Determination of subpopulations of T- and B-lymphocytes in human blood using an immunofluorescence method

The immunofluorescent technique for assaying T- and B-lymphocyte subpopulations, using monoclonal (OCT3, OCT4, OCT8) and monospecific antibodies is described. The results of immunocompetent cell determinations in normal subjects and in patients are presented. A conclusion is made that use of universal immunofluorescent method for assays of the lymphocyte subpopulations widens the possibilities of diagnosis of immunodeficiency states.     

The late examination results in children with pylorospasm and dyskinesia of the proximal sections of the small intestine]

The article analyses the late-term results of examination of 46 children with neurogenous dyskinesias of the gastrointestinal tract which were recognised in infancy for the first time. The author believes that the functional, motor-evacuation, and sphincter disorders of the proximal parts of the digestive tube lead to the development of organic noninfectious diseases of the gastroduodenal system.     

Synthesis and antihypoxic activity of 1,2,4-triazoles, 1,3-dithiane-1,1,3,3-tetraoxides,and 1,4-benzothiazin-3(4H)-one

Translated from Khimiko-farmatsevticheskii Zhurnal, Vol. 26, No. 2, pp. 38–41, February, 1992.     

Treatment of strictures of the urethra in men

Treatment of urethral strictures was conducted in 84 male patients, aged 15 to 78 years. Combined treatment included optical (retrograde and antegrade) urethrotomy and transurethral electroresection (TUR) of scar tissues, combined with postoperative endourethral phonophoresis with lidase (64 units) and an antibiotic. Retrograde urethrotomy was performed under visual control. Scar tissues were dissected layer by layer until the resectoscope could be introduced. Antegrade urethrotomy was performed after bougies were passed through the stricture. To improve the results of endourethral surgery, all patients underwent endourethral lidase and antibiotic phonophoresis, using "C" irradiator of the Soviet Sterzhen' device, on postoperative day 3. The duration of phonophoretic session was 6 to 12 min at 0.1-0.3 kW. There were 8 to 10 sessions to a course of physiotherapy. This combined treatment stabilized urodynamic parameters at 1 to 1.5 years' follow-up, as evidenced by significantly increased mean and peak urinary flow rate and shorter bladder evacuation time. There were basically no postoperative relapses in 92.1% of the patients.     

Antibodies to structural and nonstructural gag-coded proteins of type-D retroviruses in humans with lymphadenopathy and AIDS

The sera of patients with lymphadenopathies (children, suffering from primary immune deficiencies) and AIDS were analysed for the presence of antibodies against D-type retroviruses by means of radioimmunoprecipitation (RIP) and Western blotting. It was established that these sera contain the antibodies derived against p27, the major structural protein of type-D retroviruses, in a considerable percentage (12/80 for lymphadenopathy (LAP) patients and 9/12 for AIDS patients). Some serum samples reacted in RIP with Pr78gag, the precursor of gag-coded proteins of these viruses. The results obtained for AIDS patients are in contrast with those reported by other research groups. Nevertheless, it seems highly probable that D-type retroviruses are truly associated with human immunodeficiencies, as has been shown for simian ones.