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排序方式: 共有416条查询结果,搜索用时 15 毫秒
1.
Hemodynamics in the choriocapillaris in pulseless disease 总被引:1,自引:0,他引:1
2.
Toshiro Takatori Takeshi Koizumi Taro Tokui Yoshihiro Mitsuhashi Akio Shiraishi Takashi Tsuruo 《Cancer chemotherapy and pharmacology》1995,35(4):283-290
RS-1541, an acyl-derivative of rhizoxin (Fig. 1), is a potent antitumor compound. This agent showed cytotoxicity in vitro on some cultured human tumor cells, although it was less potent than rhizoxin. Rhizoxin exhibited antitumor effects by inhibiting the polymerization of tubulin, whereas RS-1541 did not inhibit tubulin polymerization in vitro. However, cell cycle analysis in vivo showed that the two agents had the same mode of action. The cytotoxicity of RS-1541 was enhanced when the initial cell density of the cells was increased. The cytotoxicity was also enhanced when the membrane fraction of St-4 cells, which were the most sensitive to RS-1541 among the cell lines tested, was added to the target cells. When St-4 cells were incubated with [14C]-RS-1541, significant amounts of [14C]-rhizoxin were produced within the cells. Further fractionation of the crude membrane showed that the activity that enhanced the cytotoxicity of RS-1541 (RS-1541-enhancing activity) belonged to the mitochondrial-lysosomal fraction, not to the microsomal fraction. Both the enhancing activity and the activity that converting [14C]-RS-1541 to [14C]-rhizoxin (RS-1541-converting activity) were inhibited by treatment with chloroquine, an inhibitor of lysosomal function. Cholesterol esterase derived fromCandida cylindracea had RS-1541-enhancingand-converting activities. These data suggest that RS-1541 exerts its cytotoxic action after being converted to rhizoxin within the cells by a lysosomal enzyme such as cholesterol esterase.Abbreviations
DMSO
Dimethylsulfoxide
-
PBS(-) Ca2+
Mg2+-free phosphate-buffered saline
-
HCO60
hydrogenated castor oil polyethylene glycor ether
-
DMA
dimethylacetamide
-
RSB
reticulocyte standard buffer, consisting of 10mM NaCl, 1.5 mM MgCl2, and 10 mM TRIS-HCl, (pH 7.4)
-
TLC
thin-layer chromatography
-
ara-C
1--D-arabinofuranosylcytosine
-
LDL
low-density lipoprotein 相似文献
3.
Nafamostat mesilate suppresses NF-kappaB activation and NO overproduction in LPS-treated macrophages 总被引:2,自引:0,他引:2
Noguchi S Nakatsuka M Konishi H Kamada Y Chekir C Kudo T 《International immunopharmacology》2003,3(9):1335-1344
Nafamostat mesilate (NM), a clinically used serine protease inhibitor, suppressed the overproduction of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) in RAW264.7 murine macrophages treated with lipopolysaccharide (LPS, 100 ng/ml); however, it had little effect on endothelial NOS (eNOS) in human umbilical vein endothelial cells (HUVEC). Electrophoretic mobility shift assay (EMSA) revealed that LPS activated nuclear factor-kappaB (NF-kappaB) in RAW264.7 cells and that this activation was suppressed by nafamostat mesilate. Western blotting showed that nafamostat mesilate suppressed the phosphorylation and degradation of inhibitor kappaB-alpha (IkappaB-alpha), which holds NF-kappaB in the cytoplasm in an inactivated state. Our observations suggest that nafamostat mesilate is a candidate agent for various diseases such as ischemia-reperfusion, graft rejection, inflammatory diseases, and autoimmune diseases, in which iNOS and/or NF-kappaB are upregulated. 相似文献
4.
Taku Oishi Mikiya Fujieda Taisuke Shiraishi Miki Ono Kazuo Inoue Akiyoshi Takahashi Hideo Ogura Hiroshi Wakiguchi 《Circulation journal》2008,72(5):850-852
Tumor necrosis factor-alpha (TNF-alpha) is considered to be 1 of the factors that induce vasculitis, including coronary artery aneurysm (CA), in Kawasaki disease (KD), because the blood concentration of TNF-alpha is higher in patients with CA compared with those without. Therefore, an anti-TNF-alphaagent (infliximab) was administered to a 1-month-old girl with refractory KD complicated by CA and subsequently, the CA improved and KD was controlled without complications 20 months after the onset. 相似文献
5.
PVA hydrogels with anisotropic structures have many biomedical applications; however, the hydrophilicity of PVA nanofibers degrades their mechanical properties, and the residual unreacted chemical crosslinkers are disadvantageous for medical use. Therefore, maintaining the stability of aqueous solutions without using crosslinkers is essential while synthesizing electrospun anisotropic PVA nanofibers. Herein, we developed a novel fabrication method for synthesizing tough, anisotropic, and chemical-crosslinker-free nanofibrous cryogels composed of poly(vinyl alcohol) (PVA) and glycerol (Gly) via electrospinning in conjunction with freeze–thawing treatment. Wide-angle X-ray diffraction, attenuated total reflection Fourier-transform infrared spectroscopy, and differential scanning calorimetry analysis revealed an enhanced crystallinity of the PVA and hydrogen bonds in the PVA/Gly nanofibers after freeze–thawing, thereby leading to improved stability of the PVA/Gly nanofiber in water. The scanning electron microscopy observation and tensile tests revealed that the addition of Gly improved both the orientation and the mechanical properties. The values of the toughness parallel and vertical to the fiber axis direction were 4.20 ± 0.63 MPa and 2.17 ± 0.27 MPa, respectively, thus revealing the anisotropy of this mechanical property. The PVA/Gly nanofibrous cryogel consisted of physically crosslinked biocompatible materials featuring toughness and mechanical anisotropy, which are favorable for medical applications including tissue engineering.Fabrication of tough, anisotropic, and chemical crosslinker-free nanofibrous cryogels made from poly(vinyl alcohol) and glycerol via electrospinning in conjunction with freeze-thawing treatment which would be favorable for medical applications. 相似文献
6.
