The clinicopathologic spectrum of segmental membranous glomerulopathy

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Resveratrol-loaded PLGA nanoparticles mediated programmed cell death in prostate cancer cells

Resveratrol (RL), a natural polyphenol, is known for its diverse biological effects against various human cancer cell lines. But low aqueous solubility, poor bioavailability, and stability limit its efficacy against prostate cancer. In this study polymeric nanoparticles encapsulating resveratrol (RLPLGA) were designed and their cytotoxic and mode of apoptotic cells death against prostate cancer cell line (LNCaP) was determined. Nanoparticles were prepared by solvent displacement method and characterized for particle size, TEM, entrapment efficiency, DSC and drug release study. RLPLGA exhibited a significant decrease in cell viability with 50% and 90% inhibitory concentration (IC₅₀ and IC₉₀) of 15.6?±?1.49 and 41.1?±?2.19?μM respectively against the LNCaP cells. This effect was mediated by apoptosis as confirmed by cell cycle arrest at G1-S transition phase, externalization of phosphatidylserine, DNA nicking, loss of mitochondrial membrane potential and reactive oxygen species generation in LNCaP cells. Furthermore, significantly greater cytotoxicity to LNCaP cells was observed with nanoparticles as compared to that of free RL at all tested concentrations. RLPLGA nanoparticles presented no adverse cytotoxic effects on murine macrophages even at 200?μM. Our findings support the potential use of developed resveratrol loaded nanoparticle for the prostate cancer chemoprevention/ chemotherapy with no adverse effect on normal cells.     

Adults with Philadelphia Chromosome–Like Acute Lymphoblastic Leukemia: Considerations for Allogeneic Hematopoietic Cell Transplantation in First Complete Remission

Philadelphia chromosome–like (Ph-like) acute lymphoblastic leukemia (ALL) is a subset of high-risk B cell ALLs. A large proportion of Ph-like ALL cases carry activating kinase mutations that could potentially allow them to be targeted by tyrosine kinase inhibitors. Ph-like ALL is not an uncommon entity, especially among adults, with a frequency exceeding 20%, including in older patients (>60 years old) with ALL. Ph-like ALL is associated with inferior outcomes across all ages, and studies have consistently shown a higher incidence of persistent postinduction minimal residual disease in patients carrying Ph-like ALL compared with other subgroups of ALL, and this translates into inferior leukemia-related outcomes. The inferior outcome of conventional chemotherapy for Ph-like ALL in adults raises the fundamental question of whether all adults with Ph-like ALL require an allogeneic hematopoietic cell transplantation (HCT) in first complete remission (CR1) regardless of other presenting features and treatment response parameters. Here we present and discuss several scenarios in which adults with Ph-like ALL underwent or were considered for HCT in CR1 for various reasons. Although the decision to proceed with HCT was clear and indisputable in some of these situations, in others we struggled with the decision to transplant in CR1 because of the lack of published data regarding the efficacy of allogeneic HCT as consolidation for Ph-like ALL. We emphasize the urgent need for developing well-designed studies to address this important question.     

Successful minimally invasive management of primary maxillary central incisors after lateral luxation: a case report

Lateral luxation in primary maxillary incisors is a traumatic injury that can lead to darkening of the tooth, pulp necrosis, root resorption, and damage to the permanent teeth. Management of this kind of trauma typically includes pulpectomy or extraction in cases of root resorption. However, in young children, removal of the tooth may be psychological stressing, especially for the parents, while increased mobility can make immediate endodontic treatment difficult to perform. This article outlines a severe palatal luxation on the maxillary right central incisor treated with conservative management in a 2-year-old boy. At the one-year follow-up appointment, the surrounding bone was healthy, the roots displayed physiological resorption, the crowns showed color improvement, and there was no radiographically noticeable damage to the permanent teeth.     

