Predictors for Survival and Distribution of 21-Gene Recurrence Score in Patients With Pure Mucinous Breast Cancer: A SEER Population-Based Retrospective Analysis

Background

Pure mucinous breast cancer (PMBC) is a rare pathologic type of breast cancer, the prognostic factors of which have not been clearly defined. This study aimed to analyze the prognostic markers and distribution of 21-gene recurrence score (RS) in patients with PMBC.

直缘乌头内生真菌Alternaria solani化学成分研究

摘 要 目的：对直缘乌头内生真菌Alternaria solani 的化学成分进行研究。方法: 采用各种柱色谱对直缘乌头内生真菌Alternaria solani的甲醇提取物进行分离纯化,经波谱数据 (¹ H-NMR,¹³ C-NMR,MS) 进行结构鉴定。结果:分离得到15个化合物,分别鉴定为：20 羟基麦角甾 4,6,8(14),22-四烯-3-酮（1）、(22E,24R)- 麦角甾-4,6,8(14),22-四烯-3-酮（2）、(22E,24R)-麦角甾-7,22-二烯-3-β-醇（3）、24-亚甲基-麦角甾-7-烯-3β-醇（4）、过氧麦角甾醇（5）、cyclo(D)-Pro-(D)-Leu（6）、cyclo-(L-Val-L-Pro)（7）、cyclo-(S-Pro-S-Ile)（8）、tentoxin（9）、porrotoxin（10）、7-dehydroxyl-zinniol（11）、zinniol（12）、8-zinniol methyl ether（13）、3-甲氧基-4-甲基-5-(3’,3′-二甲基烯丙氧基)-2-甲氧基甲基苯甲酸（14）、拟盘多毛孢H2-倍半萜内酯（15）。结论:从直缘乌头内生真菌Alternaria solani中分离得到15个化合物,其中化合物2~10,12~15是首次从该植物内生真菌中分离得到。     

Delivery of radix ophiopogonis polysaccharide via sucrose acetate isobutyrate-based in situ forming systems alone or combined with its mono-PEGylation

This work aimed to achieve long-lasting delivery of radix ophiopogonis polysaccharide (ROP) by sucrose acetate isobutyrate (SAIB)-based in situ forming systems (ISFSs) alone or combined with mono-PEGylation of ROP. When the ‘90%SAIB/10% solvent’ system was used, the mean residence time (MRT) of ROP was prolonged by 4.3 5?～?7.00 times and the initial release rate was reduced significantly. However, this system was only suitable for days-long sustained release of ROP in short-term therapy. As to the ‘SAIB/additives/solvent’ system containing mono-PEGylated ROP, the results indicated that SAIB/poly(d,l-lactide-co-glycolide) (PLGA)/N-methyl-2-pyrrolidone (NMP) was superior to SAIB/polylactic acid (PLA)/NMP and SAIB/PLA/ethanol in controlled release. Moreover, weeks- to months-long (16–60 d) smooth release of ROP could be achieved by varying the concentration (10–30%) and molecular weight (MW) of PLGA (10–50?kDa) or by employing a moderate MW of PEGylated ROP (～20 or ～30?kDa). With further increasing the conjugate MW to ～40?kDa, the contribution of drug elimination to its plasma retention seemed to surpass that of the SAIB-based system, resulting in that the system no longer had an obvious influence on the in vivo behavior of the conjugate. Besides, the results of host response confirmed that with less solvent being used, the SAIB-based systems showed a higher biocompatibility than the PLGA-based systems, suggesting that they could be freely chosen in the prevention and/or cure of chronic diseases.     