Hisashi Kaneda Masaki Shimizu Kazuhide Ohta Katsumi Ushijima Yoshimitsu Gotoh Kenichi Satomura Takuhito Nagai Mikiya Fujieda Masashi Morooka Takuji Yamada Masayoshi Yamada Naohiro Wada Mari Takaai Yukiya Hashimoto Osamu Uemura 《Clinical and experimental nephrology》2016,20(5):757-763
Background
The present study aimed to obtain information enabling optimisation of the clinical effect of mizoribine (MZR) in pediatric patients with kidney disease.Methods
A total of 105 pediatric patients with kidney disease treated at our institutions were enrolled. Kidney transplant patients were excluded. Population pharmacokinetic analysis of MZR was performed based on serum concentration data. Area under the curve from time zero to infinity (AUC∞) and maximal concentration (C max) were calculated by Bayesian analysis.Results
In children, the appearance of MZR in the blood tended to be slower and the subsequent rise in blood concentration tended to be more sluggish, compared to healthy adults. Apparent volume of distribution and oral clearance were also higher in children compared to adults. A significant positive correlation was observed between patient age and AUC∞. There were significant differences of AUC∞ and C max by age group. No relationship was observed between the administration method of MZR and serum concentration.Conclusion
The pharmacokinetics of MZR was different in children compared to adults. To obtain the expected clinical efficacy, the regular MZR dosage schedule (2–3 mg/kg/day) might be insufficient for pediatric patients. In particular, younger patients might require a higher dosage of MZR per unit body weight.7.
Mikiya Inoue Kiminobu Sugito Taro Ikeda Hiroyuki Kawashima Manabu Hanada Takeshi Furuya Kensuke Ohashi Tsugumichi Koshinaga 《Journal of gastrointestinal surgery》2014,18(3):580-583
Background
The present study aimed to assess the long-term results of seton placement for fistula-in-ano (FIA) in infants.Methods
Data of patients aged <1 year who presented to our department with perianal abscess (PA) between January 2006 and February 2010 were retrospectively reviewed. Our standard initial treatment for PA was incision and drainage. Patients with systemic diseases and inflammatory bowel diseases were excluded.Results
Ninety-five patients were treated for PA and/or FIA during the 5-year period, and follow-up data were available for 90 patients. The mean follow-up duration in these patients was 49.8?±?11.4 months, and mean age at presentation was 3.1?±?2.7 months. Of the 90 patients, 36 (40 %) developed FIA (39 lesions) and underwent seton placement. The condition healed in a mean period of 6.3?±?4.0 weeks after the placement of a cutting seton. Healing of the fistula was achieved in 35 (97.2 %) of 36 patients after the initial seton procedure, and one patient who showed recurrence underwent a second seton placement, resulting in successful healing of the FIA after 5 weeks.Conclusions
The long-term success of seton placement indicates that this procedure should be a treatment option for FIA in infants. 相似文献8.
9.
Shinji Kanemitsu Keizo Tanaka Hitoshi Suzuki Toshiya Tokui Toshihiko Kinoshita 《Circulation journal》2006,70(5):638-640
It is necessary to use side clamps to construct proximal anastomoses in off-pump coronary artery bypass, and this can be related to neurologic complications. Recently a new device, the HEARTSTRING device, was developed. We present a 78-year-old man who underwent emergent bypass surgery using the HEARTSTRING device to avoid a side clamp. We found atherosclerotic debris from the punched hole and, unfortunately, a postoperative neurological complication resulted. We strongly suggest that it is most important that potential candidates for the HEARTSTRING device be carefully selected to reduce possible neurologic complications. We report that while this new device is useful, there is a potential pitfall in using it; that it is a possible source of atheroembolism. 相似文献
10.
Yoichi Morinishi Kohsuke Imai Noriko Nakagawa Hiroki Sato Katsuyuki Horiuchi Yoshitoshi Ohtsuka Yumi Kaneda Takashi Taga Hiroaki Hisakawa Ryosuke Miyaji Mikiya Endo Tsutomu Oh-ishi Yoshiro Kamachi Koshi Akahane Chie Kobayashi Masahiro Tsuchida Tomohiro Morio Yoji Sasahara Satoru Kumaki Keiko Ishigaki Makoto Yoshida Tomonari Urabe Norimoto Kobayashi Yuri Okimoto Janine Reichenbach Yoshiko Hashii Yoichiro Tsuji Kazuhiro Kogawa Seiji Yamaguchi Hirokazu Kanegane Toshio Miyawaki Masafumi Yamada Tadashi Ariga Shigeaki Nonoyama 《The Journal of pediatrics》2009,155(6):829-833