Treatment of noncomminuted zygomatic fractures with percutaneous screw reduction and fixation

The standard treatment modality of zygomatic fractures is open reduction and rigid fixation of the fractured segments. Although most of the zygomatic fractures deserve this attentive surgical manipulation to prevent late residual asymmetry, minimally depressed noncomminuted zygomatic fractures can be reduced and fixed percutaneously. Percutaneous intervention causes minimal scarring and morbidity than open techniques and it is possible to align fragments precisely by using high-quality three-dimensional computed tomography (3-D CT) imaging. Six patients with noncomminuted fractures of the zygomaticomaxillary skeleton were evaluated with plain radiographs of facial bones, axial, coronal and 3-D CT. Reduction of the displaced bone segments were achieved by traction of percutaneously applied screw. Either reduced segments were not fixated at all or one of the two new fixation techniques, described in detail in the article, were used for stabilization of reduced segments. In all patients, accurate reduction was obtained. None of the patients showed any recurrent displacement or infection during the follow-up period of six months. The screws were removed in the clinical settings without difficulty. Although percutaneous reduction and external fixation of noncomminuted zygomatic fractures has limited indications, it has its own advantages over open techniques. This method is a less invasive technique and can be performed without any problem in selected cases. Our technique is not suitable for complex zygomatic and periorbital fractures.     

Repeated tissue expansions on split-thickness skin graft in a patient with neurocutaneous syndrome

Soft tissue expansion is a mechanical process that increases the surface area of local tissue available for reconstructive procedures. In most cases, adjacent tissue that matches the recipient site in color, texture, and hair-bearing quality is preferred for tissue expansion. In this particular case with neurocutaneous syndrome, the defects that resulted from removal of parts of a giant hairy nevus overlying the latissimus dorsi muscle bilaterally were grafted with a split-thickness skin graft. Two expanders were then placed under the latissimus dorsi muscles. After full expansion of the grafted area, some part of the remaining nevus surrounding the grafted area was removed and the defects were covered with the expanded skin graft obtained after deflation of the expanders. The expanders placed under the latissimus dorsi muscle in the first operation were reused in the second operation to obtain a second expansion of the skin graft. After the second expansion of the skin graft, the expanders were deflated and another portion of the remaining nevus surrounding the grafted area was removed. The resulting defects were again covered with the excess expanded skin graft. Although repeated expansion of the skin graft is a time-consuming and laborious process, it eliminates the need for taking repeated skin grafts; it decreases skin graft donor site morbidity; it decreases possible infectious complications of tissue expansion by decreasing the number of surgical interventions to the expander pocket; and it increases the aesthetic outcome by keeping all the surgical scars around the grafted area without extending them into healthy surrounding skin.     

The influence of commercially pure titanium and titanium-aluminum-vanadium alloy on the final shade of low-fusing porcelain

AIMS: The aims of this study were to investigate the influence of commercially pure titanium (PTi) and titanium-aluminum-vanadium (Ti-6Al-4V) alloys (TiA) on the final shade of low-fusing porcelain bonded to them and to compare the shade changes with those of three conventional metal-ceramic systems. METHODS AND MATERIALS: A titanium casting unit was used to cast PTi and Ti-6Al-4V alloy specimens followed by A3 shade low-fusing porcelain (Noritake) being bonded to them. Gold-based (AuA), palladium-based (PdA), and nickel-chromium (Ni-Cr) alloys were cast with an automatic centrifugal casting machine, then A3 shade conventional porcelain material (Vita, VMK 95) was applied to them. Ten specimens of each metal were then fabricated. The CIE L* a* b* color coordinates of the specimens were measured with a spectrophotometer. RESULTS: All alloys had significant color changes when compared with A3 shade tabs. The color differences from the shade tabs were 5.79 for the Ti-6Al-4V group, 6.46 for PdA alloy, 8.12 for AuA alloy, 8.15 for Ni-Cr alloy, and 12.58 for PTi. The specimens differed from the shade tabs primarily because of the differences in a* and b* coordinate values. CONCLUSIONS: Predictable shade reproduction of metal-ceramic restorations (MCRs) may be impaired by the underlying metal. The PTi had the greatest color differences among all the tested metal when compared with the shade tabs, whereas the Ti-6Al-4V alloy had the lowest. PTi is more likely to affect the final shade of low-fusing porcelain than Ti-6Al-4V alloy.     

