流行性乙型脑炎特异性IgM(ELISA)诊断试剂盒的改进和应用

流行性乙型脑炎夏秋季发病 ,与中毒性痢疾等其他脑部疾患易相混 ,仅靠临床症状诊断 ,可靠性差 ,易延误治疗 ,造成后患。因此 ,我们改进了乙型脑炎特异性IgM诊断试剂盒 ,缩短时间 ,当天抽血 10 0min内即可出报告 ,在短时间内 ,为临床提供可靠依据。     

升主动脉成形术中期效果分析

Objective Reduction ascending aortoplasty is an alternative procdure to the replacement of the ascending aorts in case of ascending aorta dilation. However,its applicabikity is still under debate.This retrospective study was designed to evaluuate the midterm follow-up of unsupported ascending reduction aortoplasty for of the ascending aorta in petients with aortic valve dis- ease.Methods From October ,1996 to April,2007, a total of 54 patients with aortic valve disease and dilatation of the ascending aorta underwent unsupported reduction aortoplasty in combination with aortic valve replacement at our institution The diameter of the ascending aorta was measyred before and early after sugery and then later between 13 and 96 menths [mean (23 ±16)months] posto- perativeiy using echocardiography.Results Two patients were dead with thean overall perioperative mortality rate was of 3.7%. The reduction aortoplasty decreased the diameter of the aorta from (45.77±6.02) mm p～eope～afive]y to (34.67 ～4.81) mm early after surgery (P<0.01). During follow-up, the diameter d aorta increased from (34.67±4.81) mm early after surgery to (37.65± 6.35) mm after a mean follow-up of (23±16) months (P<0.01), including the diameters are greater than > 45 mm within 5 pa- tients. Aortic stenosis and an early postoperative diameter greater than 40 mm m'e independent risk factors for redilatation. Conchusion Because of the unsatisfied midterm follow-up redilation of unsupported reduction aortoplasty for dilation of the ascending aorta with aortic valve disease, this group of patients needs continued intimate fallow-up or even reoperation. The patients of stenosis is the surtable indication for RAA, and it is necessary to reduce the diameter of aorta to be lees than 40 mm to prevent redilation.     

带状疱疹早期诊断的体会

带状疱疹为常见病、多发病,典型病例不难诊断,但在疾病早期或临床表现不典型常可导致误诊。1991～1992年,我们诊治7例带状疱疹患者,早期均被误诊。现报道如下。 1 临床资料 年龄 性别 主要表现 初诊诊断 出疹部位 34 男 发热,乏力,感冒,病毒性 右肋部纳差, 右肋部 肝炎 疼痛 69 男 头痛,全身乏力 脑血管痉挛,右侧额顶部 脑动脉硬化     

灵龟八法按时开穴针刺对豚鼠SOD及MDA影响的实验研究

[目的]通过观察灵龟八法按时开穴针刺对豚鼠SOD及MDA影响,从自由基角度来探讨灵龟八法抗衰老的机理。[方法]以健康豚鼠为观察对象,以超氧化物歧化酶(SOD)和丙二醛(MDA)为观测指标,在子、卯、午、酉四个时辰里按照灵龟八法开穴针刺,连续针刺7d。[结果]在四个时辰里,按灵龟八法针刺后,除午时外,其它时辰均可提高健康豚鼠SOD活力;除子时外,其它时辰均可降低丙二醛的含量。[结论]在健康生理状态下,灵龟八法按时针刺对SOD和MDA的含量具有调整作用。灵龟八法按时针刺可减轻自由基对机体的损害,从而实现延缓机体衰老的作用。     

脂质体冻结的临界参数

本文研究含亲水性药物脂质体,在冻干/再水化过程中改善质量的途径。冻干包括三个步骤,先是产品冻结,以后开始升华(初级干燥),最后次级干燥。为了在再水化后保持最高的药物量,在三个步骤中均需要最佳的操作参数。作者以亲水而不与双分子层相互作用的羧基荧光素(CF)为模型药物,以氢化大豆磷脂和双十六烷基磷酸酯等材料,用经典薄膜法制备负电荷多层脂质体。并研究冻结速度、温度、时间(样品保持于选定温度的时间)以及不同类型和浓度对冻结保护剂的影响。