肝癌细胞分泌物对凝血系统的直接影响

目的：探讨肝癌细胞分泌物对凝血系统的直接影响。方法：以一期法测定肝癌细胞分泌物对APTT、PT、TT及Ⅷ、Ⅴ因子活性的影响。结果：肝癌细胞分泌物使正常血浆APTT缩短、TT延长、Ⅷ因子活性增加、V因子活性降低。异常血浆PT、TT延长。结论：肝癌细胞分泌物可直接改变血浆凝血像。     

衣霉素对RPMI-8226细胞分化的影响

目的:探讨衣霉素对RPMI-8226细胞分化的机制.方法:用衣霉素处理RPMI-8226细胞.APAAP法测定细胞表面CD49e的表达率;ELISA法测定培养上清液中免疫球蛋白轻链的变化,Western blot法检测XBP-1u蛋白表达水平.结果:衣霉素处理后RPMI-8226细胞表面CD49e表达率增高,细胞培养上...     

血浆灌流治疗腹部开放伤海水浸泡犬肝功能损伤的研究

目的 探讨血浆灌流对腹部开放伤后海水浸泡实验犬肝功能损伤的治疗作用.方法24只腹部开放伤实验犬分为对照组(海水浸泡1.5 h后打捞出水,单纯观察),普通治疗组(海水浸泡后打捞出水,给予一般的急救及补液处理)和血浆灌流组(经海水浸泡后打捞出水,给予一般的急救补液处理,30 h后给予血浆灌注治疗),每组8只.于致伤前及致伤后定时取血测定总胆红素(TB)、血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、凝血酶原时间(PT)、内毒素、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6),同时对肝组织进行病理检查.结果 对照组和普通治疗组血清TB、ALT、AST和LDH明显升高,血浆灌流组灌流后ALT、AST、LDH、TB、内毒素、TNF-α比灌流前明显下降(P<0.05),血细胞、血小板数量与普通治疗组比较明显下降.结论 血浆灌流对外伤后海水浸泡导致的实验犬肝功能损伤的治疗效果良好,此法可应用于该类肝损害的治疗.     

血红素氧合酶-1对移植的异种心脏的保护作用

目的探讨血红素氧合酶-1(HO-1)对移植的异种心脏的保护作用及其可能机制。方法应用NI H小鼠到Wistar大鼠颈部异位心脏移植模型,术前对供者分别用HO-1的诱导剂CoPP或HO-1的阻滞剂ZnPP进行处理,术后受者相应接受CoPP或ZnPP处理,部分受者同时接受环孢素A(CsA)处理,并设空白对照组和单用CsA组。分别用免疫组化、逆转录聚合酶链反应、Westernblot蛋白印迹杂交等检测移植心组织中HO-1、HO-1mRNA、凋亡蛋白酶-3以及信号传导与转录因子-3(STAT-3)的表达,测定心肌组织中HO-1的活性,采用原位末端标记法检测心肌细胞凋亡情况,比较不同处理因素之间的差异。结果单一接受CoPP者(CoPP组)移植心脏存活时间长于空白对照组及单一接受ZnPP者(P<0.01),但短于同时接受CoPP和CsA处理者(CoPP CsA组,P<0.01);CoPP组及CoPP CsA组HO-1、HO-1mRNA的表达及HO-1的活性均高于其余各组(P<0.01),其心肌细胞凋亡数和凋亡蛋白酶-3低于其余各组(P<0.05),而STAT-3高于其余各组(P<0.01)。结论CoPP诱导产生的HO-1可延长NI H小鼠到Wistar大鼠异种移植心脏的存活时间,该作用可能与STAT-3激活、凋亡蛋白酶-3受抑制而导致心肌细胞凋亡发生率下降有关。     

LAMS抑制肝癌细胞增殖和促凝活性的实验研究

目的：观察LAMS抑制肝癌细胞增殖和促凝活性的效果。方法：比较LAMS处理的肝癌细胞生长曲线、AgNoRs含量变化;用一步法APTT测定LAMS拮抗肝癌细胞分泌物的促凝血作用。结果：LAMS处理后的肝癌细胞增殖受抑制、AgNoRs数量减少,LAMS能拮抗肝癌细胞分泌物对APTT的影响。结论：LAMS可有效地抑制肝癌细胞增殖和促凝活性。     

还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

经内镜胆管支架或鼻胆管置放治疗胆管恶性梗阻的临床观察

目的 探讨经十二指肠镜胆管支架或鼻胆管置放治疗胆管恶性梗阻性疾病.方法 有29例胆管恶性梗阻患者接受治疗,成功26例;其中20例被放置塑料支架,6例放置鼻胆管.结果 插管成功率89.7%,并发症3例,其中2例急性胰腺炎、1例急性胆管炎,并发症发生率10.3%.术后1周血清胆红素由290 μmol/L下降到101 μmol/L.3、6个月存活率分别达96%及80%.结论 经内镜胆管支架置放或鼻胆管引流治疗胆管恶性梗阻疾病是安全、有效的方法.     

还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

胶囊内镜对慢性腹痛的诊断分析

目的分析胶囊内镜在慢性腹痛中的诊断价值。方法 2008年9月-2009年7月慢性腹痛25例、无消化道症状体检20例进行胶囊内镜检查,对诊断结果进行相关统计学分析。结果胶囊内镜均顺利完成检查,全部胶囊在检查结束后排出体外。检查过程中患者无任何不适。慢性腹痛患者中,小肠溃疡3例（12%）,小肠息肉3例（12%）,十二指肠扭转1例（4%）,空肠静脉瘤1例（4%）,空肠蛔虫病1例（4%）,回肠黏膜糜烂1例（4%）。无消化道症状的体检者中,小肠息肉2例（10%）,回肠黏膜糜烂1例（5%）。小肠疾病在慢性腹痛患者中检出率40%,明显高于无消化道症状的体检者（15%）,两者比较有统计学意义（P〈0.01）。两组的胃排空时间分别为（40.99±31.38）min和（37.90±24.89）min,到达回盲部时间分别为（220.89±91.64）min和（225.68±80.59）min,两组比较无统计学意义（P〉0.05）。结论胶囊内镜在慢性腹痛的诊断中有良好价值,临床安全性好。