全文获取类型
收费全文 | 368篇 |
免费 | 23篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 90篇 |
儿科学 | 19篇 |
妇产科学 | 14篇 |
基础医学 | 41篇 |
口腔科学 | 11篇 |
临床医学 | 30篇 |
内科学 | 68篇 |
皮肤病学 | 1篇 |
神经病学 | 36篇 |
特种医学 | 11篇 |
外科学 | 33篇 |
综合类 | 5篇 |
预防医学 | 10篇 |
眼科学 | 12篇 |
药学 | 10篇 |
中国医学 | 1篇 |
肿瘤学 | 2篇 |
出版年
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 11篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 9篇 |
2014年 | 8篇 |
2013年 | 15篇 |
2012年 | 33篇 |
2011年 | 25篇 |
2010年 | 11篇 |
2009年 | 6篇 |
2008年 | 22篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 16篇 |
2004年 | 21篇 |
2003年 | 7篇 |
2002年 | 14篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1994年 | 2篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 4篇 |
1969年 | 3篇 |
1968年 | 2篇 |
排序方式: 共有394条查询结果,搜索用时 15 毫秒
1.
E. Angelini M. Teixeira J.-M. Aran E. Ferrary 《European archives of oto-rhino-laryngology》1998,255(7):331-333
Taurine is a β-aminosulfonic acid and is a ubiquitous amino acid whose role in the cochlea is not well established. In this
study, its entry from blood into perilymph was investigated in the guinea pig as animal model. The penetration rate of [3H]taurine (molecular weight 125) into the perilymph of the scala vestibuli was measured 1 and 2 h after the intravenous infusion
of [3H]taurine in nephrectomized animals. Results showed a rate of penetration in perilymph related to plasma at 36 ± 4.7% (n = 5) after 1 h and 43 ± 5.6% (n = 5) after 2 h. Compared to the penetration rate of urea (molecular weight 60) and mannitol (molecular weight 186) reported
previously in rats, a passive entry of taurine into perilymph through the blood-perilymph barrier is suggested.
Received: 30 July 1997 / Accepted: 15 January 1998 相似文献
2.
Contribution of BK Ca2+-activated K+ channels to auditory neurotransmission in the Guinea pig cochlea 总被引:3,自引:0,他引:3
Skinner LJ Enée V Beurg M Jung HH Ryan AF Hafidi A Aran JM Dulon D 《Journal of neurophysiology》2003,90(1):320-332
Large-conductance calcium-activated potassium (BK) channels are known to play a prominent role in the hair cell function of lower vertebrates where these channels determine electrical tuning and regulation of neurotransmitter release. Very little is known, by contrast, about the role of BK channels in the mammalian cochlea. In the current study, we perfused specific toxins in the guinea pig cochlea to characterize the role of BK channels in cochlear neurotransmission. Intracochlear perfusion of charybdotoxin (ChTX) or iberiotoxin (IbTX) reversibly reduced the compound action potential (CAP) of the auditory nerve within minutes. The cochlear microphonics (CM at f1 = 8 kHz and f2 = 9.68 kHz) and their distortion product (DPCM at 2f1-f2) were essentially not affected, suggesting that the BK specific toxins do not alter the active cochlear amplification at the outer hair cells (OHCs). We also tested the effects of these toxins on the whole cell voltage-dependent membrane current of isolated guinea pig inner hair cells (IHCs). ChTX and IbTX reversibly reduced a fast outward current (activating above -40 mV, peaking at 0 mV with a mean activation time constant tau ranging between 0.5 and 1 ms). A similar block of a fast outward current was also observed with the extracellular application of barium ions, which we believe permeate through Ca2+ channels and block BK channels. In situ hybridization of Slo antisense riboprobes and immunocytochemistry demonstrated a strong expression of BK channels in IHCs and spiral ganglion and to a lesser extent in OHCs. Overall, our results clearly revealed the importance of BK channels in mammalian cochlear neurotransmission and demonstrated that at the presynaptic level, fast BK channels are a significant component of the repolarizing current of IHCs. 相似文献
3.
Structures containing gamma-amino butyric acid (GABA) were investigated in the guinea pig cochlear nuclei and superior olivary complexes by means of an immunohistochemical procedure using an antibody directed against GABA. Immunoreactivity was observed in cell bodies of the superficial layers of the ventral and dorsal cochlear nuclei, in lateral superior olive, in some neurons of the medial superior olive, in lateral preolivary nuclei and in the lateral nucleus of the trapezoid body. Fibers and profiles exhibiting GABA immunoreactivity were found in almost all regions of the lower auditory pathways. The abundance of GABA in these regions indicates an important role of this inhibitory amino-acid in the auditory brainstem. 相似文献
4.
