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R. Gil-Grande J. M. Aguado C. Pastor M. García-Bravo C. Gómez-Pellico F. Soriano A. R. Noriega 《European journal of clinical microbiology & infectious diseases》1995,14(1):64-67
A patient with culture-negative endocarditis was diagnosed with Q fever endocarditis based on the results of serological tests and positive leukocyte cultures obtained using conventional viral cultures and the shell vial technique. This case report suggests that isolation ofCoxiella burnetii from blood may allow better diagnostic and therapeutical evaluation of patients with Q fever endocarditis. The use of both conventional and shell vial viral cultures is recommended for the isolation ofCoxiella burnetii from the blood of patients with apparently culture-negative endocarditis. 相似文献
4.
L Zaborski M Taniewski A Krechniak J Pastor 《Bulletin of the Institute of Maritime and Tropical Medicine in Gdynia》1991,42(1-4):79-86
732 workers from several loudest production departments in a pottery plant were examined. The noise level measurements were carried out at a regular course of work. Auditory sensitivity threshold was determined via air conduction, at frequencies of 250, 500, 1000, 2000, 4000 and 8000 Hz. Functioning of the organ of hearing under conditions of noise at the workplace was analysed. The influence of several noise levels--up to 85 dB/A/ and higher, and the relation between the length of service and hearing sensitivity threshold were investigated. The largest exposures were recorded in the batching and milling plant, sorting and kilns departments. 相似文献
5.
Regional lens adenine nucleotide levels were determined in human and bovine lenses using sensitive HPLC techniques. In adult lenses of both species adenine nucleotide levels were highest in the anterior cortex (plus epithelium and capsule) followed by the posterior cortex (plus capsule) and lowest in the nucleus. With increasing lens age ATP level remained steady in the anterior cortex (plus epithelium and capsule) and nucleus, while those in the posterior cortex showed a tendency toward decrease. In both species, human as well as bovine, adenine nucleotide levels of the anterior cortex (plus epithelium and capsule) underwent early postmortem changes. Thus, immediate postmortem sampling as well as regional determinations are a must for detailed studies of lens nucleotide metabolism. 相似文献
6.
Multiclefted nuclei. A helpful feature for identification of intermediate trophoblastic cells in uterine curetting specimens. 总被引:1,自引:0,他引:1
Intermediate trophoblast is a distinct form of trophoblast, the presence of which in uterine curettings is considered a reliable indicator of intrauterine pregnancy even in the absence of chorionic villi. However, the appearance of intermediate trophoblastic cells have not been described in sufficiently specific terms to permit their reliable identification, and distinction from decidual cells can be difficult. We have noticed for some time that the intermediate trophoblastic cells often show multiple deep clefts in the nuclei, and the present study was performed to address the issue of whether this nuclear feature is reliable for their identification. We reviewed 242 uterine curettings of intrauterine pregnancy, documented by the presence of chorionic villi, and were able to find a distinct population of cells with large, hyperchromatic, multiclefted nuclei scattered in the decidua in 88% of the cases. In most instances, these cells produced a characteristic variegated pattern that was readily recognizable at low magnification. Positive immunostaining for cytokeratin (CAM 5.2) in these isolated cells within the decidua confirmed their trophoblastic nature. In contrast, multiclefted nuclei were absent in the 51 negative control cases, which included decidualized endocervical polyps (40 cases), uterine curettings from patients with tubal pregnancy (10 cases), and endometriosis with decidual change (one case). We conclude that intermediate trophoblastic cells can usually be reliably identified in curettings of intrauterine pregnancy by their characteristic nuclear multiclefting. 相似文献
7.
A Cabrera M Sánchez M A Izquierdo E Pastor J M Galdeano S Mintegui M A Lizarraga A Cuadrado M Ca?ada 《Revista espa?ola de cardiología》1992,45(10):637-644
Between 1985-1990 we studied by means of two-dimensional echocardiography 286 patients with isolated ventricular septal defect (VSD). A hundred and sixty three being male and 123 female, as a mean age of 5 +/- 3 months. Sixty two cases (22.3%) reveales associated anomalies and in other 63 (22.4%) there was in the evolution an aneurysm of the septum membranous. The projections used were apical 4-chamber, with or without aortic root; parasternal long-short axis of great arterias or at ventricular level; subcostal 4-chamber; right oblique outlet right outflow tract and left oblique outlet left and right outflow tracts. Patients with VSD smaller than 3 mm were excluded. Perimembranous defects, 189 cases (66%), were more frequent than muscular, 91 31.8%), and subarterial defects, 6 (2.1%). Only 19 (9 apical muscular, 6 perimembranous outlet and 4 perimembranous trabecular) were initially misclassified. The diameter of the VSD was large in subarterial (0.85 +/- 0.1 cm) and perimembranous outlet VSD (0.75 +/- 0.5 cm) compared with the rest. Each group of defects was more easily shown by one particular projection except trabecular muscular defect. In conclusion, we are able to state that two-dimensional echocardiography enables us to discover the size and situation of isolated ventricular defects. 相似文献
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Spleen cells of a Biozzi HR mouse immunized with a bovine serum albumin-methotrexate conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells. Twenty-three monoclonal antibodies (MAbs), selected by indirect ELISA, were produced and partially characterized. Using methotrexate (MTX) and eight structurally related compounds, binding specificities of the MAbs were assessed by inhibition enzyme immunoassay. All the MAbs had very low affinity for folic acid and its analogs and for the major MTX metabolite 7-hydroxymethotrexate. Using a computer cluster analysis program based on the binding specificities, the MAbs were divided into three groups. The thirteen MAbs in group I recognized primarily the pteridine portion of the MTX molecule; the eight group II MAbs recognized the benzene ring as well as the pteridine structure. The two MAbs in group III poorly distinguished between the different parts of the MTX molecule. The apparent equilibrium association constants of the anti-MTX MAbs in groups I, II, and III ranged from 7 x 10(9) to 3 x 10(8) M-1 (except for 1 MAb), from 5 x 10(7) to 6 x 10(6) M-1 (except for 2 MAbs), and from 1 x 10(6) to 3.5 x 10(5) M-1, respectively. 相似文献
10.
H Pau 《Klinische Monatsbl?tter für Augenheilkunde》1988,193(6):550-553
The three important types of age-related senile cataract, namely deep supranuclear cortical cataract, superficial subcapsular cortical cataract (sometimes associated with secondary nuclear cataract), and primary nuclear cataract, are arranged in a table which may be used for quick reference with regard to clinical features - slit lamp findings, age at onset of opacification, rate of progression, and related biochemistry (e.g., ATP content, glutathione content, and the cation pump (K+ greater than Na+] - as well as etiological features. The significance of the table is illustrated on the basis of an important example from the literature ("Sunlight and Human Cataracts"). 相似文献