pre- (capture) and post-analyte steps (detection). To evaluate a novel ELISA format designed to avoid interference by HAIA, a target-specific non-immunoglobulin (Ig) affinity protein (affibody) was used to replace one of the antibodies. First, a human IgA-binding affibody (Z(IgA)) selected by phage display technology from a combinatorial library of a single Staphylococcus aureus protein A domain was used. The detection range of IgA standard using an ELISA based on Z(IgA) for capture and goat pAb against IgA (pAb(IgA)) for detection was comparable with that of using pAb(IgA) for both capture and detection. Secondly, another affibody (Z(Apo)) was combined with mAb and used to detect recombinant human apolipoprotein A-1. The affibody/antibody ELISAs were also used to quantify human serum levels of IgA and apolipoprotein A1. To verify that human serum did not cause false-positive signals in the affibody/antibody ELISA format, the ability of human serum to cross-link affibodies, mAb (mouse or rat) and/or pAb (goat) displaying non-matched specificities was assessed; affibodies and antibodies were not cross-linked whereas all combinations of mAb and/or pAb were cross-linked. The combination of affibodies and antibodies for analysis of human serum molecules represents a novel two-site ELISA format which precludes false-positive signals caused by HAIA. 相似文献
2.
Seyrantepe V Poupetova H Froissart R Zabot MT Maire I Pshezhetsky AV 《Human mutation》2003,22(5):343-352
Lysosomal sialidase (EC 3.2.1.18) has a dual physiological function; it participates in intralysosomal catabolism of sialylated glycoconjugates and is involved in cellular immune response. Mutations in the sialidase gene NEU1, located on chromosome 6p21.3, result in autosomal recessive disorder, sialidosis, which is characterized by the progressive lysosomal storage of sialylated glycopeptides and oligosaccharides. Sialidosis type I is a milder, late-onset, normosomatic form of the disorder. Type I patients develop visual defects, myoclonus syndrome, cherry-red macular spots, ataxia, hyperreflexia, and seizures. The severe early-onset form, sialidosis type II, is also associated with dysostosis multiplex, Hurler-like phenotype, mental retardation, and hepatosplenomegaly. We summarize information on the 34 unique mutations determined so far in the sialidase gene, including four novel missense and one novel nonsense mutations found in two Czech and two French sialidosis patients. The analysis of sialidase mutations in sialidosis revealed considerable molecular heterogeneity, reflecting the diversity of clinical phenotypes that make molecular diagnosis difficult. The majority of sialidosis patients have had missense mutations, many of which have been expressed; their effects on activity, stability, intracellular localization, and supramolecular organization of sialidase were studied. A structural model of sialidase allowed us to localize mutations in the sialidase molecule and to predict their impact on the tertiary structure and biochemical properties of the enzyme. 相似文献
3.
A non-parametric bootstrap approach for analysing the statistical properties of SPECT and PET images
Buvat I 《Physics in medicine and biology》2002,47(10):1761-1775
Knowledge of the statistical properties of reconstructed single photon emission computed tomography (SPECT) and positron emission tomography (PET) images would be helpful for optimizing acquisition and image processing protocols. We describe a non-parametric bootstrap approach to accurately estimate the statistical properties of SPECT or PET images whatever the noise properties in the projections and the reconstruction algorithm. Using analytical simulations and real PET data, this method is shown to accurately predict the statistical properties, including the variance and covariance, of reconstructed pixel values for both linear (filtered backprojection) and non-linear (ordered subset expectation maximization) reconstruction algorithms. 相似文献
4.
Iréne?LundEmail author Thomas?Lundeberg Louise?Sandberg Cecilia?Norrbrink?Budh Jan?Kowalski Elisabeth?Svensson 《BMC medical research methodology》2005,5(1):31
Background:
Rating scales like the visual analogue scale, VAS, and the verbal rating scale, VRS, are often used for pain assessments both in clinical work and in research, despite the lack of a gold standard. Interchangeability of recorded pain intensity captured in the two scales has been discussed earlier, but not in conjunction with taking the influence of pain etiology into consideration. 相似文献5.
Gerson M. Struik MSc MD PhD Bram Schermers MSc Ingeborg Mares MSc MD PhD Harold E. Lont MSc MD Jennifer W. Bradshaw MSc MD Bernard ten Haken MSc Ir PhD Theo J. M. Ruers MSc MD PhD Jurgen E. M. Mourik PhD Erwin Birnie MSc PhD Taco M. A. L. Klem MSc MD PhD 《The breast journal》2021,27(8):638-650
Wire-guided localization (WGL) is the standard of care in the surgical treatment of nonpalpable breast tumors. In this study, we compare the use of a new magnetic marker localization (MaMaLoc) technique to WGL in the treatment of early-stage breast cancer patients. Open-label, single-center, randomized controlled trial comparing MaMaLoc (intervention) to WGL (control) in women with early-stage breast cancer. Primary outcome was surgical usability measured using the System Usability Scale (SUS, 0–100 score). Secondary outcomes were patient reported, clinical, and pathological outcomes such as retrieval rate, operative time, resected specimen weight, margin status, and reoperation rate. Thirty-two patients were analyzed in the MaMaLoc group and 35 in the WGL group. Patient and tumor characteristics were comparable between groups. No in situ complications occurred. Retrieval rate was 100% in both groups. Surgical usability was higher for MaMaLoc: 70.2 ± 8.9 vs. 58.1 ± 9.1, p < 0.001. Patients reported higher overall satisfaction with MaMaLoc (median score 5/5) versus WGL (score 4/5), p < 0.001. The use of magnetic marker localization (MaMaLoc) for early-stage breast cancer is effective and has higher surgical usability than standard WGL. 相似文献
6.