The hyoid bone in malay infants with cleft lip and palate.

OBJECTIVE: To compare morphological and positional variations of the hyoid bone in unoperated infants with cleft lip and palate (CL/P) with those in noncleft infants. DESIGN: Retrospective, cross sectional. PATIENTS AND METHODS: Three-dimensional computed tomography scans were obtained from 29 unoperated CL/P infants of Malay origin aged between 0 and 12 months and from 12 noncleft infants in the same age range. Observations were made and measurements were obtained with a software package developed at the Australian Craniofacial Unit. The sizes of the hyoid bones and the position of the hyoid body and epiglottis in relation to the cervical spine were measured. Anatomical anomalies of the hyoid and prevalence of aspiration pneumonia were also documented. RESULTS: The hyoid bones and epiglottis were found to be located more inferiorly in CL/P infants compared with the noncleft infants. Also, 17% (5/29) of the CL/P infants had nonossified hyoid bodies. CONCLUSION: The results suggest that there are differences in the location and genesis of the hyoid bone in infants with CL/P that warrant further investigation.     

Resistance of core materials against torsional forces on differently conditioned titanium posts

STATEMENT OF THE PROBLEM: The separation of core materials from titanium posts, which have a low modulus of elasticity, has been identified as a problem in restorative dentistry. PURPOSE: This study evaluated the resistance to torsional forces of various core materials adapted to differently conditioned titanium posts. MATERIAL AND METHODS: Seven hundred and twenty specimens were tested consisting of: 6 core materials and 5 surface conditioning techniques for 2 kinds of opaquers with 10 specimens in each group (6 x 5 x 2 x 10=600) plus 6 core materials and 2 opaquers were tested and considered as control groups on nonconditioned titanium posts, each group containing 10 specimens (6 x 2 x 10=120). The custom-made pure titanium posts were conditioned with the following products: Silicoater Classical, Silicoater MD, Rocatec, Kevloc, and Siloc surface-conditioning systems. Subsequently, 6 core materials with different compositions (Durafill, Adaptic, Coradent, Ti-Core, Hytac Aplitip, and Photac-Fil Aplitip) were applied to titanium posts that were previously coated with 2 types of light-polymerized opaquers, either Artglass or Dentacolor. Sixty air-abraded titanium posts (250 microm, 30 seconds) were used as controls for each core material. Following thermocycling (5 degrees -55 degrees C, 30 seconds, 5,000 cycles), maximum torsional forces were determined with an electronic torque movement key. Data were statistically analyzed by 1-way analysis of variance followed by 2-way analysis of variance (P<.05). RESULTS: Significantly higher mean torsional forces were observed with respect to Siloc (20.4 Newton decimeter [dNm]), Silicoater Classical (18.6 dNm), Silicoater MD (18.2 dNm), and Rocatec (17.0 dNm) systems compared with the mean for the untreated control group (14.6 dNm) (P<.001). The Kevloc system (10.4 dNm) demonstrated no significant difference compared with the control group (P>.001). The Kevloc system in combination with the Artglass opaquer and Photac-Fil Aplitip (0.00 dNm) core material showed no resistance against torsional forces. Significant differences were observed between hybrid core materials and microfilled composite, compomer, or resin-modified glass ionomer core materials (P<.001). CONCLUSION:Within the limitations of this study, the resistance to torsional forces for the core materials on titanium posts increased with the use of chemical surface-conditioning techniques and varied in accordance with the opaquer type. Type of core material also significantly influenced the resistance after thermocycling.