外周血中hnRNP A2/B1含量及其在肺癌诊断中的意义

目的用FQ-RT-PCR技术检测外周血中hnRNPA2/B1表达水平及在肺癌诊断中的意义.方法用FQ-RT-PCR技术定量分别检测18例健康体检者、12例肺部良性疾病和30例肺癌患者外周血中hnRNPA2/B1 mRNA,并用β-actin对hnRNPA2/B1表达水平进行标化.结果 hnRNPA2/B1与β-actin的比值在正常对照组和良性肺部疾病组间差异无显著性(P>0.05),而肺癌组均高于前两组(P<0.05);hnRNPA2/B1基因在低分化鳞癌中的表达水平很低,与其它类型肺癌差异有显著性(P<0.05).结论 hnRNPA2/B1在血中表达水平的差异在肺癌诊断中具有一定临床应用价值,并与肺癌的病理分级有一定相关性.     

Micronemal protein 13 contributes to the optimal growth of Toxoplasma gondii under stress conditions

Parasitology Research - Toxoplasma gondii is a ubiquitous parasitic protozoan infecting humans and a wide variety of animals. Fast-replicating tachyzoites during acute infection and slowly growing...     

Investigating social inequalities in older adults’ dentition and the role of dental service use in 14 European countries

Background

Oral disease, despite being largely preventable, remains the most common chronic disease worldwide and has a significant negative impact on quality of life, particularly among older adults.

Objective

This study is the first to comprehensively and at a large scale (14 European countries) measure the social inequalities in the number of natural teeth (an informative oral health marker) in the over 50-year-old population and to investigate the extent to which such inequalities are attributable to dental service use.

Methods

Using Wave 5 of the Survey of Health, Ageing and Retirement in Europe, which included internationally harmonized information on over 50,000 individuals across 14 European countries, we calculated Gini and Concentration indices (CI) as well as the decompositions of CIs by socioeconomic factors.

Results

Sweden consistently performed the best with the lowest inequalities as measured by Gini (0.1078), CI by income (0.0392), CI by education (0.0407), and CI by wealth (0.0296). No country performed the worst in all inequality measures. However, unexpectedly, some wealthier countries (e.g., the Netherlands and Denmark) had higher degrees of inequalities than less-wealthy countries (e.g., Estonia and Slovenia). Decomposition analysis showed that income, education, and wealth contributed substantially to the inequalities, and dental service use was an important contributor even after controlling for income and wealth.

Conclusions

The study highlighted the importance of comprehensively investigating oral health inequalities. The results are informative to policymakers to derive country-specific health policy recommendations to reduce oral health inequalities in the older population and also have implications for oral health improvement of the future generations.

     

Assessment of the Remediation Effect of Nano-hydroxyapatite in Exogenous Pb-contaminated Soil Using Toxicity Characteristic Leaching Procedure and Soil Enzyme Activities

This study assessed the level of Pb in soil and five wild plant species (Calotropis procera, Datura alba, Parthenium hysterophorus, Cenchrus ciliaris and Ricinus communis) during all the four seasons. Two busy roads varying in age and traffic volume were selected i.e., Faisalabad–Sargodha road (FSR) and Pindi Bhattian to Lillah (M-2) in the Punjab, Pakistan. Results showed raised levels of Pb in both plants and soil samples along both roads. The range of Pb concentration in plants was 0.08–3.98 and 1.95–4.74 mg kg^??1 for soil. Higher Pb contamination was recorded along FSR road as compared to M-2. Among seasons, the higher Pb concentration was found during summer, probably due to very high temperature. Among all the plants studied, Calotropis procera accumulated the highest level (3.98 mg kg^??1 dry wt.) of Pb; Thus, it can be used as good biomonitor/phytoremediator at Pb contaminated areas.     

针刺结合牵引治疗腰椎间盘突出症的疗效观察

目的探讨针刺结合牵引治疗腰椎间盘突出症的疗效。方法研究对象为240例腰椎间盘突出症患者,随机分为治疗组和对照组各120例。治疗组采用针刺结合牵引治疗,对照组采用单独牵引治疗,观察和比较两组症状及体征改变情况。结果治疗组临床治愈92例,治愈率76.7%;对照组临床治愈70例,治愈率58.3%。经统计学处理,χ2=10.69,P0.01,两组有显著性差异。结论针刺结合牵引治疗腰椎间盘突出症的疗效明显优于单独牵引治疗。