Jennifer Mills Nicole Burton Norine Schmidt Oscar Salinas John Hembling Alberto Aran Michele Shedlin Patricia Kissinger 《Journal of immigrant and minority health / Center for Minority Public Health》2013,15(3):606-613
High rates of sex and drug risk behaviors have been documented among Latino migrant men in the US. Whether these behaviors were established in the migrants’ home countries or were adopted in the US has not been described and has implications for prevention strategies. Quarterly surveys were conducted to gather information on selected sex and drug risk practices of Latino migrant men who arrived in New Orleans after Hurricane Katrina seeking work. Both kappa scores and McNemar’s tests were performed to determine if practice of these behaviors in home country was similar to practice post-emigration to the US. Female sex worker (FSW) patronage, same sex encounters (MSM), and crack cocaine use was more likely to occur post- rather than pre-emigration. Of those who ever engaged in these selected behaviors, most adopted the behavior in the US (i.e., 75.8 % of FSW patrons, 72.7 % of MSM participants, and 85.7 % of crack cocaine users), with the exception of binge drinking (26.8 %). Men who were living with a family member were less likely to adopt FSW patronage OR = 0.27, CI = 0.10–0.76, whereas men who earned >$465 per week were more likely to adopt crack cocaine use OR = 6.29 CI = 1.29, 30.57. Interventions that facilitate the maintenance of family cohesion and provide strategies for financial management may be useful for reducing sex and drug risk among newly arrived migrants. 相似文献
5.
T Rispens J Meesters TH den Bleker P Ooijevaar-De Heer J Schuurman PW Parren A Labrijn RC Aalberse 《Molecular immunology》2013,53(1-2):35-42
Human IgG4 antibodies are remarkable not only because they can dynamically exchange half-molecules (Fab-arm exchange) but also for their ability to interact with the Fc part of IgG4 and other IgG subclasses. This rheumatoid factor-like binding of IgG4 does not appear to take place spontaneously, because it is only observed to solid-phase or antigen-bound IgG. We hypothesized that Fc-Fc interactions might involve (partial) dissociation of heavy chains. We investigated the molecular basis of these Fc-Fc interactions, and found that the structural features important for the exchange reaction also control the Fc binding activity. In particular, if arginine-409 in the CH(3)-CH(3) interface in IgG4 is mutated to lysine (the equivalent in IgG1), Fc-Fc interactions are formed 3 orders of magnitude less efficiently compared to the wild-type. This mutation was previously found to increase the CH(3)-CH(3) interaction strength in IgG4. Furthermore, of the two hinge isomers of IgG4, the intra-chain (non-covalently linked) form was found to form Fc-Fc interactions, but not the inter-chain form. Together, these results demonstrate that Fc-Fc interactions of IgG4 involve (partial or complete) dissociation of heavy chains. The promiscuity to other IgG subclasses suggests that IgG4 might act as scavenger to IgG molecules with impaired structural integrity. 相似文献
6.
7.
8.
Svetlana Kostić Ivan Tasić Dragan Mijalković Dragan Lović Dragan Djordjević Todorka Savić Nataša Miladinović Tasić Aleksandra Aranđelović 《Central European Journal of Medicine》2014,9(5):601-608
Abstract
Our aim was to establish the association of carotid intima-media thickness (CIMT) and ankle-brachial index(ABI) with the severity of coronary artery dissease (CAD). The study enrolled 150 examinees and divided them into two groups. The patients with stenotic changes in the coronary artery, constituted the first group (CP)(n=100); the second group consisted of the examinees without CAD — control goup (CG) (n=50). The following methods were used in the study: Color Doppler sonography of the carotid arteries, ABI, calculation of SCORE risk and coronary angiography.Results
The number of coronary blood vessels affected by atherosclerosis was significantly higher with the increase of CIMT, CV risk score, and waist-hip ratio by one measurement unit: CIMT by 0.729; p<0.05; CV risk score by 0.033; p<0.05; and waist-hip ratio by 3.182; p<0.01. With each increase of ABI value by one measurement unit, the number of involved blood vessels dropped by 0.844; p<0.05.Conclusions
Our results demonstrated that reduced ABI value, increased CIMT and number of plaques in the carotid arteries were in correlation with the severity of coronary artery disease. 相似文献9.
10.
Aksoylar Serap Ozgenc Funda Kadioglu Bengu Kantar Mehmet Cetingul Nazan Yagci Rasit Kansoy Savas 《Pediatric transplantation》2011,15(5):E92-E95
Serap A, Funda O, Bengu K, Mehmet K, Nazan C, Rasit Y, Savas K. Sustained seroconversion of chronic hepatitis B infection after stem cell transplantation.Pediatr Transplantation 2011: 15: E92–E95. © 2010 John Wiley & Sons A/S. Abstract: We present an 18‐yr‐old adolescent with acute lymphocytic leukemia, who underwent peripheral blood SCT with serologically and histologically documented chronic hepatitis B infection. Prior and during the transplant process, lamivudine was administered orally and he underwent SCT with a twofold decrease in viral load at the time of transplant from his HLA full matched, HBV natural immune (anti‐HBs and anti‐HBc positive) donor. Successful engraftment was achieved and three months after SCT, HBV seroconversion was documented accompanied with an ALT flare. Chronic graft‐versus‐host disease coincided after the transplantation, and he has been on immunosuppressive treatment for 25 months with sustained HBV seroconversion. We assume that adoptive immunity transfer combined with antiviral treatment might also constitute sustained seroconversion in chronic HBV, besides the reported risk of reactivation. 相似文献