全文获取类型
收费全文 | 1196篇 |
免费 | 85篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 72篇 |
妇产科学 | 11篇 |
基础医学 | 174篇 |
口腔科学 | 31篇 |
临床医学 | 112篇 |
内科学 | 275篇 |
皮肤病学 | 21篇 |
神经病学 | 23篇 |
特种医学 | 243篇 |
外科学 | 77篇 |
综合类 | 60篇 |
预防医学 | 60篇 |
眼科学 | 22篇 |
药学 | 45篇 |
中国医学 | 9篇 |
肿瘤学 | 44篇 |
出版年
2021年 | 4篇 |
2020年 | 6篇 |
2019年 | 11篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 7篇 |
2015年 | 19篇 |
2014年 | 39篇 |
2013年 | 63篇 |
2012年 | 29篇 |
2011年 | 31篇 |
2010年 | 39篇 |
2009年 | 61篇 |
2008年 | 33篇 |
2007年 | 49篇 |
2006年 | 28篇 |
2005年 | 27篇 |
2004年 | 21篇 |
2003年 | 16篇 |
2002年 | 22篇 |
2001年 | 16篇 |
2000年 | 15篇 |
1999年 | 16篇 |
1998年 | 78篇 |
1997年 | 53篇 |
1996年 | 63篇 |
1995年 | 45篇 |
1994年 | 49篇 |
1993年 | 44篇 |
1992年 | 13篇 |
1991年 | 9篇 |
1990年 | 12篇 |
1989年 | 41篇 |
1988年 | 30篇 |
1987年 | 27篇 |
1986年 | 23篇 |
1985年 | 16篇 |
1984年 | 22篇 |
1983年 | 17篇 |
1982年 | 17篇 |
1981年 | 26篇 |
1980年 | 17篇 |
1979年 | 16篇 |
1978年 | 18篇 |
1977年 | 23篇 |
1976年 | 9篇 |
1975年 | 14篇 |
1974年 | 8篇 |
1972年 | 6篇 |
1971年 | 7篇 |
排序方式: 共有1290条查询结果,搜索用时 9 毫秒
1.
W Kepron C J Jackson A H Sehon 《International archives of allergy and applied immunology》1987,82(3-4):468-470
Newborn mongrel dogs were sensitized with conjugates of ovalbumin (OA) and 2,4-dinitrophenol (OA-DNP3) in the presence of Al(OH)3 to produce high levels of anti-OA and anti-DNP IgE antibody. At 4-6 months of age, when anti-DNP and anti-OA antibody levels reached titers of 64 by passive cutaneous anaphylaxis, the dogs underwent separate inhalation and intravenous challenges with conjugates of DNP and bovine gamma globulin (DNP15-BGG) and OA. Inhalation challenge with DNP15-BGG and OA resulted in 5- and 10-fold increases in airflow resistance, respectively. Intravenous challenge with either DNP15-BGG or OA produced profound anaphylaxis with 60-80% decreases in blood pressure, cardiac output and regional blood flows in the carotid, superior mesenteric and renal arteries, and the distal aorta. Treatment of sensitized dogs with 5 doses of 20 mg of conjugates of DNP and polyvinyl alcohol (DNP2-PVA) on alternate days resulted in suppression of anti-DNP IgE antibody production; abrogation of established airway and vascular anaphylactic sensitivities; no change in regional blood flows, and no effect on sensitivities to challenge with OA. 相似文献
2.
Retrorenal colon: implications for percutaneous diskectomy 总被引:1,自引:0,他引:1
It has been recommended that computed tomography (CT) with the patient prone be performed in every patient undergoing percutaneous diskectomy; this would enable detection of a retrorenal location of the colon, which could interfere with the percutaneous procedure. In this evaluation of 346 prone CT studies, only one patient (0.29%) was found to have retrorenal or retropsoas bowel that would have been perforated at diskectomy. Because of this extremely low prevalence, the performance of prone CT in every patient undergoing percutaneous lumbar diskectomy is not believed to be necessary. 相似文献
3.
