排序方式: 共有39条查询结果,搜索用时 31 毫秒
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Peter Bergin Lynette Sadleir Benjamin Legros Zarine Mogal Manjari Tripathi Nitika Dang Simone Beretta Clara Zanchi Jorge Burneo Thomas Borkowski Yang Je Cho Michel Ossemann Pasquale Striano Kavita Srivastava Hui Jan Tan Jithangi Wanigasinghe Wendyl D’Souza for the EpiNet study group 《Epilepsia》2012,53(10):1829-1835
Purpose: We created an epilepsy patient database that can be accessed via the Internet by neurologists from anywhere in the world. The database was designed to enroll and follow large cohorts of patients with specific epilepsy syndromes, and to facilitate recruitment of patients for investigator‐initiated clinical trials. Methods: The EpiNet database records physician‐derived information regarding seizure type and frequency, epilepsy syndrome, etiology, drug history, and investigations. It can be accessed from any country by approved investigators via a secure, password‐protected Website. All data are encrypted. The database is for both research and clinical purposes. Investigators were invited to register any patient with epilepsy, but were particularly encouraged to register patients when uncertain of the optimal management. Participation required approval from investigators’ ethics committees and institutional review boards, and all patients or their caregiver provided written informed consent. Patients were not enrolled in clinical trials in this pilot study. Key Findings: The international pilot study recruited patients from September 2010 to November 2011. Sixty‐four investigators or research assistants from 25 centers in 13 countries registered 1,050 patients. Patients with a wide range of epilepsy syndromes and etiologies were registered. Patients’ ages ranged from 2 weeks to 90 years. Significance: The Website was successfully used by doctors working in different health systems. The pilot study confirmed that this low‐cost, collaborative approach to research has great potential. Large, multicenter cohort studies will commence in 2012, and randomized clinical trials are being planned. All epileptologists are invited to join this project. 相似文献
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Prognostic significance of morphological growth patterns and mitotic index of epithelioid malignant peritoneal mesothelioma 下载免费PDF全文
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Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes 总被引:1,自引:0,他引:1
In quantitative RT-PCR (qRT-PCR), analysis of gene expression is dependent on normalization using housekeeping genes such as 18S rRNA, GAPDH and beta actin. However, variability in their expression has been reported to be caused by factors like drug treatment, pathological states and cell-cycle phase. An emerging area of cancer research focuses on identifying the role of epigenetic alterations such as histone modifications and DNA methylation in the initiation and progression of cancer. Histone acetylation is the best studied modification so far and has been probed through the use of histone deacetylase inhibitors (HDACi). Further, modulation of histone acetylation is currently being explored as a therapeutic strategy in the treatment of cancer and HDACis have shown promise in inhibiting tumorigenesis and metastasis. Trichostatin-A (TSA) is the most widely used HDACi. Therefore, we were driven to identify a suitable internal control for RT-PCR following TSA treatment. We performed quantitative RT-PCR analysis using mouse prostate tissue explants, human prostate cancer (LNCaP) cells and human breast cancer (T-47D and ZR-75-1) cells following TSA treatment. Expression of housekeeping genes including 18S rRNA, beta actin, GAPDH and ribosomal highly-basic 23-kDa protein (rb 23-kDa, RPL13A) were compared in vehicle versus TSA treated samples. Our results showed marked variations in 18S rRNA, beta actin mRNA and GAPDH mRNA levels in mouse prostate explants and a human prostate cancer (LNCaP) cell line following TSA treatment. Furthermore, in two human breast cancer cell lines (T-47D and ZR-75-1) 18S rRNA, beta actin mRNA and GAPDH mRNA levels varied significantly. However, RPL13A mRNA levels remained constant in all the conditions tested. Therefore, we recommend use of RPL13A as a standard for normalization during TSA treatment. 相似文献
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Jennifer L Leiting Jordan M Cloyd Ahmed Ahmed Keith Fournier rew J Lee Sophie Dessureault Seth Felder Jula Veerapong Joel M Baumgartner Callisia Clarke Harveshp Mogal Charles A Staley Mohammad Y Zaidi Sameer H Patel Syed A Ahmad Ryan J Hendrix Laura Lambert Daniel E Abbott Courtney Pokrzywa Mustafa Raoof Christopher J LaRocca Fabian M Johnston Jonathan Greer Travis E Grotz 《World journal of gastrointestinal oncology》2020,12(7):756-767
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Association of perioperative transfusion with survival and recurrence after resection of gallbladder cancer: A 10‐institution study from the US Extrahepatic Biliary Malignancy Consortium 下载免费PDF全文
Alexandra G. Lopez‐Aguiar MD Cecilia G. Ethun MD Mia R. McInnis BA Timothy M. Pawlik MD MPH PhD George Poultsides MD Thuy Tran MD Kamran Idrees MD Chelsea A. Isom MD Ryan C. Fields MD Bradley A. Krasnick MD Sharon M. Weber MD Ahmed Salem MD Robert C. G. Martin MD Charles R. Scoggins MD Perry Shen MD Harveshp D. Mogal MD Carl Schmidt MD Eliza W. Beal MD Ioannis Hatzaras MD Rivfka Shenoy MD Kenneth Cardona MD Shishir K. Maithel MD 《Journal of surgical oncology》2018,117(8):1638-1647
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John H. Stewart Dan G. Blazer Maria Jose Godoy Calderon Taylor M. Carter Austin Eckhoff Mohammad A. Al Efishat Deemantha G. Fernando Jason M. Foster Andrea Hayes-Jordan Fabian M. Johnston Timothy B. Lautz Edward A. Levine Ugwuji N. Maduekwe Christopher W. Mangieri Omeed Moaven Harveshp Mogal Perry Shen Konstantinos I. Votanopoulos 《Current problems in surgery》2021,58(4):100860
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The prognostic utility of baseline alpha‐fetoprotein for hepatocellular carcinoma patients 下载免费PDF全文
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Assessing the impact of common bile duct resection in the surgical management of gallbladder cancer 下载免费PDF全文
Faiz Gani MBBS Stefan Buettner BS Georgios A. Margonis MD PhD Cecilia G. Ethun MD George Poultsides MD Thuy Tran MD Kamran Idrees MD Chelsea A. Isom MD Ryan C. Fields MD Bradley Krasnick MD Sharon M. Weber MD Ahmed Salem MD Robert C.G. Martin MD Charles Scoggins MD Perry Shen MD Harveshp D. Mogal MD Carl Schmidt MD Eliza Beal MD Ioannis Hatzaras MD Rivfka Shenoy MD Shishir K. Maithel MD Timothy M. Pawlik MD MPH PhD 《Journal of surgical oncology》2016,114(2):176-180