脑脊液细胞学检查在神经系统疾病诊疗中的应用价值——附464次检查结果报告

采用侯氏改良的Sayk氏细胞沉淀室进行脑脊液细胞学检查464例次,其中异常326例次(70.3％)。实际病例267例,结合临床支持原诊断的111例(41.6％),有诊断价值82例(30.7％),无诊断价值74例(27.7％),有诊断意义共193例(72.3％)。与传统的计数板法比较具有更大的诊断价值,尤其对中枢神经系统感染、脑膜癌病、脑膜白血病以及出血性脑血管的诊断、鉴别和疗效制定具有独特的临床意义。     

sd-LDL在非乙醇性脂肪性肝病中的应用价值

目的 探讨小而密低密度脂蛋白(sd-LDL)在非乙醇性脂肪性肝病中的应用价值.方法 回顾性纳入2018年4月—2019年5月就诊于复旦大学附属华山医院门诊的非乙醇性脂肪性肝病病人87例,以87例健康体检者作为对照.收集所有研究对象的一般临床资料、血脂水平及FibroTouch肝脏脂肪衰减值.根据肝脏脂肪衰减度将非乙醇性...     

口腔健康教育与局部用氟对青少年固定矫治中釉质脱矿的影响

目的:探讨口腔健康教育与局部用氟对青少年固定矫治后釉质脱矿发生率的影响。方法:将120例青少年矫治患者随机分成3组:第1组为对照组40例(638颗),该组患者不进行任何行为干预;第2组40例(643颗),对患者及家长进行口腔健康教育,但不涂氟;第3组40例(639颗),除了对患者和家长进行口腔健康教育外,同时用氟化物处理牙面。结果:3组患者牙釉质脱矿的比率分别为18.18%、6.53%和3.59%,各组间两两比较均有显著性差异。结论:口腔健康教育与使用氟化物对预防正畸牙釉质脱矿是有效和可行的。     

美诺佳与硫酸镁在治疗输注脂肪乳剂所致静脉炎的疗效比较

目的比较美诺佳涂抹与硫酸镁湿敷在治疗静脉输入脂肪乳剂所致静脉炎的疗效。方法随机将2013年10月~2015年10月40例因静脉输入脂肪乳剂后所致静脉炎患者分为观察组与对照组,每组各20例。观察组采用美诺佳涂抹在静脉炎的血管上方,对照组采用传统的50%硫酸镁湿敷在静脉炎血管上方。观察治疗72 h后,比较两组患者静脉炎的治疗效果。结果观察组静脉炎治疗显效13例,总有效率为100%,对照组静脉炎治疗显效5例,总有效率为65%,观察组的疗效明显优于对照组(P0.05)。结论美诺佳在治疗静脉输入脂肪乳剂所致静脉炎有较好效果,且操作简单,可在临床推广应用。     

学习CCMD－2－R的几点体会

学习ＣＣＭＤ－２－Ｒ的几点体会耿德勤，杨森，荣良群，陆正齐，朱本亮看到新的版本ＣＣＭＤ－２－Ｒ、ＤＳＭ－Ⅳ和ＩＣＤ－１０等书刊，我们青年医生甚为高兴，并组织了学习与讨论。经过国内知名专家修订的ＣＣＭＤ－２－Ｒ的确具有很大的实用意义，对广大神经精神科工...     

丹红注射液治疗急性脑梗死的临床研究

丹红注射液是中药丹参和红花经科学提取的复方制剂。能有效抑制血小板黏附、聚集、激活的作用,并能抗血栓形成、改善微循环等作用调节脑的能量代谢,增加缺血区血流量,缩小梗塞面积,减轻脑水肿,从而改善脑梗死的神经功能缺损症状。笔者利用丹红注射液治疗发病72h内住院的急性脑梗死患者38例进行分析,现报道如下。     

糖尿病足坏疽伴软组织感染的多学科团队协作诊疗一例

糖尿病足溃疡是糖尿病较严重的并发症之一,是目前非创伤性截肢的首要病因。糖尿病足溃疡可由多种因素共同诱导,单一的专科治疗往往效果不佳,需多学科序贯、联合协作治疗,才可避免截肢、脓毒血症甚至死亡的不良预后。本文报道1例糖尿病足溃疡患者的主要诊治经过,于外院行动脉开通后,仍发展至坏疽合并感染;经内分泌科、足踝外科、血管外科、感染科等多学科会诊,共同制订诊疗方案,以促进患者溃疡愈合,有效避免大截肢,减少围手术期并发症发生,提高其生存质量。     

重组腺病毒介导突变型低氧诱导因子1α基因转染脂肪间充质干细胞并促进其增殖

BACKGROUND:To improve the survival rate of transplanted tissue, most scholars focus on cell therapy, particularly cell-assisted fat grafting. OBJECTIVE:To analyze the effect of recombinant adenovirus-mediated hypoxia inducible factor 1alpha (HIF1α) mutant on survival rate of transplanted fat particles through transfection of adipose-derived mesenchymal stem cells. METHODS:Recombinant adenovirus-mediated triple-mutant HIF1α was inserted into an adenovirus pAdEasy-1 system, followed by viral packaging and titer determination. Human adipose-derived mesenchymal stem cells were cultured, passaged and identified, and subsequently transfected with three kinds of viruses and blank vector (experimental group with transfection of the triple mutant of HIF1α; positive control group; negative control group; blank control group). Transfection efficiency was determined using enhanced green fluorescent protein labeling. Additionally, MTT assay was used to detect cell proliferation. RESULTS AND CONCLUSION:The recombinant adenovirus was successfully constructed and packaged in line with transfection requirements. Moreover, adipose-derived mesenchymal stem cells were successfully identified by adipogenic, osteogenic and chondrogenic induction and could be used as seed cells for subsequent experiments. RT-PCR results showed that HIF1α mRNA expression in the experimental group and positive control group was significantly higher than that in the other two groups(P < 0.05). Western blot analysis showed that the relative absorbance value in the experimental group was significantly higher than that in the other three groups (P < 0.05). A significant increase in the cell proliferation was found in the experimental group, significantly different from the other three groups (P < 0.05). Therefore, our findings indicate that transfection of adenovirus-mediated triple-mutant HIF1α not only can sustain the expression of target protein in transfected adipose-derived mesenchymal stem cells under normoxic conditions, but also can promote the proliferation of transfected adipose-derived mesenchymal stem cells.     

急性脑梗死合并上消化道出血临床分析

上消化道出血是急性脑梗死的早期严重并发症之一,可加重脑功能损害,提示预后不良。本文分析我院2009-01—2012-06住院的急性脑梗死合并上消化道出血48例患者的资料,进行临床分析如下。1资料与方法1.1一般资料病例均为我院神经内科住院的急性脑梗死患者320例,合并上消化道出血患者48例。所有病例均符