白藜芦醇诱导急性T淋巴母细胞白血病Jurkat细胞凋亡

目的：探讨白藜芦醇抑制急性T淋巴母细胞白血病Jurkat细胞增殖、引发S期阻滞和诱导凋亡的作用。方法：白藜芦醇体外处理培养的Jurkat细胞后,采用MTT法测定细胞活力,Wrigh-Giemsa染色、Hoechest 33258/PI荧光染色和透射电镜观察Jurkat细胞的形态学改变;流式细胞术分析细胞周期。结果：白藜芦醇0.2 mmol·L^-1处理组的抑制Jurkat细胞增殖率达64.01%,并具有剂量和时间依赖性。白藜芦醇处理细胞24 h后可见凋亡形态学改变,Hoechest 33258/PI染色显示白藜芦醇处理组细胞部分细胞核呈亮蓝色,随培养时间延长,亮蓝色荧光染色细胞数量逐渐增多。Wrigh-Giemsa染色和透射电镜观察发现部分细胞体积缩小、染色质浓缩及边集等现象的出现。流式细胞术检测表明,0.05 mmol·L^-1白黎芦醇处理48 h组62.57％的细胞被阻滞于细胞周期S期,亚二倍体细胞数量12.01%,未加药对照组细胞亚二倍体细胞数量2.05%。结论：白藜芦醇可以抑制Jurkat细胞的增殖,引起细胞周期的S期阻滞,并诱导其发生凋亡     

中国南方11个少数民族血清转铁蛋白C亚型的遗传多态性

用超薄等电聚焦电泳和利凡诺-硫酸亚铁铵样品处理法,分析中国南方11个少数民族共计2121人的血清转铁蛋白C(TfC)亚型多态。结果Tf~(C1),Tf~(C2)和Tf~(Dhi)存在于所有被查群体中,而Tf~B则完全缺乏。在3个群体中发现Tf~(C4),6个群体中发现Tf~(C3),二者的频率都小于0.01。在布依族中发现罕见的TfC4-2型,黎族中发现TfC4-4型,哈尼族中发现TfC4-1型,哈尼族和黎族中分别发现TfC3-2型;另在布依族和彝族中发现TfDChi纯合子。11个少数民族的Tf~(C2)频率变动范围为0.25～0.38,比以前报道的中国汉族群体Tf~(C2)频率为0.18～0.25高得多,特別是白族群体的Tf~(C2)频率已达到世界范围的最高值(0.38)。笔者认为中国南方少数民族Tf~(C2)频率高的原因,除与民族差异有关外,可能还与地理环境气候及人群相对隔离有关。Tf~(C4)虽然在中国人群中出现的频率较低,但似乎支持美印地安人中高频率的Tf~(C4)来源于祖先的蒙古人种这一论点。     

中华民族永生细胞库的建立

目的 为保存中国不同民族基因组,完整建立中国各民族永生细胞库,供永久性研究需要。方法 按照严格的采样标准和“知情同意”原则,采集不同民族群体外周血样,利用EB病毒转化B淋巴细胞为永生细胞的技术,建立中国各民族永生细胞库。结果 已建立了47个民族70个群体（含民族支系）的3982株永生细胞株,并保存了7210份DNA样本。建立了较为成熟和稳定的利用EB病毒转化B淋巴细胞为永生细胞的技术。结论 这是目前规模最大的较为完整的中国国家级中国各民族永生细胞库,可供永久性研究需要。已向国内多家人类基因组相关单位提供了细胞株和 DNA,进行相关研究。     

中国南方11个少数民族血清转铁蛋白C亚型的...

Transferrin subtypes were determined by isoelectric focusing (IEF) and a special treatment of sera with Rivanol- ferrous ammonium sulfate (FAS) in a total of 2121 individuals from 11 southern minority populations in China, including Hani minority (217), Buyi minority (209), Tujia minority (168), Miao minority (197), Zhuang minority (171), Yao minority (210), Bai minority (178), Dong minority (157), Yi minority (206), Qiang minority (219) and Li minority (189). TfC1, TfC2 and TfDChi were present in all the populations, whereas TfB was totally lacking. TfC4 was found in only 3 populations and TfC3 in 6; both were present at frequencies of less than 0.01 in all the populations. We found rare phenotypes TfC4-2, TfC4-4, TfC4-1, TfC3-2 and TfD Ching in these populations. The distributions of TfC2 and TfC4 frequency were notable. In these minority groups, the frequencies of the C2 gene were higher (0.25-0.38) than those of the majority Chinese Han nationality (0.18-0.25). In the Bai minority, the TfC2 gene frequency was as high as 0.38, the highest value found in the world to date. Besides being found in US and South American, the TfC2 allele is also present in both Han and minority nationalities in China, although at a frequency less than 0.01, suggesting that the C4 allele probably to ancestral already existed in the ancestral Mongoloid population at a low frequency prior trial separation and increased in frequency in Amerindians due to genetic drift or selection.