The ouabain-resistant mutant cell lines, HOA-1 and HOA-20 were developed from WI-L2-729-HF2 by cloning with increasing concentration of ouabain. Both parent and mutant cell lines were resistant to base analogues, 6-thioguanine (6-TG) and 8-azaguanine (8-AG) to the level of 20 micrograms/ml in the culture medium. The parent cell line WI-L2-729-HF2 was highly sensitive to ouabain, whereas HOA-1 and HOA-20 were resistant to ouabain to the level of 1 microM and 20 microM, respectively. However, all the cell lines were sensitive to HAT-selective medium which is essential for hybrid selection after fusion. All three lymphoblastoid cell lines were positive for Epstein-Barr virus nuclear antigen (EBNA), secreted TNF-beta (lymphotoxin) without any external stimulation, secreted trace amounts of IgG(kappa), which was also present in their cytoplasm and had IgM(kappa) as surface bound immunoglobulin. They also expressed the CD20, CD71 (transferrin receptor) as surface antigens. In addition to these antigens, HOA-20 also expressed CD38 antigen. The karyotype analysis of these cell lines revealed modal chromosomal numbers ranging from 40 to 47. The HLA-A, -B and -C antigens expressed by WI-L2-729-HF2 and its mutants HOA-1 and HOA-20 were identical. Both the HOA-1 and HOA-20 mutants were found suitable for the generation of hybrids after fusion with EBV-transformed human B-lymphocytes. 相似文献
4.
5.
6.
郭永建 《福建医科大学学报》1994,(1)
采用间接ELISA检测23名肾移植受者血清巨细胞病毒(CMV)抗体,共检出18名(78%)活动性CMV感染,其中10名(44%)为原发性感染。结果证实CMV-IgE和-IgA具有较好的血清学诊断价值,优于CMV-IgM。 相似文献
7.
CA von Arnim R Spoelgen ID Peltan M Deng S Courchesne M Koker T Matsui H Kowa SF Lichtenthaler MC Irizarry BT Hyman 《The Journal of neuroscience》2006,26(39):9913-9922
The beta-amyloid (Abeta) precursor protein (APP) is cleaved sequentially by beta-site of APP-cleaving enzyme (BACE) and gamma-secretase to release the Abeta peptides that accumulate in plaques in Alzheimer's disease (AD). GGA1, a member of the Golgi-localized gamma-ear-containing ARF-binding (GGA) protein family, interacts with BACE and influences its subcellular distribution. We now report that overexpression of GGA1 in cells increased the APP C-terminal fragment resulting from beta-cleavage but surprisingly reduced Abeta. GGA1 confined APP to the Golgi, in which fluorescence resonance energy transfer analyses suggest that the proteins come into close proximity. GGA1 blunted only APP but not notch intracellular domain release. These results suggest that GGA1 prevented APP beta-cleavage products from becoming substrates for gamma-secretase. Direct binding of GGA1 to BACE was not required for these effects, but the integrity of the GAT (GGA1 and TOM) domain of GGA1 was. GGA1 may act as a specific spatial switch influencing APP trafficking and processing, so that APP-GGA1 interactions may have pathophysiological relevance in AD. 相似文献
8.
CA Schroeter† L Kaas† JJ Waterval† PM Bos‡ HAM Neumann§ 《Journal of the European Academy of Dermatology and Venereology》2007,21(9):1170-1174
AIM: The aim of this pilot study was an investigation on photodynamic therapy (PDT) whether it is a good alternative for treating periungual and subungual warts of the hands. STUDY DESIGN: Twenty patients (mean age: 30.5 years) with a total of 40 periungual and subungual warts were treated with PDT. A photosensitizer, 20%delta-aminolevulinic acid was applied on the warts. After a mean incubation time of 4.6 h (SD: 1.2), the warts were irradiated with the VersaLight for 5-30 min (15.2 +/- 4.3 min). RESULTS: After a mean of 4.5 treatments a mean clearance of 100% was achieved in 90% of the patients. One patient (5%) showed a clearance of 50% and another showed no improvement. The subungual or periungual location of the wart had no influence on the number of treatments or end result (P > 0.05). There were two recurrences during the mean follow-up period of 5.9 months (SD: 7.6). Besides mainly pain and hyperpigmentation, most treatments had no side-effects. CONCLUSION: PDT can offer a good alternative for treating periungual warts of the hands. Larger studies are indicated. 相似文献
9.