Background
Blood donation in Morocco and more particularly in the northwest region is carried out without prior determination of the pre-donation hemoglobin. In addition, we note the lack of scientific research that reports data on the red blood cells, leukocytes and platelet lines in donated blood at the regional or even national level.Aims
To study hemogram profile in blood donors taken from the Northwest region of Morocco in order to provide decision makers of the National Center of Blood Transfusion and Hematology with valid scientific arguments to complete the criteria to donate whole blood, by the hemogram.Methods
Prospective study, conducted in 15797 volunteer blood donors (BD) aged between 18 and 60 years, collected during mobile or fixed collections carried out by the Regional Blood Transfusion Center of Tangier and Tetouan from November 2014 to May 2016. The hemogram was performed using a Sysmex KX21N® and the analysis of the data was done by the software SPSS 20.0.Results
According to the World Health Organization, anemia corresponds to a hemoglobin level less than 12 g/dL in women and less than 13 g/dL in men. We found that 14.5 % of women (n = 1054) and 3.0 % of men (n = 245) were anemic and anemia was hypochromic microcytic in 58,66 % of these BD. Analysis of the white line showed leucopenia in 2.05 % of BD and 807 cases of leukocytosis (5.27 % of BD). Platelet study showed thrombocytopenia in 3.97 % of BD and thrombocytosis in 151BD (0.99 % of cases).Conclusion
This study shows the interest of systematic pre-donation hemoglobin measurement and periodic realization of the hemogram among BD in the Northwest region of Morocco. 相似文献The formation of Advanced Glycation Endproducts (AGEs) has been found to play a role in the development of diabetic symptoms. Production of methylglyoxal (MG), a highly cytotoxic and crosslinking aldehyde, is elevated among patients with type 2 diabetes mellitus (T2DM) and is a precursor to AGEs. The ubiquitous glyoxalase system is one of several defense mechanisms involved in MG metabolism and the protection against the production of AGEs. The system is a complex of two enzymes: glyoxalase I (GloI) that converts MG and reduced glutathione (GSH) to S-lactoylglutathione which is converted to D-lactic acid by glyoxalase II, regenerating GSH in the process. The malfunctioning of the glyoxalase system results in the accumulation of MG. The present study was performed to explore the relationship between the decreased activity of GloI and the complications associated to T2DM. The activities of the GloI, GloII and the concentration of GSH were measured in blood samples of 203 volunteers: 75 controls, 60 non-insulino-dependent diabetes mellitus (NIDDM) individuals and 68 NIDDM patients with complications as follow: 18 with nephropathy, 15 with retinopathy, 15 with neuropathy and 20 with macroangiopathy. All individuals were from the northen region of Morocco. We also evaluated the relationships between GloI levels and the pathogenesis of micro- and macrovascular complications of diabetes. We found a significant decrease in the GloI activity and GSH levels in patients with diabetes compared to controls. GloI activity was further reduced in samples from diabetes patients with complications. The levels of GloI were markedly lower in blood samples from patients with nephropathy than in uncomplicated patients and normal subjects. In contrast, there was no significant change in the activity of GloII in NIDDM patients compared to controls. This study suggests that the low level of GloI activity in T2DM patients is most probably due to decreased level of GSH content and reflects the role of GloI enzyme in protecting T2DM patients from AGEs accumulation and further complications.
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