Lucie Matrat MD Mathias Ruiz MD Emmanuelle Ecochard-Dugelay MD Irène Loras-Duclaux MD Stéphanie Marotte MD Sophie Heissat MD Pierre Poinsot MD Anne-Laure Sellier-Leclerc MD Justine Bacchetta MD PhD Laurence Dubourg MD PhD Noël Peretti MD PhD 《JPEN. Journal of parenteral and enteral nutrition》2022,46(1):180-189
7.
J. Th. M. Groenewegen Ir. P. J. J. De Meijer 《Pharmacy World & Science》1980,2(1):965-972
Samenvatting Een systeem voor dunnelaagchromatografie wordt beschreven met behulp waarvan twaalf verschillende benzodiazepinen kunnen worden geïdentificeerd en waarmee tevens eventuele ontledingsprodukten kunnen worden aangetoond. Er wordt gebruik gemaakt vanhptlc Silica Gel 60 F254 plaatjes en als loopvloeistof wordt tolueen-methanol (96 + 6) gebruikt.De vlekjes kunnen direct onderuv-licht worden waargenomen; ze kunnen nader worden geïdentificeerd na bespuiten met zwavelzuur en/of het reagens van Bratton Marshall. Van enkele benzodiazepinen kan de identiteit echter pas worden vastgesteld nadat ze zijn gehydrolyseerd. 相似文献
8.
OBJECTIVE: to describe Swedish midwives' reflections on their experiences of caring for teenage girls during pregnancy and childbirth. DESIGN: a hermeneutical approach was used, with focus-group discussions as the method of data collection. SETTING: three focus-group discussions were conducted in a county comprising urban, suburban and rural districts in south-western Sweden. PARTICIPANTS: a voluntary sample of 24 midwives, aged 32-61 years, who were caring for women in different birth settings, participated in 2004. FINDINGS: two main themes, with three sub-themes each, were generated by the midwives: (1) the teenage mother, as 'teenagers who are proud of becoming mothers', 'teenagers who are unprepared for becoming mothers' and 'teenagers with an immigrant background', and (2) 'the midwives' wish to care for the teenage mother', as 'taking the teenage mother seriously', 'being an important person for the teenage mother', and 'being a help for the teenage mother'. KEY CONCLUSIONS AND IMPLICATIONS FOR PRACTICE: the findings provide some understanding of the unique characteristics of caring for teenage mothers. For the midwives, the most important aspects of caring for the teenage mother included taking the teenage mother seriously, allowing the midwife herself to become an important person for the teenage mother, and being a help to the mother. This help requires the midwife to balance the different needs of each teenage mother. 相似文献
9.
Gabriela Flores Por Ir Chean R. Men Owen O’Donnell Eddy van Doorslaer 《Journal of health economics》2013
Public providers have no financial incentive to respect their legal obligation to exempt the poor from user fees. Health Equity Funds (HEFs) aim to make exemptions effective by giving NGOs responsibility for assessing eligibility and compensating providers for lost revenue. We use the geographic spread of HEFs over time in Cambodia to identify their impact on out-of-pocket (OOP) payments. Among households with some OOP payment, HEFs reduce the amount paid by 35%, on average. The effect is larger for households that are poorer and mainly use public health care. Reimbursement of providers through a government operated scheme also reduces household OOP payments but the effect is not as well targeted on the poor. Both compensation models raise household non-medical consumption but have no impact on health-related debt. HEFs reduce the probability of primarily seeking care in the private sector. 相似文献
10.
Camilla Rubenson Eva Svensson Iréne Linddahl 《Scandinavian journal of occupational therapy》2013,20(4):205-214
The purpose of this qualitative study was to explore experiences of returning to work after rehabilitation, from the viewpoint of people with acquired brain injury. A purposive sampling of eight informants was made, and data were collected through personal interviews with open questions. The data were then analysed through qualitative content analyses in five steps, resulting in four main categories of experiences: “A new person”, “Stimulants and fellowship”, “Understanding and support”, and “To reach insight” with appurtenant sub-categories. The overarching theme was “Returning to work after acquired brain injury is a long process”. The conclusion of this study is that returning to work after acquired brain injury requires motivated individuals, flexible work, accommodating labour management, and prolonged environmental support. This study also shows that the informants need support for a long period of time to reach a balance and to obtain a functional working role. In order to achieve this, client-centred rehabilitation, professional teamwork, and longer follow-up periods than those of today are required. 相似文献